BBa_C0040 1 tetR tetracycline repressor from transposon Tn10 (+LVA) 2003-01-31T12:00:00Z 2015-08-31T04:07:23Z Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999) Released HQ 2013 Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.</P> false true _1_ 0 24 7 In stock false References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 </p> <P> References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 </p> <P>BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA. <P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman. annotation23330 1 SsrA range23330 1 621 654 annotation2213989 1 Help:Barcodes range2213989 1 661 685 annotation23329 1 tetR range23329 1 4 620 BBa_K332033 1 BBa_K332033 37&#8451; induced RBS + tetR + double terminator 2010-10-23T11:00:00Z 2015-05-08T01:12:05Z the 37&#8451; induced RBS cames from iGEM08_TUDelft It is a transcriptional unit including 37&#8451; induced RBS + tetR + double terminator. It can be added various promoter to adapt your conditions. false false _452_ 0 7059 9 It's complicated false To regulate following genes by temperature. To maintain cells at 37?? C &#8593; : the tetR protein be generated and repress Ptet's circuit. To release cells at 37?? C &#8595; : the tetR protein not be generated and do not repress Ptet's circuit. false KUO, CHIA-CHUN component2247611 1 BBa_K115002 component2247616 1 BBa_J61048 component2247615 1 BBa_C0040 annotation2247611 1 BBa_K115002 range2247611 1 1 52 annotation2247616 1 BBa_J61048 range2247616 1 752 864 annotation2247615 1 BBa_C0040 range2247615 1 59 743 BBa_K539461 1 BBa_K539461 promoter (lacI regulated)+RNA thermometer+vioD with RNA thermometer+tetR+double terminator 2011-09-26T11:00:00Z 2015-05-08T01:12:37Z a a false false _705_ 0 10239 9 In stock true a false Shu-Han Chang component2264259 1 BBa_K539413 component2264270 1 BBa_K332033 component2264246 1 BBa_R0010 component2264257 1 BBa_K115002 annotation2264257 1 BBa_K115002 range2264257 1 209 260 annotation2264246 1 BBa_R0010 range2264246 1 1 200 annotation2264259 1 BBa_K539413 range2264259 1 267 1360 annotation2264270 1 BBa_K332033 range2264270 1 1369 2232 BBa_J61048 1 BBa_J61048 [rnpB-T1] Terminator 2007-02-20T12:00:00Z 2015-08-31T02:03:00Z bob bob false false _95_ 0 483 95 In stock false bob true John Anderson BBa_K115002 1 BBa_K115002 RNA thermometer (FourU) 2008-07-14T11:00:00Z 2015-05-08T01:09:27Z Salmonella Enterica Tyhpy (CP000886.1) Released HQ 2013 Thermo sensitive small RNA (ROSE structure) which can be used as a RNA regulator. false false _223_ 0 3006 9 In stock true Part of the sequence is altered because of the scar... true Bastiaan van den Berg annotation1966899 1 scar adaptation range1966899 1 23 28 annotation1966897 1 rbs range1966897 1 47 52 annotation1966930 1 stem_loop range1966930 1 1 18 annotation1966931 1 predicted stem-loop extending to one position before start codon range1966931 1 24 52 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961224 1 -35 range1961224 1 137 142 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961225 1 -10 range1961225 1 161 166 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961227 1 start range1961227 1 173 173 annotation1961223 1 CAP binding site range1961223 1 89 126 BBa_K539413 1 BBa_K539413 vioD 2011-09-23T11:00:00Z 2015-05-08T01:12:37Z 2010 iGEM Slovenia Cloned from vioABDE .It catalyze protodeoxyviolaceinic acid(PVA) into protoviolaceinic acid. false false _705_ 0 10239 9 Not in stock false no false Shu-Han Chang annotation2138792 1 vioD range2138792 1 1 1094 BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K332033_sequence 1 ggacaagcaatgcttgccttgaatagtaacttttgaatagtgattcaggaggtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa BBa_K539413_sequence 1 atgaagattctggtcattggtgctggtccagctggtctggttttcgcatcccaactgaagcaggcacgccctttgtgggccattgacatcgtggagaagaatgacgagcaagaagtgctgggctggggtgtcgtgctgcctggccgtccgggtcagcacccggcgaacccgctgtcctatctggatgcaccggagcgtctgaatccgcaatttctggaggacttcaaactggtgcatcataatgagccgtccttgatgtccacgggcgttttgttgtgcggcgtggagcgtcgcggtctggttcacgcgctgcgcgataagtgccgcagccaaggcattgctattcgtttcgaaagcccgttgctggaacacggtgagctgccgctggcggactatgatctggtggtcctggctaatggtgttaatcacaaaaccgcgcatttcaccgaggctctggtcccgcaggtggactacggccgcaataagtacatttggtatggcactagccagctgttcgatcagatgaatctggtttttcgtacccatggtaaagatatctttatcgcgcatgcctataagtatagcgataccatgagcacgttcattgtcgaatgtagcgaagagacttacgcacgcgcacgcctgggcgaaatgtccgaagaggcgagcgcagaatacgttgctaaggtgttccaggccgagctgggtggtcacggcctggtgagccagccgggtctgggttggcgtaacttcatgacgttgtctcatgaccgttgtcatgatggtaagttggttctgctgggtgacgcgctgcaaagcggtcactttagcatcggccacggcaccacgatggccgtggtggtggcgcagctgctggttaaagcgctgtgtaccgaagatggtgtgcctgccgcgctgaaacgtttcgaagagcgtgccctgccgctggtgcagttgttccgtggccacgcagacaacagccgcgtttggttcgaaaccgtcgaagagcgcatgcacctgtcctcggcggaatttgtgcaaagcttcgacgcacgccgcaaaagcctgccgccgatgccggaagcactggcgca BBa_J61048_sequence 1 ccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa BBa_C0040_sequence 1 atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac BBa_K539461_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggacaagcaatgcttgccttgaatagtaacttttgaatagtgattcaggaggtactagatgaagattctggtcattggtgctggtccagctggtctggttttcgcatcccaactgaagcaggcacgccctttgtgggccattgacatcgtggagaagaatgacgagcaagaagtgctgggctggggtgtcgtgctgcctggccgtccgggtcagcacccggcgaacccgctgtcctatctggatgcaccggagcgtctgaatccgcaatttctggaggacttcaaactggtgcatcataatgagccgtccttgatgtccacgggcgttttgttgtgcggcgtggagcgtcgcggtctggttcacgcgctgcgcgataagtgccgcagccaaggcattgctattcgtttcgaaagcccgttgctggaacacggtgagctgccgctggcggactatgatctggtggtcctggctaatggtgttaatcacaaaaccgcgcatttcaccgaggctctggtcccgcaggtggactacggccgcaataagtacatttggtatggcactagccagctgttcgatcagatgaatctggtttttcgtacccatggtaaagatatctttatcgcgcatgcctataagtatagcgataccatgagcacgttcattgtcgaatgtagcgaagagacttacgcacgcgcacgcctgggcgaaatgtccgaagaggcgagcgcagaatacgttgctaaggtgttccaggccgagctgggtggtcacggcctggtgagccagccgggtctgggttggcgtaacttcatgacgttgtctcatgaccgttgtcatgatggtaagttggttctgctgggtgacgcgctgcaaagcggtcactttagcatcggccacggcaccacgatggccgtggtggtggcgcagctgctggttaaagcgctgtgtaccgaagatggtgtgcctgccgcgctgaaacgtttcgaagagcgtgccctgccgctggtgcagttgttccgtggccacgcagacaacagccgcgtttggttcgaaaccgtcgaagagcgcatgcacctgtcctcggcggaatttgtgcaaagcttcgacgcacgccgcaaaagcctgccgccgatgccggaagcactggcgcatactagagggacaagcaatgcttgccttgaatagtaacttttgaatagtgattcaggaggtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa BBa_K115002_sequence 1 ggacaagcaatgcttgccttgaatagtaacttttgaatagtgattcaggagg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z