##gff-version 3
##sequence-region BBa_K539563 1 2594
BBa_K539563	.	CDS	1	543	.	+	0	ID=BBa_K539513;Name=BBa_K539513
BBa_K539563	.	CDS	1	543	.	+	0	ID=annotation2143524;Name=vioC;Parent=BBa_K539513
BBa_K539563	.	engineered_region	552	2594	.	+	0	ID=BBa_K539558;Name=BBa_K539558
BBa_K539563	.	CDS	570	1697	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_K539558
BBa_K539563	.	engineered_tag	1698	1722	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K539563	.	CDS	570	1697	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K539563	.	tag	1659	1697	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K539563	.	engineered_region	570	1697	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K539563	.	ribosome_entry_site	552	563	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K539558
BBa_K539563	.	conserved_region	556	559	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K539563	.	engineered_region	1731	2594	.	+	0	ID=BBa_K332033;Name=BBa_K332033;Parent=BBa_K539558
BBa_K539563	.	CDS	1789	2473	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K332033
BBa_K539563	.	engineered_tag	2449	2473	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K539563	.	tag	2409	2442	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K539563	.	CDS	1792	2408	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K539563	.	mature_transcript_region	1731	1782	.	+	0	ID=BBa_K115002;Name=BBa_K115002;Parent=BBa_K332033
BBa_K539563	.	stem_loop	1731	1748	.	+	0	ID=annotation1966930;Name=stem_loop;Parent=BBa_K115002
BBa_K539563	.	sequence_alteration	1753	1758	.	+	0	ID=annotation1966899;Name=scar adaptation;Parent=BBa_K115002
BBa_K539563	.	ribosome_entry_site	1777	1782	.	+	0	ID=annotation1966897;Name=rbs;Parent=BBa_K115002
BBa_K539563	.	stem_loop	1754	1782	.	+	0	ID=annotation1966931;Name=predicted stem-loop extending to one position before start codon;Parent=BBa_K115002
BBa_K539563	.	terminator	2482	2594	.	+	0	ID=BBa_J61048;Name=BBa_J61048;Parent=BBa_K332033
>BBa_K539563
aatgaaacgtgcgattatcgttggtggcggcctggcgggtggcctgaccgcgatctacctggcgaagcgtggctacgaa
gtgcacgtcgtggagaagcgtggtgatcctctgcgcgatctgagctcttacgtggacgttgttagcagccgtgcgatcg
gcgtgagcatgaccgttcgtggtatcaagagcgttttggctgcgggcattccgcgtgcagagctggatgcgtgtggcga
accgatcgtggcaatggctttctccgtgggtggtcagtatcgcatgcgcgaactgaagccgttggaggatttccgtccg
ctgagcttgaaccgtgcggcgtttcaaaagctgctgaacaaatacgcgaacctggcaggcgttcgttactactttgagc
ataagtgcctggatgttgacctggatggtaagagcgtgttgattcagggcaaagatggtcagccgcagcgtctgcaagg
tgacatgattatcggtgcggatggcgcccacagcgccgtccgtcaggcgatgcagagcggcctgcgtcgtactagagaa
agaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcag
accgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagc
tgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtct
ggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcg
atggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatca
ttaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgt
ctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgca
ttgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcata
aatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaac
catgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgc
gccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgtt
atatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctc
tcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacg
caaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagg
ctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagggacaagc
aatgcttgccttgaatagtaacttttgaatagtgattcaggaggtactagatgtccagattagataaaagtaaagtgat
taacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgta
gagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggc
accatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgc
tttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaat
caattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactt
taggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgcc
attattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcata
tgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataac
actgatagtgctagtgtagatcactactagagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcg
gcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa