BBa_K539652
type: ComponentDefinition
persistent identity: BBa K539652
display id: BBa_K539652
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated) + a RBS (B0032)+Alss with a RBS (B0032)+ilvC
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/Works
group u list: _705_
m user id: 0
owner id: 10239
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: a
star: false
creator: Shu-Han Chang
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K539622
component: BBa_K539631
sequence annotation: BBa_K539631
sequence annotation: BBa_K539622
sequence: https://synbiohub.org/public/igem/BBa_K539652_sequence/1

BBa_K539622
type: Component
persistent identity: component2144158
display id: component2144158
version: 1
top level: BBa_K539652
definition: BBa_K539622
access: public

BBa_K539631
type: Component
persistent identity: component2144152
display id: component2144152
version: 1
top level: BBa_K539652
definition: BBa_K539631
access: public

BBa_K539631
type: SequenceAnnotation
persistent identity: annotation2144152
display id: annotation2144152
version: 1
top level: BBa_K539652
location: https://synbiohub.org/public/igem/BBa_K539652/annotation2144152/range2144152/1
component: BBa_K539631

https://synbiohub.org/public/igem/BBa_K539652/annotation2144152/range2144152/1
type: Range
persistent identity: range2144152
display id: range2144152
version: 1
top level: BBa_K539652
direction: direction/reverse
start: 1
end: 1943
orientation: inline

BBa_K539622
type: SequenceAnnotation
persistent identity: annotation2144158
display id: annotation2144158
version: 1
top level: BBa_K539652
location: https://synbiohub.org/public/igem/BBa_K539652/annotation2144158/range2144158/1
component: BBa_K539622

https://synbiohub.org/public/igem/BBa_K539652/annotation2144158/range2144158/1
type: Range
persistent identity: range2144158
display id: range2144158
version: 1
top level: BBa_K539652
direction: direction/reverse
start: 1952
end: 3446
orientation: inline

BBa_K539622
type: ComponentDefinition
persistent identity: BBa K539622
display id: BBa_K539622
version: 1
was generated by: iGEM to SBOL conversion
description: a RBS (B0032)+ilvC
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: a
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: a
discontinued: false
dominant: false
experience: experience/Works
group u list: _705_
m user id: 0
owner id: 10239
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: a
star: false
creator: Shu-Han Chang
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K539711
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_K539711
sequence: https://synbiohub.org/public/igem/BBa_K539622_sequence/1

BBa_K539711
type: Component
persistent identity: component2144138
display id: component2144138
version: 1
top level: BBa_K539622
definition: BBa_K539711
access: public

BBa_B0032
type: Component
persistent identity: component2144135
display id: component2144135
version: 1
top level: BBa_K539622
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2144135
display id: annotation2144135
version: 1
top level: BBa_K539622
location: https://synbiohub.org/public/igem/BBa_K539622/annotation2144135/range2144135/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K539622/annotation2144135/range2144135/1
type: Range
persistent identity: range2144135
display id: range2144135
version: 1
top level: BBa_K539622
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_K539711
type: SequenceAnnotation
persistent identity: annotation2144138
display id: annotation2144138
version: 1
top level: BBa_K539622
location: https://synbiohub.org/public/igem/BBa_K539622/annotation2144138/range2144138/1
component: BBa_K539711

https://synbiohub.org/public/igem/BBa_K539622/annotation2144138/range2144138/1
type: Range
persistent identity: range2144138
display id: range2144138
version: 1
top level: BBa_K539622
direction: direction/reverse
start: 20
end: 1495
orientation: inline

BBa_K539631
type: ComponentDefinition
persistent identity: BBa K539631
display id: BBa_K539631
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated) with a RBS (B0032)+Alss
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/Works
group u list: _705_
m user id: 0
owner id: 10239
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: a
star: false
creator: Shu-Han Chang
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_K539627
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_K539627
sequence: https://synbiohub.org/public/igem/BBa_K539631_sequence/1

BBa_K539627
type: Component
persistent identity: component2144094
display id: component2144094
version: 1
top level: BBa_K539631
definition: BBa_K539627
access: public

BBa_R0010
type: Component
persistent identity: component2144082
display id: component2144082
version: 1
top level: BBa_K539631
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2144082
display id: annotation2144082
version: 1
top level: BBa_K539631
location: https://synbiohub.org/public/igem/BBa_K539631/annotation2144082/range2144082/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K539631/annotation2144082/range2144082/1
type: Range
persistent identity: range2144082
display id: range2144082
version: 1
top level: BBa_K539631
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_K539627
type: SequenceAnnotation
persistent identity: annotation2144094
display id: annotation2144094
version: 1
top level: BBa_K539631
location: https://synbiohub.org/public/igem/BBa_K539631/annotation2144094/range2144094/1
component: BBa_K539627

https://synbiohub.org/public/igem/BBa_K539631/annotation2144094/range2144094/1
type: Range
persistent identity: range2144094
display id: range2144094
version: 1
top level: BBa_K539631
direction: direction/reverse
start: 209
end: 1943
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_K539627
type: ComponentDefinition
persistent identity: BBa K539627
display id: BBa_K539627
version: 1
was generated by: iGEM to SBOL conversion
description: a RBS (B0032)+Alss
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: a
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: a
discontinued: false
dominant: false
experience: experience/Works
group u list: _705_
m user id: 0
owner id: 10239
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: a
star: false
creator: Shu-Han Chang
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K539713
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_K539713
sequence: https://synbiohub.org/public/igem/BBa_K539627_sequence/1

BBa_K539713
type: Component
persistent identity: component2144007
display id: component2144007
version: 1
top level: BBa_K539627
definition: BBa_K539713
access: public

BBa_B0032
type: Component
persistent identity: component2144004
display id: component2144004
version: 1
top level: BBa_K539627
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2144004
display id: annotation2144004
version: 1
top level: BBa_K539627
location: https://synbiohub.org/public/igem/BBa_K539627/annotation2144004/range2144004/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K539627/annotation2144004/range2144004/1
type: Range
persistent identity: range2144004
display id: range2144004
version: 1
top level: BBa_K539627
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_K539713
type: SequenceAnnotation
persistent identity: annotation2144007
display id: annotation2144007
version: 1
top level: BBa_K539627
location: https://synbiohub.org/public/igem/BBa_K539627/annotation2144007/range2144007/1
component: BBa_K539713

https://synbiohub.org/public/igem/BBa_K539627/annotation2144007/range2144007/1
type: Range
persistent identity: range2144007
display id: range2144007
version: 1
top level: BBa_K539627
direction: direction/reverse
start: 20
end: 1735
orientation: inline

BBa_K539711
type: ComponentDefinition
persistent identity: BBa K539711
display id: BBa_K539711
version: 1
was generated by: iGEM to SBOL conversion
description: ilvC (ketol-acid reductoisomerase NAD(P)-binding)
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: It comes from Escherichia coli str. K-12 substr. MG1655
owned by: james
owned by: myers
mutable description: This is the coding region of ilvC from Escherichia coli str. K-12 substr(MG1655). It encodes ketol-acid reductoisomerase NAD(P)-binding which is part of the isobutanol biosynthesis pathway.
discontinued: false
dominant: false
experience: experience/None
group u list: _705_
m user id: 0
owner id: 10230
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding gene, we have to delete those RE site in kivd. The RE site in the part has been modified by point mutation.
star: false
creator: Yang, Yi-Pei
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ilvC coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K539711_sequence/1

ilvC coding sequence
type: SequenceAnnotation
persistent identity: annotation2136206
display id: annotation2136206
version: 1
top level: BBa_K539711
location: https://synbiohub.org/public/igem/BBa_K539711/annotation2136206/range2136206/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K539711/annotation2136206/range2136206/1
type: Range
persistent identity: range2136206
display id: range2136206
version: 1
top level: BBa_K539711
direction: direction/forward
start: 1
end: 1476
orientation: inline

BBa_K539713
type: ComponentDefinition
persistent identity: BBa K539713
display id: BBa_K539713
version: 1
was generated by: iGEM to SBOL conversion
description: AlsS (alpha-acetolactate synthase protein)
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: It comes from Bacillus subtilis.
owned by: james
owned by: myers
mutable description: This is the coding region of AlsS from Bacillus subtilis. It encodes alpha-acetolactate synthase protein which is part of isobutanol biosynthesis pathway.
discontinued: false
dominant: false
group u list: _705_
m user id: 0
owner id: 10230
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding sequence, those RE site should not exist in coding sequence. There are two Pst1 site, one Xba1 site and one EcoR1 site in original sequence of AlsS. However, those RE site in AlsS has been modified by point mutation.
star: false
creator: Yang, Yi-Pei
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: AlsS
sequence: https://synbiohub.org/public/igem/BBa_K539713_sequence/1

AlsS
type: SequenceAnnotation
persistent identity: annotation2136210
display id: annotation2136210
version: 1
top level: BBa_K539713
location: https://synbiohub.org/public/igem/BBa_K539713/annotation2136210/range2136210/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K539713/annotation2136210/range2136210/1
type: Range
persistent identity: range2136210
display id: range2136210
version: 1
top level: BBa_K539713
direction: direction/forward
start: 1
end: 1716
orientation: inline

https://synbiohub.org/public/igem/BBa_K539652_sequence/1
type: Sequence
persistent identity: BBa K539652 sequence
display id: BBa_K539652_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatgttgacaaaagcaacaaaagaacaaaaatcccttgtgaaaaacagaggggcggagcttgttgttgattgcttagtggagcaaggtgtcacacatgtatttggcattccaggtgcaaaaattgatgcggtatttgacgctttacaagataaaggacctgaaattatcgttgcccggcacgaacaaaacgcagcattcatggcccaagcagtcggccgtttaactggaaaaccgggagtcgtgttagtcacatcaggaccgggtgcctctaacttggcaacaggcctgctgacagcgaacactgaaggagaccctgtcgttgcgcttgctggaaacgtgatccgtgcagatcgtttaaaacggacacatcaatctttggataatgcggcgctattccagccgattacaaaatacagtgtagaagttcaagatgtaaaaaatataccggaagctgttacaaatgcatttaggatagcgtcagcagggcaggctggggccgcttttgtgagctttccgcaagatgttgtgaatgaagtcacaaatacgaaaaacgtgcgtgctgttgcagcgccaaaactcggtccagcagcagatgatgcaatcagtgcggccatagcaaaaatccaaacagcaaaacttcctgtcgttttggtcggcatgaaaggcggaagaccggaagcaattaaggcggttcgcaagcttttgaaaaaggttcagcttccatttgttgaaacatatcaagctgccggtaccctttccagagatttagaggatcaatattttggccgtatcggtttgttccgcaaccagcctggcgatttactgctagagcaggcagatgttgttctgacgatcggctatgacccgattgaatatgatccgaaattctggaatatcaatggagaccggacaattatccatttagacgagattatcgctgacattgatcatgcttaccagcctgatcttgaattgatcggtgacattccgtccacgatcaatcatatcgaacacgatgctgtgaaagtggaatttgcagagcgtgagcagaaaatcctttctgatttaaaacaatatatgcatgaaggtgagcaggtgccagcagattggaaatcagacagagcgcaccctcttgaaatcgttaaagagttgcgtaatgcagtcgatgatcatgttacagtaacttgcgatatcggttcgcacgccatttggatgtcacgttatttccgcagctacgagccgttaacattaatgatcagtaacggtatgcaaacactcggcgttgcgcttccttgggcaatcggcgcttcattggtgaaaccgggagaaaaagtggtttctgtctctggtgacggcggtttcttattctcagcaatggaattagagacagcagttcgactaaaagcaccaattgtacacattgtatggaacgacagcacatatgacatggttgcattccagcaattgaaaaaatataaccgtacatctgcggtcgatttcggaaatatcgatatcgtgaaatatgcggaaagcttcggagcaactggcttgcgcgtagaatcaccagaccagctggcagatgttctgcgtcaaggcatgaacgctgaaggtcctgtcatcatcgatgtcccggttgactacagtgataacattaatttagcaagtgacaagcttccgaaagaatttggggaactcatgaaaacgaaagctctctagtactagagtcacacaggaaagtactagatggctaactacttcaatacactgaatctg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K539622_sequence/1
type: Sequence
persistent identity: BBa K539622 sequence
display id: BBa_K539622_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatggctaactacttcaatacactgaatctgcgccagcagctggcacagctgggcaaatgtcgctttatgggccgcgatgaatttgccgatggcgcgagctaccttcagggtaaaaaagtagtcatcgtcggctgtggcgcacagggtctgaaccagggcctgaacatgcgtgattctggtctcgatatctcctacgctctgcgtaaagaagcgattgccgagaagcgcgcgtcctggcgtaaagcgaccgaaaatggttttaaagtgggtacttacgaagaactgatcccacaggcggatctggtgattaacctgacgccggacaagcagcactctgatgtagtgcgcaccgtacagccactgatgaaagacggcgcggcgctgggctactcgcacggtttcaacatcgtcgaagtgggcgagcagatccgtaaagatatcaccgtagtgatggttgcgccgaaatgcccaggcaccgaagtgcgtgaagagtacaaacgtgggttcggcgtaccgacgctgattgccgttcacccggaaaacgatccgaaaggcgaaggcatggcgattgccaaagcctgggcggctgcaaccggtggtcaccgtgcgggtgtgctggaatcgtccttcgttgcggaagtgaaatctgatctgatgggcgagcaaaccatcctgtgcggtatgttgcaggctggctctctgctgtgcttcgacaagctggtggaagaaggtaccgatccagcatacgcagaaaaactgattcagttcggttgggaaaccatcaccgaagcactgaaacagggcggcatcaccctgatgatggaccgtctctctaacccggcgaaactgcgtgcttatgcgctttctgaacagctgaaagagatcatggcacccctgttccagaaacatatggacgacatcatctccggcgaattttcttccggtatgatggcggactgggccaacgatgataagaaactgctgacctggcgtgaagagaccggcaaaaccgcgtttgaaaccgcgccgcagtatgaaggcaaaatcggcgagcaggagtacttcgataaaggcgtactgatgattgcgatggtgaaagcgggcgttgaactggcgttcgaaaccatggtcgattccggcatcattgaagagtctgcatattatgaatcactgcacgagctgccgctgattgccaacaccatcgcccgtaagcgtctgtacgaaatgaacgtggttatctctgataccgctgagtacggtaactatctgttctcttacgcttgtgtgccgttgctgaaaccgtttatggcagagctgcaaccgggcgacctgggtaaagctattccggaaggcgcggtagataacgggcaactgcgtgatgtgaacgaagcgattcgcagccatgcgattgagcaggtaggtaagaaactgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K539631_sequence/1
type: Sequence
persistent identity: BBa K539631 sequence
display id: BBa_K539631_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatgttgacaaaagcaacaaaagaacaaaaatcccttgtgaaaaacagaggggcggagcttgttgttgattgcttagtggagcaaggtgtcacacatgtatttggcattccaggtgcaaaaattgatgcggtatttgacgctttacaagataaaggacctgaaattatcgttgcccggcacgaacaaaacgcagcattcatggcccaagcagtcggccgtttaactggaaaaccgggagtcgtgttagtcacatcaggaccgggtgcctctaacttggcaacaggcctgctgacagcgaacactgaaggagaccctgtcgttgcgcttgctggaaacgtgatccgtgcagatcgtttaaaacggacacatcaatctttggataatgcggcgctattccagccgattacaaaatacagtgtagaagttcaagatgtaaaaaatataccggaagctgttacaaatgcatttaggatagcgtcagcagggcaggctggggccgcttttgtgagctttccgcaagatgttgtgaatgaagtcacaaatacgaaaaacgtgcgtgctgttgcagcgccaaaactcggtccagcagcagatgatgcaatcagtgcggccatagcaaaaatccaaacagcaaaacttcctgtcgttttggtcggcatgaaaggcggaagaccggaagcaattaaggcggttcgcaagcttttgaaaaaggttcagcttccatttgttgaaacatatcaagctgccggtaccctttccagagatttagaggatcaatattttggccgtatcggtttgttccgcaaccagcctggcgatttactgctagagcaggcagatgttgttctgacgatcggctatgacccgattgaatatgatccgaaattctggaatatcaatggagaccggacaattatccatttagacgagattatcgctgacattgatcatgcttaccagcctgatcttgaattgatcggtgacattccgtccacgatcaatcatatcgaacacgatgctgtgaaagtggaatttgcagagcgtgagcagaaaatcctttctgatttaaaacaatatatgcatgaaggtgagcaggtgccagcagattggaaatcagacagagcgcaccctcttgaaatcgttaaagagttgcgtaatgcagtcgatgatcatgttacagtaacttgcgatatcggttcgcacgccatttggatgtcacgttatttccgcagctacgagccgttaacattaatgatcagtaacggtatgcaaacactcggcgttgcgcttccttgggcaatcggcgcttcattggtgaaaccgggagaaaaagtggtttctgtctctggtgacggcggtttcttattctcagcaatggaattagagacagcagttcgactaaaagcaccaattgtacacattgtatggaacgacagcacatatgacatggttgcattccagcaattgaaaaaatataaccgtacatctgcggtcgatttcggaaatatcgatatcgtgaaatatgcggaaagcttcggagcaactggcttgcgcgtagaatcaccagaccagctggcagatgttctgcgtcaaggcatgaacgctgaaggtcctgtcatcatcgatgtcccggttgactacagtgataacattaatttagcaagtgacaagcttccgaaagaatttggggaactcatgaaaacgaaagctctctag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K539627_sequence/1
type: Sequence
persistent identity: BBa K539627 sequence
display id: BBa_K539627_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgttgacaaaagcaacaaaagaacaaaaatcccttgtgaaaaacagaggggcggagcttgttgttgattgcttagtggagcaaggtgtcacacatgtatttggcattccaggtgcaaaaattgatgcggtatttgacgctttacaagataaaggacctgaaattatcgttgcccggcacgaacaaaacgcagcattcatggcccaagcagtcggccgtttaactggaaaaccgggagtcgtgttagtcacatcaggaccgggtgcctctaacttggcaacaggcctgctgacagcgaacactgaaggagaccctgtcgttgcgcttgctggaaacgtgatccgtgcagatcgtttaaaacggacacatcaatctttggataatgcggcgctattccagccgattacaaaatacagtgtagaagttcaagatgtaaaaaatataccggaagctgttacaaatgcatttaggatagcgtcagcagggcaggctggggccgcttttgtgagctttccgcaagatgttgtgaatgaagtcacaaatacgaaaaacgtgcgtgctgttgcagcgccaaaactcggtccagcagcagatgatgcaatcagtgcggccatagcaaaaatccaaacagcaaaacttcctgtcgttttggtcggcatgaaaggcggaagaccggaagcaattaaggcggttcgcaagcttttgaaaaaggttcagcttccatttgttgaaacatatcaagctgccggtaccctttccagagatttagaggatcaatattttggccgtatcggtttgttccgcaaccagcctggcgatttactgctagagcaggcagatgttgttctgacgatcggctatgacccgattgaatatgatccgaaattctggaatatcaatggagaccggacaattatccatttagacgagattatcgctgacattgatcatgcttaccagcctgatcttgaattgatcggtgacattccgtccacgatcaatcatatcgaacacgatgctgtgaaagtggaatttgcagagcgtgagcagaaaatcctttctgatttaaaacaatatatgcatgaaggtgagcaggtgccagcagattggaaatcagacagagcgcaccctcttgaaatcgttaaagagttgcgtaatgcagtcgatgatcatgttacagtaacttgcgatatcggttcgcacgccatttggatgtcacgttatttccgcagctacgagccgttaacattaatgatcagtaacggtatgcaaacactcggcgttgcgcttccttgggcaatcggcgcttcattggtgaaaccgggagaaaaagtggtttctgtctctggtgacggcggtttcttattctcagcaatggaattagagacagcagttcgactaaaagcaccaattgtacacattgtatggaacgacagcacatatgacatggttgcattccagcaattgaaaaaatataaccgtacatctgcggtcgatttcggaaatatcgatatcgtgaaatatgcggaaagcttcggagcaactggcttgcgcgtagaatcaccagaccagctggcagatgttctgcgtcaaggcatgaacgctgaaggtcctgtcatcatcgatgtcccggttgactacagtgataacattaatttagcaagtgacaagcttccgaaagaatttggggaactcatgaaaacgaaagctctctag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K539711_sequence/1
type: Sequence
persistent identity: BBa K539711 sequence
display id: BBa_K539711_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctaactacttcaatacactgaatctgcgccagcagctggcacagctgggcaaatgtcgctttatgggccgcgatgaatttgccgatggcgcgagctaccttcagggtaaaaaagtagtcatcgtcggctgtggcgcacagggtctgaaccagggcctgaacatgcgtgattctggtctcgatatctcctacgctctgcgtaaagaagcgattgccgagaagcgcgcgtcctggcgtaaagcgaccgaaaatggttttaaagtgggtacttacgaagaactgatcccacaggcggatctggtgattaacctgacgccggacaagcagcactctgatgtagtgcgcaccgtacagccactgatgaaagacggcgcggcgctgggctactcgcacggtttcaacatcgtcgaagtgggcgagcagatccgtaaagatatcaccgtagtgatggttgcgccgaaatgcccaggcaccgaagtgcgtgaagagtacaaacgtgggttcggcgtaccgacgctgattgccgttcacccggaaaacgatccgaaaggcgaaggcatggcgattgccaaagcctgggcggctgcaaccggtggtcaccgtgcgggtgtgctggaatcgtccttcgttgcggaagtgaaatctgatctgatgggcgagcaaaccatcctgtgcggtatgttgcaggctggctctctgctgtgcttcgacaagctggtggaagaaggtaccgatccagcatacgcagaaaaactgattcagttcggttgggaaaccatcaccgaagcactgaaacagggcggcatcaccctgatgatggaccgtctctctaacccggcgaaactgcgtgcttatgcgctttctgaacagctgaaagagatcatggcacccctgttccagaaacatatggacgacatcatctccggcgaattttcttccggtatgatggcggactgggccaacgatgataagaaactgctgacctggcgtgaagagaccggcaaaaccgcgtttgaaaccgcgccgcagtatgaaggcaaaatcggcgagcaggagtacttcgataaaggcgtactgatgattgcgatggtgaaagcgggcgttgaactggcgttcgaaaccatggtcgattccggcatcattgaagagtctgcatattatgaatcactgcacgagctgccgctgattgccaacaccatcgcccgtaagcgtctgtacgaaatgaacgtggttatctctgataccgctgagtacggtaactatctgttctcttacgcttgtgtgccgttgctgaaaccgtttatggcagagctgcaaccgggcgacctgggtaaagctattccggaaggcgcggtagataacgggcaactgcgtgatgtgaacgaagcgattcgcagccatgcgattgagcaggtaggtaagaaactgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K539713_sequence/1
type: Sequence
persistent identity: BBa K539713 sequence
display id: BBa_K539713_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttgacaaaagcaacaaaagaacaaaaatcccttgtgaaaaacagaggggcggagcttgttgttgattgcttagtggagcaaggtgtcacacatgtatttggcattccaggtgcaaaaattgatgcggtatttgacgctttacaagataaaggacctgaaattatcgttgcccggcacgaacaaaacgcagcattcatggcccaagcagtcggccgtttaactggaaaaccgggagtcgtgttagtcacatcaggaccgggtgcctctaacttggcaacaggcctgctgacagcgaacactgaaggagaccctgtcgttgcgcttgctggaaacgtgatccgtgcagatcgtttaaaacggacacatcaatctttggataatgcggcgctattccagccgattacaaaatacagtgtagaagttcaagatgtaaaaaatataccggaagctgttacaaatgcatttaggatagcgtcagcagggcaggctggggccgcttttgtgagctttccgcaagatgttgtgaatgaagtcacaaatacgaaaaacgtgcgtgctgttgcagcgccaaaactcggtccagcagcagatgatgcaatcagtgcggccatagcaaaaatccaaacagcaaaacttcctgtcgttttggtcggcatgaaaggcggaagaccggaagcaattaaggcggttcgcaagcttttgaaaaaggttcagcttccatttgttgaaacatatcaagctgccggtaccctttccagagatttagaggatcaatattttggccgtatcggtttgttccgcaaccagcctggcgatttactgctagagcaggcagatgttgttctgacgatcggctatgacccgattgaatatgatccgaaattctggaatatcaatggagaccggacaattatccatttagacgagattatcgctgacattgatcatgcttaccagcctgatcttgaattgatcggtgacattccgtccacgatcaatcatatcgaacacgatgctgtgaaagtggaatttgcagagcgtgagcagaaaatcctttctgatttaaaacaatatatgcatgaaggtgagcaggtgccagcagattggaaatcagacagagcgcaccctcttgaaatcgttaaagagttgcgtaatgcagtcgatgatcatgttacagtaacttgcgatatcggttcgcacgccatttggatgtcacgttatttccgcagctacgagccgttaacattaatgatcagtaacggtatgcaaacactcggcgttgcgcttccttgggcaatcggcgcttcattggtgaaaccgggagaaaaagtggtttctgtctctggtgacggcggtttcttattctcagcaatggaattagagacagcagttcgactaaaagcaccaattgtacacattgtatggaacgacagcacatatgacatggttgcattccagcaattgaaaaaatataaccgtacatctgcggtcgatttcggaaatatcgatatcgtgaaatatgcggaaagcttcggagcaactggcttgcgcgtagaatcaccagaccagctggcagatgttctgcgtcaaggcatgaacgctgaaggtcctgtcatcatcgatgtcccggttgactacagtgataacattaatttagcaagtgacaagcttccgaaagaatttggggaactcatgaaaacgaaagctctctag
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z