##gff-version 3
##sequence-region BBa_K539461 1 2232
BBa_K539461	.	CDS	267	1360	.	+	0	ID=BBa_K539413;Name=BBa_K539413
BBa_K539461	.	CDS	267	1360	.	+	0	ID=annotation2138792;Name=vioD;Parent=BBa_K539413
BBa_K539461	.	engineered_region	1369	2232	.	+	0	ID=BBa_K332033;Name=BBa_K332033
BBa_K539461	.	mature_transcript_region	1369	1420	.	+	0	ID=BBa_K115002;Name=BBa_K115002;Parent=BBa_K332033
BBa_K539461	.	ribosome_entry_site	1415	1420	.	+	0	ID=annotation1966897;Name=rbs;Parent=BBa_K115002
BBa_K539461	.	stem_loop	1392	1420	.	+	0	ID=annotation1966931;Name=predicted stem-loop extending to one position before start codon;Parent=BBa_K115002
BBa_K539461	.	stem_loop	1369	1386	.	+	0	ID=annotation1966930;Name=stem_loop;Parent=BBa_K115002
BBa_K539461	.	sequence_alteration	1391	1396	.	+	0	ID=annotation1966899;Name=scar adaptation;Parent=BBa_K115002
BBa_K539461	.	CDS	1427	2111	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K332033
BBa_K539461	.	engineered_tag	2087	2111	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K539461	.	CDS	1430	2046	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K539461	.	tag	2047	2080	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K539461	.	terminator	2120	2232	.	+	0	ID=BBa_J61048;Name=BBa_J61048;Parent=BBa_K332033
BBa_K539461	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K539461	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K539461	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K539461	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K539461	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K539461	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K539461	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K539461	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K539461	.	mature_transcript_region	209	260	.	+	0	ID=BBa_K115002;Name=BBa_K115002
BBa_K539461	.	ribosome_entry_site	255	260	.	+	0	ID=annotation1966897;Name=rbs;Parent=BBa_K115002
BBa_K539461	.	stem_loop	232	260	.	+	0	ID=annotation1966931;Name=predicted stem-loop extending to one position before start codon;Parent=BBa_K115002
BBa_K539461	.	stem_loop	209	226	.	+	0	ID=annotation1966930;Name=stem_loop;Parent=BBa_K115002
BBa_K539461	.	sequence_alteration	231	236	.	+	0	ID=annotation1966899;Name=scar adaptation;Parent=BBa_K115002
>BBa_K539461
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggacaagcaatgcttgccttgaatagtaa
cttttgaatagtgattcaggaggtactagatgaagattctggtcattggtgctggtccagctggtctggttttcgcatc
ccaactgaagcaggcacgccctttgtgggccattgacatcgtggagaagaatgacgagcaagaagtgctgggctggggt
gtcgtgctgcctggccgtccgggtcagcacccggcgaacccgctgtcctatctggatgcaccggagcgtctgaatccgc
aatttctggaggacttcaaactggtgcatcataatgagccgtccttgatgtccacgggcgttttgttgtgcggcgtgga
gcgtcgcggtctggttcacgcgctgcgcgataagtgccgcagccaaggcattgctattcgtttcgaaagcccgttgctg
gaacacggtgagctgccgctggcggactatgatctggtggtcctggctaatggtgttaatcacaaaaccgcgcatttca
ccgaggctctggtcccgcaggtggactacggccgcaataagtacatttggtatggcactagccagctgttcgatcagat
gaatctggtttttcgtacccatggtaaagatatctttatcgcgcatgcctataagtatagcgataccatgagcacgttc
attgtcgaatgtagcgaagagacttacgcacgcgcacgcctgggcgaaatgtccgaagaggcgagcgcagaatacgttg
ctaaggtgttccaggccgagctgggtggtcacggcctggtgagccagccgggtctgggttggcgtaacttcatgacgtt
gtctcatgaccgttgtcatgatggtaagttggttctgctgggtgacgcgctgcaaagcggtcactttagcatcggccac
ggcaccacgatggccgtggtggtggcgcagctgctggttaaagcgctgtgtaccgaagatggtgtgcctgccgcgctga
aacgtttcgaagagcgtgccctgccgctggtgcagttgttccgtggccacgcagacaacagccgcgtttggttcgaaac
cgtcgaagagcgcatgcacctgtcctcggcggaatttgtgcaaagcttcgacgcacgccgcaaaagcctgccgccgatg
ccggaagcactggcgcatactagagggacaagcaatgcttgccttgaatagtaacttttgaatagtgattcaggaggta
ctagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggttta
acaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgc
tcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattt
tttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacgg
cctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcat
tatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaaga
aagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagag
ccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaa
acgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccggcttatcggtc
agtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccac
gacgctatacccaaaagaaa