LOCUS       BBa_K539421              260 bp    DNA     linear       17-May-2024
DEFINITION  promoter (lacI regulated)+RNA thermometer
ACCESSION   BBa_K539421
VERSION     BBa_K539421.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             255..260
                     /label=rbs
     misc_feature    231..236
                     /label=scar adaptation
     stem_loop       209..226
                     /label=stem_loop
     stem_loop       232..260
                     /label=predicted stem-loop extending to one position 
                     before start codon
     misc_feature    209..260
                     /label=BBa_K115002
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagaggg acaagcaatg cttgccttga atagtaactt
      241 ttgaatagtg attcaggagg
//