LOCUS       BBa_K539563             2594 bp    DNA     linear       22-Nov-2024
DEFINITION  vioC+a RBS+lacI repressor+RNA thermometer+tetR+double terminator
ACCESSION   BBa_K539563
VERSION     BBa_K539563.1  
FEATURES             Location/Qualifiers
     CDS             1..543
                     /label=vioC
     CDS             1..543
                     /label=BBa_K539513
     misc_feature    556..559
                     /label=conserved
     RBS             552..563
                     /label=BBa_B0034
     misc_feature    1659..1697
                     /label=LVA
     CDS             570..1697
                     /label=lacI-LVA
     misc_feature    570..1697
                     /label=BBa_C0012
     misc_feature    1698..1722
                     /label=Help:Barcodes
     CDS             570..1697
                     /label=BBa_C0012
     RBS             1777..1782
                     /label=rbs
     misc_feature    1753..1758
                     /label=scar adaptation
     stem_loop       1731..1748
                     /label=stem_loop
     stem_loop       1754..1782
                     /label=predicted stem-loop extending to one position 
                     before start codon
     misc_feature    1731..1782
                     /label=BBa_K115002
     CDS             1792..2408
                     /label=tetR
     misc_feature    2409..2442
                     /label=SsrA
     misc_feature    2449..2473
                     /label=Help:Barcodes
     CDS             1789..2473
                     /label=BBa_C0040
     terminator      2482..2594
                     /label=BBa_J61048
     misc_feature    1731..2594
                     /label=BBa_K332033
     misc_feature    552..2594
                     /label=BBa_K539558
ORIGIN
        1 aatgaaacgt gcgattatcg ttggtggcgg cctggcgggt ggcctgaccg cgatctacct
       61 ggcgaagcgt ggctacgaag tgcacgtcgt ggagaagcgt ggtgatcctc tgcgcgatct
      121 gagctcttac gtggacgttg ttagcagccg tgcgatcggc gtgagcatga ccgttcgtgg
      181 tatcaagagc gttttggctg cgggcattcc gcgtgcagag ctggatgcgt gtggcgaacc
      241 gatcgtggca atggctttct ccgtgggtgg tcagtatcgc atgcgcgaac tgaagccgtt
      301 ggaggatttc cgtccgctga gcttgaaccg tgcggcgttt caaaagctgc tgaacaaata
      361 cgcgaacctg gcaggcgttc gttactactt tgagcataag tgcctggatg ttgacctgga
      421 tggtaagagc gtgttgattc agggcaaaga tggtcagccg cagcgtctgc aaggtgacat
      481 gattatcggt gcggatggcg cccacagcgc cgtccgtcag gcgatgcaga gcggcctgcg
      541 tcgtactaga gaaagaggag aaatactaga tggtgaatgt gaaaccagta acgttatacg
      601 atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca
      661 gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca
      721 ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca
      781 cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg
      841 atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta
      901 aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc
      961 tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc
     1021 ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc
     1081 gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc
     1141 cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca
     1201 atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac
     1261 aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc
     1321 agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata
     1381 tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca
     1441 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
     1501 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa
     1561 ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     1621 agctggcacg acaggtttcc cgactggaaa gcgggcaggc tgcaaacgac gaaaactacg
     1681 ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag ggacaagcaa
     1741 tgcttgcctt gaatagtaac ttttgaatag tgattcagga ggtactagat gtccagatta
     1801 gataaaagta aagtgattaa cagcgcatta gagctgctta atgaggtcgg aatcgaaggt
     1861 ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc agcctacatt gtattggcat
     1921 gtaaaaaata agcgggcttt gctcgacgcc ttagccattg agatgttaga taggcaccat
     1981 actcactttt gccctttaga aggggaaagc tggcaagatt ttttacgtaa taacgctaaa
     2041 agttttagat gtgctttact aagtcatcgc gatggagcaa aagtacattt aggtacacgg
     2101 cctacagaaa aacagtatga aactctcgaa aatcaattag cctttttatg ccaacaaggt
     2161 ttttcactag agaatgcatt atatgcactc agcgctgtgg ggcattttac tttaggttgc
     2221 gtattggaag atcaagagca tcaagtcgct aaagaagaaa gggaaacacc tactactgat
     2281 agtatgccgc cattattacg acaagctatc gaattatttg atcaccaagg tgcagagcca
     2341 gccttcttat tcggccttga attgatcata tgcggattag aaaaacaact taaatgtgaa
     2401 agtgggtccg ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc
     2461 tagtgtagat cactactaga gccggcttat cggtcagttt cacctgattt acgtaaaaac
     2521 ccgcttcggc gggtttttgc ttttggaggg gcagaaagat gaatgactgt ccacgacgct
     2581 atacccaaaa gaaa
//