LOCUS BBa_K539392 6153 bp DNA linear 25-Nov-2024 DEFINITION Vio operon ABDE(each preceded by their own ribosome binding site) with a terminator ACCESSION BBa_K539392 VERSION BBa_K539392.1 FEATURES Location/Qualifiers RBS 1..17 /label=rbs CDS 18..1274 /label=vioA misc_feature 1275..1291 /label=misc CDS 1292..4294 /label=vioB RBS 4295..4312 /label=rbs CDS 4313..5439 /label=vioD RBS 5440..5456 /label=rbs CDS 5457..6032 /label=vioE misc_feature 1..6032 /label=BBa_K274003 terminator 6041..6153 /label=BBa_J61048 ORIGIN 1 ttaaggaggt aaaaaaaatg aaacattctt ccgatatctg cattgttggt gctggtattt 61 ctggtttgac gtgcgcaagc catctgctgg acagcccggc atgccgtggt ctgagcctgc 121 gtatctttga catgcagcaa gaagccggtg gccgtatccg cagcaaaatg ctggatggta 181 aggcaagcat tgaactgggc gcaggtcgct actcccctca gttgcacccg catttccaaa 241 gcgcaatgca gcactatagc caaaagagcg aagtctatcc gttcacccag ttgaagttca 301 aatctcacgt gcagcaaaag ctgaagcgcg ccatgaatga actgtccccg cgtctgaaag 361 agcatggtaa agagagcttt ttgcagtttg tcagccgtta tcaaggtcac gatagcgcgg 421 ttggtatgat ccgctctatg ggttacgacg cactgttcct gccggatatc agcgcagaaa 481 tggcctacga cattgtgggt aagcacccgg agatccagag cgtgacggac aacgacgcga 541 accaatggtt tgcagcggaa acgggctttg ctggtctgat tcagggcatc aaggctaagg 601 ttaaggcggc aggtgcgcgt tttagcctgg gttatcgtct gctgagcgtc cgtaccgacg 661 gtgacggcta cctgctgcaa ctggcaggtg acgacggctg gaaactggag caccgtaccc 721 gccatctgat tctggcgatt ccgccgagcg cgatggcggg tttgaatgtt gattttccag 781 aagcctggtc cggtgcgcgc tatggcagcc tgccgctgtt taagggcttt ctgacgtacg 841 gtgagccgtg gtggttggac tacaaactgg acgatcaggt gctgattgtt gacaacccgc 901 tgcgcaaaat ctatttcaaa ggcgataagt acctgttctt ctataccgat agcgagatgg 961 cgaattactg gcgcggttgt gtcgcggagg gcgaggacgg ttacctggag caaattcgca 1021 cccatttggc tagcgcactg ggtatcgtcc gtgaacgtat cccgcaaccg ctggcacacg 1081 ttcacaagta ttgggcgcac ggcgttgagt tttgccgtga ttctgatatt gaccacccga 1141 gcgcactgtc tcatcgcgac agcggtatca tcgcgtgctc cgatgcgtac acggagcatt 1201 gtggttggat ggagggcggt ctgctgagcg cccgtgaggc aagccgtctg ctgttgcagc 1261 gtatcgccgc gtgattaagg aggtaaaaaa aatgagcatt ctggatttcc cgcgtatcca 1321 cttccgtggc tgggcccgtg tcaatgcgcc gaccgcgaac cgcgatccgc acggccacat 1381 cgatatggcc agcaataccg tggcgatggc gggtgagccg ttcgacctgg cacgccatcc 1441 tacggagttc caccgtcacc tgcgctccct gggtccgcgc ttcggcttgg atggtcgtgc 1501 tgacccggaa ggcccgttca gcctggccga gggctacaac gctgccggta acaaccactt 1561 ttcgtgggag agcgcaaccg ttagccacgt gcaatgggat ggcggtgagg cggatcgtgg 1621 tgacggtctg gtcggtgctc gtttggcact gtggggtcac tacaatgatt atctgcgtac 1681 caccttcaat cgtgctcgtt gggtcgacag cgacccgacg cgccgtgacg ctgcacaaat 1741 ctatgcgggc caattcacca ttagcccggc tggtgccggt ccgggtacgc cgtggctgtt 1801 tacggcagac attgatgata gccatggtgc acgttggacg cgtggcggcc acattgcaga 1861 gcgtggcggc cacttcttgg atgaagagtt tggtctggca cgcctgtttc agttctctgt 1921 gccgaaagat cacccacatt ttctgtttca cccgggtccg tttgattccg aggcctggcg 1981 tcgtctgcaa ttggctctgg aggatgacga cgttctgggt ctgaccgtgc aatatgcgtt 2041 gttcaatatg agcaccccgc ctcagccgaa cagcccggtt tttcacgata tggtcggtgt 2101 tgtcggtctg tggcgtcgtg gtgaactggc gagctacccg gctggtcgtc tgctgcgtcc 2161 gcgtcaaccg ggtctgggtg acctgaccct gcgcgtcaac ggtggtcgcg ttgcgctgaa 2221 tttggcgtgt gccattccgt tcagcactcg tgccgcgcag ccaagcgcac cggaccgcct 2281 gaccccggac ctgggtgcca aactgccgct gggcgatctg ctgctgcgtg atgaggacgg 2341 cgcactgttg gcacgtgtgc cgcaggctct gtaccaagac tattggacga atcacggtat 2401 tgtggacctg ccgctgctgc gcgaaccgcg tggtagcttg accctgagca gcgaactggc 2461 ggagtggcgt gagcaagact gggtcaccca aagcgacgcg tctaacctgt acctggaggc 2521 accggatcgc cgtcacggtc gctttttccc tgagagcatc gcgctgcgca gctactttcg 2581 cggtgaagcg cgtgcgcgtc cggatatccc gcatcgtatc gagggcatgg gcctggtcgg 2641 cgtcgaatct cgtcaggatg gcgacgctgc ggaatggcgt ctgacgggtc tgcgtccggg 2701 tccggcacgc attgttctgg acgatggtgc cgaggcgatc cctctgcgtg ttctgcctga 2761 cgattgggcg ctggatgacg cgaccgtcga agaagtggat tacgcctttt tgtaccgcca 2821 cgttatggcg tattacgagc tggtgtatcc attcatgagc gacaaggtgt tttccctggc 2881 tgatcgttgc aaatgtgaaa cgtacgcacg tctgatgtgg cagatgtgtg atccgcagaa 2941 ccgcaacaag tcctattaca tgccgagcac ccgcgaactg tcggcaccga aagctcgttt 3001 gttcttgaag tatctggccc acgtggaagg ccaggcacgc ctgcaagcac ctccgccagc 3061 gggtccggca cgcattgaat ctaaagccca gttggcggca gagctgcgta aagccgtcga 3121 cctggagctg tctgtgatgc tgcaatacct gtacgcggcg tatagcattc cgaactatgc 3181 acagggccaa caacgtgttc gtgacggtgc gtggaccgcc gagcagctgc aactggcgtg 3241 cggtagcggt gaccgtcgcc gtgatggcgg tattcgtgca gcactgctgg aaattgctca 3301 tgaagaaatg attcattacc tggtcgttaa caacctgctg atggccctgg gcgagccgtt 3361 ctacgcgggt gtcccgctga tgggcgaagc ggcacgtcag gcgtttggcc tggacaccga 3421 gttcgctctg gaaccgttta gcgaaagcac gctggcacgt tttgttcgtc tggaatggcc 3481 gcactttatc ccagcaccgg gcaaatccat cgcggactgc tatgccgcca ttcgtcaggc 3541 gtttttggat ctgccggact tgtttggtgg cgaggcaggt aagcgtggcg gtgaacacca 3601 cctgttcctg aatgagctga ccaaccgtgc gcatccgggt tatcaactgg aagttttcga 3661 tcgcgactcg gcgctgtttg gtattgcatt tgtgaccgat cagggcgaag gtggcgctct 3721 ggacagcccg cactacgaac atagccattt tcaacgtctg cgtgaaatga gcgcgcgtat 3781 catggctcaa agcgcaccgt tcgaaccggc gctgccggcg ttgcgtaatc cggttctgga 3841 tgagagcccg ggttgccaac gtgtcgcaga cggtcgtgcg cgtgcgctga tggcattgta 3901 ccaaggcgtt tatgagctga tgtttgcgat gatggcgcag cacttcgccg tgaaaccgct 3961 gggtagcttg cgtcgcagcc gcctgatgaa cgcagcaatc gatctgatga ccggtctgtt 4021 gcgtccgctg agctgcgcgc tgatgaacct gccaagcggc atcgccggtc gcacggccgg 4081 tccgccgctg ccgggtccgg ttgacacccg tagctatgac gactacgcgc tgggctgtcg 4141 catgctggca cgccgttgcg agcgtctgct ggagcaggcg agcatgctgg aaccgggttg 4201 gctgccggat gcgcagatgg agctgctgga tttctatcgt cgccaaatgc tggacttggc 4261 gtgcggcaaa ctgagccgcg aggcctaagg atctttaagg aggtaaaaaa aatgaagatt 4321 ctggtcattg gtgctggtcc agctggtctg gttttcgcat cccaactgaa gcaggcacgc 4381 cctttgtggg ccattgacat cgtggagaag aatgacgagc aagaagtgct gggctggggt 4441 gtcgtgctgc ctggccgtcc gggtcagcac ccggcgaacc cgctgtccta tctggatgca 4501 ccggagcgtc tgaatccgca atttctggag gacttcaaac tggtgcatca taatgagccg 4561 tccttgatgt ccacgggcgt tttgttgtgc ggcgtggagc gtcgcggtct ggttcacgcg 4621 ctgcgcgata agtgccgcag ccaaggcatt gctattcgtt tcgaaagccc gttgctggaa 4681 cacggtgagc tgccgctggc ggactatgat ctggtggtcc tggctaatgg tgttaatcac 4741 aaaaccgcgc atttcaccga ggctctggtc ccgcaggtgg actacggccg caataagtac 4801 atttggtatg gcactagcca gctgttcgat cagatgaatc tggtttttcg tacccatggt 4861 aaagatatct ttatcgcgca tgcctataag tatagcgata ccatgagcac gttcattgtc 4921 gaatgtagcg aagagactta cgcacgcgca cgcctgggcg aaatgtccga agaggcgagc 4981 gcagaatacg ttgctaaggt gttccaggcc gagctgggtg gtcacggcct ggtgagccag 5041 ccgggtctgg gttggcgtaa cttcatgacg ttgtctcatg accgttgtca tgatggtaag 5101 ttggttctgc tgggtgacgc gctgcaaagc ggtcacttta gcatcggcca cggcaccacg 5161 atggccgtgg tggtggcgca gctgctggtt aaagcgctgt gtaccgaaga tggtgtgcct 5221 gccgcgctga aacgtttcga agagcgtgcc ctgccgctgg tgcagttgtt ccgtggccac 5281 gcagacaaca gccgcgtttg gttcgaaacc gtcgaagagc gcatgcacct gtcctcggcg 5341 gaatttgtgc aaagcttcga cgcacgccgc aaaagcctgc cgccgatgcc ggaagcactg 5401 gcgcagaatc tgcgttatgc tttgcagcgc tgatgatcat taaggaggta aaaaaaatgg 5461 agaaccgtga gccaccactg ttgccagccc gttggagcag cgcctatgtc tcttattgga 5521 gcccgatgct gccggatgac cagctgacca gcggctattg ctggttcgac tatgaacgtg 5581 acatctgtcg tattgacggc ctgttcaatc cgtggagcga gcgtgatact ggttatcgcc 5641 tgtggatgtc ggaggttggt aatgcggcca gcggccgtac ctggaaacaa aaagtcgcct 5701 atggtcgtga gcgtaccgcc ctgggtgaac agctgtgtga gcgtccgctg gatgatgaga 5761 ctggcccttt tgccgaattg ttcctgccac gcgatgtcct gcgccgtctg ggtgcccgtc 5821 acattggccg tcgcgtggtt ctgggtcgcg aagcggacgg ttggcgttac cagcgcccag 5881 gtaaaggtcc gagcaccctg tacctggatg cggcgagcgg cactccactg cgcatggtca 5941 ccggcgatga agcgtcgcgt gcaagcctgc gtgattttcc gaatgtgagc gaggcggaga 6001 tcccggacgc ggttttcgcg gccaagcgct aatactagag ccggcttatc ggtcagtttc 6061 acctgattta cgtaaaaacc cgcttcggcg ggtttttgct tttggagggg cagaaagatg 6121 aatgactgtc cacgacgcta tacccaaaag aaa //