BBa_K539281
type: ComponentDefinition
persistent identity: BBa K539281
display id: BBa_K539281
version: 1
was generated by: iGEM to SBOL conversion
description: rbs+crtZ(beta-carotene hydroxylase) under heat sensitive cI regulater
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: crtZ coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087)
owned by: james
owned by: myers
mutable description: This part contains crtZ, which works as the zeaxanthin biosynthesis pathway when add beta-carotene as a substrate. crtZ is regulated by cI and cI regulated promoter. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription.
discontinued: false
dominant: false
experience: experience/Works
group u list: _705_
m user id: 0
owner id: 10230
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: This operon is under a heat sensitive cI QPI with a high promoter preceding the repressor (BBa_J23114).
star: false
creator: Yang, Yi-Pei
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J61048
component: BBa_I742158
component: BBa_K098995
sequence annotation: BBa_K098995
sequence annotation: BBa_I742158
sequence annotation: BBa_J61048
sequence: https://synbiohub.org/public/igem/BBa_K539281_sequence/1

BBa_J61048
type: Component
persistent identity: component2227650
display id: component2227650
version: 1
top level: BBa_K539281
definition: BBa_J61048
access: public

BBa_I742158
type: Component
persistent identity: component2227649
display id: component2227649
version: 1
top level: BBa_K539281
definition: rbs+crtZ
access: public

BBa_K098995
type: Component
persistent identity: component2227645
display id: component2227645
version: 1
top level: BBa_K539281
definition: BBa_K098995
access: public

BBa_K098995
type: SequenceAnnotation
persistent identity: annotation2227645
display id: annotation2227645
version: 1
top level: BBa_K539281
location: https://synbiohub.org/public/igem/BBa_K539281/annotation2227645/range2227645/1
component: BBa_K098995

https://synbiohub.org/public/igem/BBa_K539281/annotation2227645/range2227645/1
type: Range
persistent identity: range2227645
display id: range2227645
version: 1
top level: BBa_K539281
direction: direction/reverse
start: 1
end: 929
orientation: inline

BBa_I742158
type: SequenceAnnotation
persistent identity: annotation2227649
display id: annotation2227649
version: 1
top level: BBa_K539281
location: https://synbiohub.org/public/igem/BBa_K539281/annotation2227649/range2227649/1
component: BBa_I742158

https://synbiohub.org/public/igem/BBa_K539281/annotation2227649/range2227649/1
type: Range
persistent identity: range2227649
display id: range2227649
version: 1
top level: BBa_K539281
direction: direction/reverse
start: 938
end: 1484
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2227650
display id: annotation2227650
version: 1
top level: BBa_K539281
location: https://synbiohub.org/public/igem/BBa_K539281/annotation2227650/range2227650/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K539281/annotation2227650/range2227650/1
type: Range
persistent identity: range2227650
display id: range2227650
version: 1
top level: BBa_K539281
direction: direction/reverse
start: 1493
end: 1605
orientation: inline

rbs+crtZ
type: ComponentDefinition
persistent identity: BBa I742158
display id: BBa_I742158
version: 1
was generated by: iGEM to SBOL conversion
description: crtZ (beta-carotene hydroxylase) with native rbs
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: crtZ (beta-carotene hydroxylase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis pathway. Converts beta-carotene to the yellow pigment zeaxnathin.
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: In stock
status: status/Available
bookmark: true
mutable notes: This part was made as a single biobrick rather than separate rbs and coding sequence, hence the use of blunt combination to generate the final sequence.
star: true
creator: Chris French
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
component: BBa_I742157
component: BBa_I742156
sequence annotation: BBa_I742156
sequence annotation: BBa_I742157
sequence: https://synbiohub.org/public/igem/BBa_I742158_sequence/1

BBa_I742157
type: Component
persistent identity: component1953815
display id: component1953815
version: 1
top level: rbs+crtZ
definition: BBa_I742157
access: public

BBa_I742156
type: Component
persistent identity: component1953813
display id: component1953813
version: 1
top level: rbs+crtZ
definition: BBa_I742156
access: public

BBa_I742156
type: SequenceAnnotation
persistent identity: annotation1953813
display id: annotation1953813
version: 1
top level: rbs+crtZ
location: https://synbiohub.org/public/igem/BBa_I742158/annotation1953813/range1953813/1
component: BBa_I742156

https://synbiohub.org/public/igem/BBa_I742158/annotation1953813/range1953813/1
type: Range
persistent identity: range1953813
display id: range1953813
version: 1
top level: rbs+crtZ
direction: direction/reverse
start: 1
end: 16
orientation: inline

BBa_I742157
type: SequenceAnnotation
persistent identity: annotation1953815
display id: annotation1953815
version: 1
top level: rbs+crtZ
location: https://synbiohub.org/public/igem/BBa_I742158/annotation1953815/range1953815/1
component: BBa_I742157

https://synbiohub.org/public/igem/BBa_I742158/annotation1953815/range1953815/1
type: Range
persistent identity: range1953815
display id: range1953815
version: 1
top level: rbs+crtZ
direction: direction/reverse
start: 17
end: 547
orientation: inline

BBa_K098995
type: ComponentDefinition
persistent identity: BBa K098995
display id: BBa_K098995
version: 1
was generated by: iGEM to SBOL conversion
description: heat sensitive cI QPI with high promoter
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:41Z
mutable provenance: PGW7 plasmid and various BioBricks parts.
owned by: james
owned by: myers
mutable description: This is a heat sensitive cI QPI with a high promoter preceding the repressor (BBa_J23114). It can be used to construct a heat-inducible system if a reporter or indicator is adding to the right of this part.
discontinued: false
dominant: false
experience: experience/Works
group u list: _196_
m user id: 0
owner id: 3394
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: This is a mutagenized form because the original product had an extra PstI site between the repressor and terminator. While there are still extra basepairs between the end of the repressor sequence and the Biobricks scar, they no longer code for a PstI site. The extra base pairs are: [repressor coding region] CTCCAGCGGCCGC [Biobricks scar]
star: true
creator: Harvard iGEM 08
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J23114
component: BBa_S04137
sequence annotation: BBa_J23114
sequence annotation: BBa_S04137
sequence: https://synbiohub.org/public/igem/BBa_K098995_sequence/1

BBa_J23114
type: Component
persistent identity: component2224132
display id: component2224132
version: 1
top level: BBa_K098995
definition: BBa_J23114
access: public

BBa_S04137
type: Component
persistent identity: component2224150
display id: component2224150
version: 1
top level: BBa_K098995
definition: BBa_S04137
access: public

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2224132
display id: annotation2224132
version: 1
top level: BBa_K098995
location: https://synbiohub.org/public/igem/BBa_K098995/annotation2224132/range2224132/1
component: BBa_J23114

https://synbiohub.org/public/igem/BBa_K098995/annotation2224132/range2224132/1
type: Range
persistent identity: range2224132
display id: range2224132
version: 1
top level: BBa_K098995
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_S04137
type: SequenceAnnotation
persistent identity: annotation2224150
display id: annotation2224150
version: 1
top level: BBa_K098995
location: https://synbiohub.org/public/igem/BBa_K098995/annotation2224150/range2224150/1
component: BBa_S04137

https://synbiohub.org/public/igem/BBa_K098995/annotation2224150/range2224150/1
type: Range
persistent identity: range2224150
display id: range2224150
version: 1
top level: BBa_K098995
direction: direction/reverse
start: 44
end: 929
orientation: inline

BBa_J61048
type: ComponentDefinition
persistent identity: BBa J61048
display id: BBa_J61048
version: 1
was generated by: iGEM to SBOL conversion
description: [rnpB-T1] Terminator
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: bob
owned by: james
owned by: myers
mutable description: bob
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: bob
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_J61048_sequence/1

BBa_I742156
type: ComponentDefinition
persistent identity: BBa I742156
display id: BBa_I742156
version: 1
was generated by: iGEM to SBOL conversion
description: crtZ native rbs
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
mutable description: Native ribosome binding site for crtZ (beta-carotene hydroxylase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: A virtual part to be added to the coding sequence blunt (without a scar).
star: false
creator: Chris French
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_I742156_sequence/1

BBa_I742157
type: ComponentDefinition
persistent identity: BBa I742157
display id: BBa_I742157
version: 1
was generated by: iGEM to SBOL conversion
description: crtZ (beta-carotene hydroxylase) coding sequence
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
mutable description: Coding sequence for crtZ (beta-carotene hydroxylase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis pathway. Converts beta-carotene to the yellow pigment zeaxanthin.
discontinued: false
dominant: true
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No special considerations.
star: false
creator: Chris French
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: crtZ
sequence: https://synbiohub.org/public/igem/BBa_I742157_sequence/1

crtZ
type: SequenceAnnotation
persistent identity: annotation1953812
display id: annotation1953812
version: 1
top level: BBa_I742157
location: https://synbiohub.org/public/igem/BBa_I742157/annotation1953812/range1953812/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I742157/annotation1953812/range1953812/1
type: Range
persistent identity: range1953812
display id: range1953812
version: 1
top level: BBa_I742157
direction: direction/forward
start: 1
end: 528
orientation: inline

BBa_J23114
type: ComponentDefinition
persistent identity: BBa J23114
display id: BBa_J23114
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23114_sequence/1

BBa_S04137
type: ComponentDefinition
persistent identity: BBa S04137
display id: BBa_S04137
version: 1
was generated by: iGEM to SBOL conversion
description: heat sensitive cI QPI
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:14:35Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _196_
m user id: 0
owner id: 3394
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Harvard iGEM 08
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_S04136
component: BBa_K098998
sequence annotation: BBa_K098998
sequence annotation: BBa_S04136
sequence: https://synbiohub.org/public/igem/BBa_S04137_sequence/1

BBa_S04136
type: Component
persistent identity: component2222210
display id: component2222210
version: 1
top level: BBa_S04137
definition: BBa_S04136
access: public

BBa_K098998
type: Component
persistent identity: component2222198
display id: component2222198
version: 1
top level: BBa_S04137
definition: BBa_K098998
access: public

BBa_K098998
type: SequenceAnnotation
persistent identity: annotation2222198
display id: annotation2222198
version: 1
top level: BBa_S04137
location: https://synbiohub.org/public/igem/BBa_S04137/annotation2222198/range2222198/1
component: BBa_K098998

https://synbiohub.org/public/igem/BBa_S04137/annotation2222198/range2222198/1
type: Range
persistent identity: range2222198
display id: range2222198
version: 1
top level: BBa_S04137
direction: direction/reverse
start: 1
end: 726
orientation: inline

BBa_S04136
type: SequenceAnnotation
persistent identity: annotation2222210
display id: annotation2222210
version: 1
top level: BBa_S04137
location: https://synbiohub.org/public/igem/BBa_S04137/annotation2222210/range2222210/1
component: BBa_S04136

https://synbiohub.org/public/igem/BBa_S04137/annotation2222210/range2222210/1
type: Range
persistent identity: range2222210
display id: range2222210
version: 1
top level: BBa_S04137
direction: direction/reverse
start: 735
end: 886
orientation: inline

BBa_K098998
type: ComponentDefinition
persistent identity: BBa K098998
display id: BBa_K098998
version: 1
was generated by: iGEM to SBOL conversion
description: cIts coding region with rbs
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:41Z
mutable provenance: The source of the part is the PGW7 plasmid.
owned by: james
owned by: myers
mutable description: This is the temperature sensitive cI coding region from the PGW7 plasmid. It was taken from the plasmid via PCR, and a RBS was also added (BBa_B0034). There is no scar between the RBS and cI coding region.
discontinued: false
dominant: false
experience: experience/None
group u list: _196_
m user id: 0
owner id: 3394
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Obtained by PCR. RBS added by PCR.
star: false
creator: Harvard iGEM 08
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K098997
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K098997
sequence: https://synbiohub.org/public/igem/BBa_K098998_sequence/1

BBa_K098997
type: Component
persistent identity: component1988857
display id: component1988857
version: 1
top level: BBa_K098998
definition: BBa_K098997
access: public

BBa_B0034
type: Component
persistent identity: component1988855
display id: component1988855
version: 1
top level: BBa_K098998
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1988855
display id: annotation1988855
version: 1
top level: BBa_K098998
location: https://synbiohub.org/public/igem/BBa_K098998/annotation1988855/range1988855/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K098998/annotation1988855/range1988855/1
type: Range
persistent identity: range1988855
display id: range1988855
version: 1
top level: BBa_K098998
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K098997
type: SequenceAnnotation
persistent identity: annotation1988857
display id: annotation1988857
version: 1
top level: BBa_K098998
location: https://synbiohub.org/public/igem/BBa_K098998/annotation1988857/range1988857/1
component: BBa_K098997

https://synbiohub.org/public/igem/BBa_K098998/annotation1988857/range1988857/1
type: Range
persistent identity: range1988857
display id: range1988857
version: 1
top level: BBa_K098998
direction: direction/reverse
start: 13
end: 726
orientation: inline

BBa_S04136
type: ComponentDefinition
persistent identity: BBa S04136
display id: BBa_S04136
version: 1
was generated by: iGEM to SBOL conversion
description: terminator and cI promoter
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:14:35Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _196_
m user id: 0
owner id: 3394
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Harvard iGEM 08
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_R0051
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence annotation: BBa_R0051
sequence: https://synbiohub.org/public/igem/BBa_S04136_sequence/1

BBa_R0051
type: Component
persistent identity: component1988865
display id: component1988865
version: 1
top level: BBa_S04136
definition: cI lam
access: public

BBa_B0011
type: Component
persistent identity: component1988862
display id: component1988862
version: 1
top level: BBa_S04136
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component1988858
display id: component1988858
version: 1
top level: BBa_S04136
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1988858
display id: annotation1988858
version: 1
top level: BBa_S04136
location: https://synbiohub.org/public/igem/BBa_S04136/annotation1988858/range1988858/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S04136/annotation1988858/range1988858/1
type: Range
persistent identity: range1988858
display id: range1988858
version: 1
top level: BBa_S04136
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation1988862
display id: annotation1988862
version: 1
top level: BBa_S04136
location: https://synbiohub.org/public/igem/BBa_S04136/annotation1988862/range1988862/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_S04136/annotation1988862/range1988862/1
type: Range
persistent identity: range1988862
display id: range1988862
version: 1
top level: BBa_S04136
direction: direction/forward
start: 50
end: 95
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1988865
display id: annotation1988865
version: 1
top level: BBa_S04136
location: https://synbiohub.org/public/igem/BBa_S04136/annotation1988865/range1988865/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_S04136/annotation1988865/range1988865/1
type: Range
persistent identity: range1988865
display id: range1988865
version: 1
top level: BBa_S04136
direction: direction/forward
start: 104
end: 152
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

BBa_K098997
type: ComponentDefinition
persistent identity: BBa K098997
display id: BBa_K098997
version: 1
was generated by: iGEM to SBOL conversion
description: cIts coding region
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:41Z
mutable provenance: It comes from PGW7 plasmid.
owned by: james
owned by: myers
mutable description: This it the temperature sensitive cI from the PGW7 plasmid. It has the Elowitz RBS in front (BBa_B0034). It produces a temperature sensitive cI repressor that loses binding efficiency with the cI promoter between 35 and 42 degrees C.
discontinued: false
dominant: false
experience: experience/None
group u list: _196_
m user id: 0
owner id: 3394
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Obtained by PCR. RBS also added by PCR.
star: false
creator: Harvard iGEM 08
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cIts
sequence: https://synbiohub.org/public/igem/BBa_K098997_sequence/1

cIts
type: SequenceAnnotation
persistent identity: annotation1988780
display id: annotation1988780
version: 1
top level: BBa_K098997
location: https://synbiohub.org/public/igem/BBa_K098997/annotation1988780/range1988780/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K098997/annotation1988780/range1988780/1
type: Range
persistent identity: range1988780
display id: range1988780
version: 1
top level: BBa_K098997
direction: direction/forward
start: 1
end: 714
orientation: inline

https://synbiohub.org/public/igem/BBa_K539281_sequence/1
type: Sequence
persistent identity: BBa K539281 sequence
display id: BBa_K539281_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttatggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaaatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagctctaccggagaaattatgttgtggatttggaatgccctgatcgttttcgttaccgtgattggcatggaagtgattgctgcactggcacacaaatacatcatgcacggctggggttggggatggcatctttcacatcatgaaccgcgtaaaggtgcgtttgaagttaacgatctttatgccgtggtttttgctgcattatcgatcctgctgatttatctgggcagtacaggaatgtggccgctccagtggattggcgcaggtatgacggcgtatggattactctattttatggtgcacgacgggctggtgcatcaacgttggccattccgctatattccacgcaagggctacctcaaacggttgtatatggcgcaccgtatgcatcacgccgtcaggggcaaagaaggttgtgtttcttttggcttcctctatgcgccgcccctgtcaaaacttcaggcgacgctccgggaaagacatggcgctagagcgggcgctgccagagatgcgcagggcggggaggatgagcccgcatccgggaagtaataatactagagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742158_sequence/1
type: Sequence
persistent identity: BBa I742158 sequence
display id: BBa_I742158_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctctaccggagaaattatgttgtggatttggaatgccctgatcgttttcgttaccgtgattggcatggaagtgattgctgcactggcacacaaatacatcatgcacggctggggttggggatggcatctttcacatcatgaaccgcgtaaaggtgcgtttgaagttaacgatctttatgccgtggtttttgctgcattatcgatcctgctgatttatctgggcagtacaggaatgtggccgctccagtggattggcgcaggtatgacggcgtatggattactctattttatggtgcacgacgggctggtgcatcaacgttggccattccgctatattccacgcaagggctacctcaaacggttgtatatggcgcaccgtatgcatcacgccgtcaggggcaaagaaggttgtgtttcttttggcttcctctatgcgccgcccctgtcaaaacttcaggcgacgctccgggaaagacatggcgctagagcgggcgctgccagagatgcgcagggcggggaggatgagcccgcatccgggaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K098995_sequence/1
type: Sequence
persistent identity: BBa K098995 sequence
display id: BBa_K098995_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttatggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaaatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61048_sequence/1
type: Sequence
persistent identity: BBa J61048 sequence
display id: BBa_J61048_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742156_sequence/1
type: Sequence
persistent identity: BBa I742156 sequence
display id: BBa_I742156_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctctaccggagaaatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742157_sequence/1
type: Sequence
persistent identity: BBa I742157 sequence
display id: BBa_I742157_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttgtggatttggaatgccctgatcgttttcgttaccgtgattggcatggaagtgattgctgcactggcacacaaatacatcatgcacggctggggttggggatggcatctttcacatcatgaaccgcgtaaaggtgcgtttgaagttaacgatctttatgccgtggtttttgctgcattatcgatcctgctgatttatctgggcagtacaggaatgtggccgctccagtggattggcgcaggtatgacggcgtatggattactctattttatggtgcacgacgggctggtgcatcaacgttggccattccgctatattccacgcaagggctacctcaaacggttgtatatggcgcaccgtatgcatcacgccgtcaggggcaaagaaggttgtgtttcttttggcttcctctatgcgccgcccctgtcaaaacttcaggcgacgctccgggaaagacatggcgctagagcgggcgctgccagagatgcgcagggcggggaggatgagcccgcatccgggaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23114_sequence/1
type: Sequence
persistent identity: BBa J23114 sequence
display id: BBa_J23114_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttatggctagctcagtcctaggtacaatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04137_sequence/1
type: Sequence
persistent identity: BBa S04137 sequence
display id: BBa_S04137_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaaatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K098998_sequence/1
type: Sequence
persistent identity: BBa K098998 sequence
display id: BBa_K098998_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaaatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04136_sequence/1
type: Sequence
persistent identity: BBa S04136 sequence
display id: BBa_S04136_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K098997_sequence/1
type: Sequence
persistent identity: BBa K098997 sequence
display id: BBa_K098997_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z