LOCUS       BBa_K539631             1943 bp    DNA     linear       22-Dec-2024
DEFINITION  promoter (lacI regulated) with a RBS (B0032)+Alss
ACCESSION   BBa_K539631
VERSION     BBa_K539631.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..221
                     /label=RBS-3\Weak
     RBS             215..218
                     /label=RBS
     misc_feature    209..221
                     /label=BBa_B0032
     RBS             209..221
                     /label=BBa_B0032
     CDS             228..1943
                     /label=AlsS
     CDS             228..1943
                     /label=BBa_K539713
     misc_feature    209..1943
                     /label=BBa_K539627
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg ttgacaaaag
      241 caacaaaaga acaaaaatcc cttgtgaaaa acagaggggc ggagcttgtt gttgattgct
      301 tagtggagca aggtgtcaca catgtatttg gcattccagg tgcaaaaatt gatgcggtat
      361 ttgacgcttt acaagataaa ggacctgaaa ttatcgttgc ccggcacgaa caaaacgcag
      421 cattcatggc ccaagcagtc ggccgtttaa ctggaaaacc gggagtcgtg ttagtcacat
      481 caggaccggg tgcctctaac ttggcaacag gcctgctgac agcgaacact gaaggagacc
      541 ctgtcgttgc gcttgctgga aacgtgatcc gtgcagatcg tttaaaacgg acacatcaat
      601 ctttggataa tgcggcgcta ttccagccga ttacaaaata cagtgtagaa gttcaagatg
      661 taaaaaatat accggaagct gttacaaatg catttaggat agcgtcagca gggcaggctg
      721 gggccgcttt tgtgagcttt ccgcaagatg ttgtgaatga agtcacaaat acgaaaaacg
      781 tgcgtgctgt tgcagcgcca aaactcggtc cagcagcaga tgatgcaatc agtgcggcca
      841 tagcaaaaat ccaaacagca aaacttcctg tcgttttggt cggcatgaaa ggcggaagac
      901 cggaagcaat taaggcggtt cgcaagcttt tgaaaaaggt tcagcttcca tttgttgaaa
      961 catatcaagc tgccggtacc ctttccagag atttagagga tcaatatttt ggccgtatcg
     1021 gtttgttccg caaccagcct ggcgatttac tgctagagca ggcagatgtt gttctgacga
     1081 tcggctatga cccgattgaa tatgatccga aattctggaa tatcaatgga gaccggacaa
     1141 ttatccattt agacgagatt atcgctgaca ttgatcatgc ttaccagcct gatcttgaat
     1201 tgatcggtga cattccgtcc acgatcaatc atatcgaaca cgatgctgtg aaagtggaat
     1261 ttgcagagcg tgagcagaaa atcctttctg atttaaaaca atatatgcat gaaggtgagc
     1321 aggtgccagc agattggaaa tcagacagag cgcaccctct tgaaatcgtt aaagagttgc
     1381 gtaatgcagt cgatgatcat gttacagtaa cttgcgatat cggttcgcac gccatttgga
     1441 tgtcacgtta tttccgcagc tacgagccgt taacattaat gatcagtaac ggtatgcaaa
     1501 cactcggcgt tgcgcttcct tgggcaatcg gcgcttcatt ggtgaaaccg ggagaaaaag
     1561 tggtttctgt ctctggtgac ggcggtttct tattctcagc aatggaatta gagacagcag
     1621 ttcgactaaa agcaccaatt gtacacattg tatggaacga cagcacatat gacatggttg
     1681 cattccagca attgaaaaaa tataaccgta catctgcggt cgatttcgga aatatcgata
     1741 tcgtgaaata tgcggaaagc ttcggagcaa ctggcttgcg cgtagaatca ccagaccagc
     1801 tggcagatgt tctgcgtcaa ggcatgaacg ctgaaggtcc tgtcatcatc gatgtcccgg
     1861 ttgactacag tgataacatt aatttagcaa gtgacaagct tccgaaagaa tttggggaac
     1921 tcatgaaaac gaaagctctc tag
//