##gff-version 3
##sequence-region BBa_K539151 1 4767
BBa_K539151	.	engineered_region	3437	4767	.	+	0	ID=BBa_K539138;Name=BBa_K539138
BBa_K539151	.	mature_transcript_region	3437	3488	.	+	0	ID=BBa_K115002;Name=BBa_K115002;Parent=BBa_K539138
BBa_K539151	.	ribosome_entry_site	3483	3488	.	+	0	ID=annotation1966897;Name=rbs;Parent=BBa_K115002
BBa_K539151	.	stem_loop	3460	3488	.	+	0	ID=annotation1966931;Name=predicted stem-loop extending to one position before start codon;Parent=BBa_K115002
BBa_K539151	.	sequence_alteration	3459	3464	.	+	0	ID=annotation1966899;Name=scar adaptation;Parent=BBa_K115002
BBa_K539151	.	stem_loop	3437	3454	.	+	0	ID=annotation1966930;Name=stem_loop;Parent=BBa_K115002
BBa_K539151	.	CDS	3495	4646	.	+	0	ID=BBa_K539119;Name=BBa_K539119;Parent=BBa_K539138
BBa_K539151	.	CDS	3495	4643	.	+	0	ID=annotation2131423;Name=crtY coding sequence;Parent=BBa_K539119
BBa_K539151	.	engineered_region	3495	4646	.	+	0	ID=annotation2131424;Name=BBa_K118008;Parent=BBa_K539119
BBa_K539151	.	CDS	3495	4646	.	+	0	ID=BBa_K118008;Name=BBa_K118008;Parent=BBa_K539138
BBa_K539151	.	CDS	3495	4643	.	+	0	ID=annotation1995716;Name=crtY coding sequence;Parent=BBa_K118008
BBa_K539151	.	terminator	4655	4767	.	+	0	ID=BBa_J61048;Name=BBa_J61048;Parent=BBa_K539138
BBa_K539151	.	engineered_region	1	3428	.	+	0	ID=BBa_K346090;Name=BBa_K346090
BBa_K539151	.	promoter	1	35	.	+	0	ID=BBa_J23103;Name=BBa_J23103;Parent=BBa_K346090
BBa_K539151	.	engineered_region	44	3428	.	+	0	ID=BBa_K274100;Name=BBa_K274100;Parent=BBa_K346090
BBa_K539151	.	CDS	990	1922	.	+	0	ID=BBa_K118002;Name=BBa_K118002;Parent=BBa_K274100
BBa_K539151	.	CDS	990	1919	.	+	0	ID=annotation1995701;Name=crtB coding sequence;Parent=BBa_K118002
BBa_K539151	.	CDS	54	965	.	+	0	ID=BBa_I742151;Name=BBa_I742151;Parent=BBa_K274100
BBa_K539151	.	CDS	1947	3428	.	+	0	ID=BBa_K118003;Name=BBa_K118003;Parent=BBa_K274100
BBa_K539151	.	point_mutation	2424	2429	.	+	0	ID=annotation1968244;Name=PstI site removed;Parent=BBa_K118003
BBa_K539151	.	CDS	1947	3425	.	+	0	ID=annotation1995702;Name=crtI coding sequence;Parent=BBa_K118003
BBa_K539151	.	point_mutation	2835	2840	.	+	0	ID=annotation1968245;Name=PstI site removed;Parent=BBa_K118003
BBa_K539151	.	ribosome_entry_site	44	47	.	+	0	ID=BBa_K118012;Name=BBa_K118012;Parent=BBa_K274100
BBa_K539151	.	ribosome_entry_site	974	983	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_K274100
BBa_K539151	.	ribosome_entry_site	977	983	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_K539151	.	sequence_feature	974	976	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_K539151	.	ribosome_entry_site	1931	1940	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_K274100
BBa_K539151	.	ribosome_entry_site	1934	1940	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_K539151	.	sequence_feature	1931	1933	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
>BBa_K539151
ctgatagctagctcagtcctagggattatgctagctactagaggaggtactagatgacggtctgcgcaaaaaaacacgt
tcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggaggga
gaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgc
tgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgc
ggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcat
tacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcc
tcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagtt
caaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctg
ttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcac
ttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcagga
cgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgcc
agtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcg
ctgccgtcagttaataatactagagctcaaggaggtactagatgaataatccgtcgttactcaatcatgcggtcgaaac
gatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctc
tacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaa
cgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtt
tgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatg
gatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttga
tgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgac
caatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggt
ctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcag
aaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagca
ggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgccc
gaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgc
atctctggcagcgcccgctctaataatactagagctcaaggaggtactagatgaaaccaactacggtaattggtgcagg
cttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaaccc
ggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtgcca
ttgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccgcct
gtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaatccc
cgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggtactg
tcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactgcaagcatggagaagcgtttacag
taaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaatccc
ttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggcaccg
gcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagccatat
ggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtcaaat
gcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagcagtccaacaaactgcaaacta
agcgcatgagtaactctctgtttgtgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggtttg
tttcggcccgcgttaccgcgagctgattgacgaaatttttaatcatgatggcctcgcagaggacttctcactttatctg
cacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcagttactatgtgttggcgccggtgccgcatt
taggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgaccgtatttttgcgtaccttgagcagcatta
catgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgtttgattttcgcgaccagcttaatgcctat
catggctcagccttttctgtggagcccgttcttacccagagcgcctggtttcggccgcataaccgcgataaaaccatta
ctaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcatcggctcggcaaaagcgacagc
aggtttgatgctggaggatctgatataataatactagagggacaagcaatgcttgccttgaatagtaacttttgaatag
tgattcaggaggtactagatgcaaccgcattatgatctgattctcgtgggggctggactcgcgaatggccttatcgccc
tgcgtcttcagcagcagcaacctgatatgcgtattttgcttatcgacgccgcaccccaggcgggcgggaatcatacgtg
gtcatttcaccacgatgatttgactgagagccaacatcgttggatagctccgctggtggttcatcactggcccgactat
caggtacgctttcccacacgccgtcgtaagctgaacagcggctacttttgtattacttctcagcgtttcgctgaggttt
tacagcgacagtttggcccgcacttgtggatggataccgcggtcgcagaggttaatgcggaatctgttcggttgaaaaa
gggtcaggttatcggtgcccgcgcggtgattgacgggcggggttatgcggcaaattcagcactgagcgtgggcttccag
gcgtttattggccaggaatggcgattgagccacccgcatggtttatcgtctcccattatcatggatgccacggtcgatc
agcaaaatggttatcgcttcgtgtacagcctgccgctctcgccgaccagattgttaattgaagacacgcactatattga
taatgcgacattagatcctgaatgcgcgcggcaaaatatttgcgactatgccgcgcaacagggttggcagcttcagaca
ctgctgcgagaagaacagggcgccttacccattactctgtcgggcaatgccgacgcattctggcagcagcgccccctgg
cctgtagtggattacgtgccggtctgttccatcctaccaccggctattcactgccgctggcggttgccgtggccgaccg
cctgagtgcacttgatgtctttacgtcggcctcaattcaccatgccattacgcattttgcccgcgagcgctggcagcag
cagggctttttccgcatgctgaatcgcatgctgtttttagccggacccgccgattcacgctggcgggttatgcagcgtt
tttatggtttacctgaagatttaattgcccgtttttatgcgggaaaactcacgctgaccgatcggctacgtattctgag
cggcaagccgcctgttccggtattagcagcattgcaagccattatgacgactcatcgttaataatactagagccggctt
atcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgac
tgtccacgacgctatacccaaaagaaa