LOCUS       BBa_K539461             2232 bp    DNA     linear       25-Nov-2024
DEFINITION  promoter (lacI regulated)+RNA thermometer+vioD with RNA 
            thermometer+tetR+double terminator
ACCESSION   BBa_K539461
VERSION     BBa_K539461.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             255..260
                     /label=rbs
     misc_feature    231..236
                     /label=scar adaptation
     stem_loop       209..226
                     /label=stem_loop
     stem_loop       232..260
                     /label=predicted stem-loop extending to one position 
                     before start codon
     misc_feature    209..260
                     /label=BBa_K115002
     CDS             267..1360
                     /label=vioD
     CDS             267..1360
                     /label=BBa_K539413
     RBS             1415..1420
                     /label=rbs
     misc_feature    1391..1396
                     /label=scar adaptation
     stem_loop       1369..1386
                     /label=stem_loop
     stem_loop       1392..1420
                     /label=predicted stem-loop extending to one position 
                     before start codon
     misc_feature    1369..1420
                     /label=BBa_K115002
     CDS             1430..2046
                     /label=tetR
     misc_feature    2047..2080
                     /label=SsrA
     misc_feature    2087..2111
                     /label=Help:Barcodes
     CDS             1427..2111
                     /label=BBa_C0040
     terminator      2120..2232
                     /label=BBa_J61048
     misc_feature    1369..2232
                     /label=BBa_K332033
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagaggg acaagcaatg cttgccttga atagtaactt
      241 ttgaatagtg attcaggagg tactagatga agattctggt cattggtgct ggtccagctg
      301 gtctggtttt cgcatcccaa ctgaagcagg cacgcccttt gtgggccatt gacatcgtgg
      361 agaagaatga cgagcaagaa gtgctgggct ggggtgtcgt gctgcctggc cgtccgggtc
      421 agcacccggc gaacccgctg tcctatctgg atgcaccgga gcgtctgaat ccgcaatttc
      481 tggaggactt caaactggtg catcataatg agccgtcctt gatgtccacg ggcgttttgt
      541 tgtgcggcgt ggagcgtcgc ggtctggttc acgcgctgcg cgataagtgc cgcagccaag
      601 gcattgctat tcgtttcgaa agcccgttgc tggaacacgg tgagctgccg ctggcggact
      661 atgatctggt ggtcctggct aatggtgtta atcacaaaac cgcgcatttc accgaggctc
      721 tggtcccgca ggtggactac ggccgcaata agtacatttg gtatggcact agccagctgt
      781 tcgatcagat gaatctggtt tttcgtaccc atggtaaaga tatctttatc gcgcatgcct
      841 ataagtatag cgataccatg agcacgttca ttgtcgaatg tagcgaagag acttacgcac
      901 gcgcacgcct gggcgaaatg tccgaagagg cgagcgcaga atacgttgct aaggtgttcc
      961 aggccgagct gggtggtcac ggcctggtga gccagccggg tctgggttgg cgtaacttca
     1021 tgacgttgtc tcatgaccgt tgtcatgatg gtaagttggt tctgctgggt gacgcgctgc
     1081 aaagcggtca ctttagcatc ggccacggca ccacgatggc cgtggtggtg gcgcagctgc
     1141 tggttaaagc gctgtgtacc gaagatggtg tgcctgccgc gctgaaacgt ttcgaagagc
     1201 gtgccctgcc gctggtgcag ttgttccgtg gccacgcaga caacagccgc gtttggttcg
     1261 aaaccgtcga agagcgcatg cacctgtcct cggcggaatt tgtgcaaagc ttcgacgcac
     1321 gccgcaaaag cctgccgccg atgccggaag cactggcgca tactagaggg acaagcaatg
     1381 cttgccttga atagtaactt ttgaatagtg attcaggagg tactagatgt ccagattaga
     1441 taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt
     1501 aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt
     1561 aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac
     1621 tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag
     1681 ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc
     1741 tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt
     1801 ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt
     1861 attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag
     1921 tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc
     1981 cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag
     2041 tgggtccgct gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta
     2101 gtgtagatca ctactagagc cggcttatcg gtcagtttca cctgatttac gtaaaaaccc
     2161 gcttcggcgg gtttttgctt ttggaggggc agaaagatga atgactgtcc acgacgctat
     2221 acccaaaaga aa
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