LOCUS       BBa_K539653             2744 bp    DNA     linear       22-Nov-2024
DEFINITION  a RBS (B0030)+ilvD with RNA thermometer+tetR+terminator
ACCESSION   BBa_K539653
VERSION     BBa_K539653.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     CDS             22..1872
                     /label=ilvD
     CDS             22..1872
                     /label=BBa_K539712
     misc_feature    1..1872
                     /label=BBa_K539624
     RBS             1927..1932
                     /label=rbs
     misc_feature    1903..1908
                     /label=scar adaptation
     stem_loop       1881..1898
                     /label=stem_loop
     stem_loop       1904..1932
                     /label=predicted stem-loop extending to one position 
                     before start codon
     misc_feature    1881..1932
                     /label=BBa_K115002
     CDS             1942..2558
                     /label=tetR
     misc_feature    2559..2592
                     /label=SsrA
     misc_feature    2599..2623
                     /label=Help:Barcodes
     CDS             1939..2623
                     /label=BBa_C0040
     terminator      2632..2744
                     /label=BBa_J61048
     misc_feature    1881..2744
                     /label=BBa_K332033
ORIGIN
        1 attaaagagg agaaatacta gatgcctaag taccgttccg ccaccaccac tcatggtcgt
       61 aatatggcgg gtgctcgtgc gctgtggcgc gccaccggaa tgaccgacgc cgatttcggt
      121 aagccgatta tcgcggttgt gaactcgttc acccaatttg taccgggtca cgtccatctg
      181 cgcgatctcg gtaaactggt cgccgaacaa attgaagcgg ctggcggcgt tgccaaagag
      241 ttcaacacca ttgcggtgga tgatgggatt gccatgggcc acggggggat gctttattca
      301 ctgccatctc gcgaactgat cgctgattcc gttgagtata tggtcaacgc ccactgcgcc
      361 gacgccatgg tctgcatctc taactgcgac aaaatcaccc cggggatgct gatggcttcc
      421 ctgcgcctga atattccggt gatctttgtt tccggcggcc cgatggaggc cgggaaaacc
      481 aaactttccg atcagatcat caagctcgat ctggtcgatg cgatgatcca gggcgcagac
      541 ccgaaagtat ctgactccca gagcgatcag gttgaacgtt ccgcgtgtcc gacctgcggt
      601 tcctgctccg ggatgtttac cgctaactca atgaactgcc tgaccgaagc gctgggcctg
      661 tcgcagccgg gcaacggctc gctgctggca acccacgccg accgtaagca gctgttcctt
      721 aatgctggta aacgcattgt tgaattgacc aaacgttatt acgagcaaaa cgacgaaagt
      781 gcactgccgc gtaatatcgc cagtaaggcg gcgtttgaaa acgccatgac gctggatatc
      841 gcgatgggtg gatcgactaa caccgtactt cacctgctgg cggcggcgca ggaagcggaa
      901 atcgacttca ccatgagtga tatcgataag ctttcccgca aggttccaca gctgtgtaaa
      961 gttgcgccga gcacccagaa ataccatatg gaagatgttc accgtgctgg tggtgttatc
     1021 ggtattctcg gcgaactgga tcgcgcgggg ttactgaacc gtgatgtgaa aaacgtactt
     1081 ggcctgacgt tgccgcaaac gctggaacaa tacgacgtta tgctgaccca ggatgacgcg
     1141 gtaaaaaata tgttccgcgc aggtccagca ggcattcgta ccacacaggc attctcgcaa
     1201 gattgccgtt gggatacgct ggacgacgat cgcgccaatg gctgtatccg ctcgctggaa
     1261 cacgcctaca gcaaagacgg cggcctggcg gtgctctacg gtaactttgc ggaaaacggc
     1321 tgcatcgtga aaacggcagg cgtcgatgac agcatcctca aattcaccgg cccggcgaaa
     1381 gtgtacgaaa gccaggacga tgcggtagaa gcgattctcg gcggtaaagt tgtcgccgga
     1441 gatgtggtag taattcgcta tgaaggcccg aaaggcggtc cggggatgca ggaaatgctc
     1501 tacccaacca gcttcctgaa atcaatgggt ctcggcaaag cctgtgcgct gatcaccgac
     1561 ggtcgtttct ctggtggcac ctctggtctt tccatcggcc acgtctcacc ggaagcggca
     1621 agcggcggca gcattggcct gattgaagat ggtgacctga tcgctatcga catcccgaac
     1681 cgtggcattc agttacaggt aagcgatgcc gaactggcgg cgcgtcgtga agcgcaggac
     1741 gctcgaggtg acaaagcctg gacgccgaaa aatcgtgaac gtcaggtctc ctttgccctg
     1801 cgtgcttatg ccagcctggc aaccagcgcc gacaaaggcg cggtgcgcga taaatcgaaa
     1861 ctggggggtt aatactagag ggacaagcaa tgcttgcctt gaatagtaac ttttgaatag
     1921 tgattcagga ggtactagat gtccagatta gataaaagta aagtgattaa cagcgcatta
     1981 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
     2041 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
     2101 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
     2161 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
     2221 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
     2281 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
     2341 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
     2401 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
     2461 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
     2521 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac
     2581 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gccggcttat
     2641 cggtcagttt cacctgattt acgtaaaaac ccgcttcggc gggtttttgc ttttggaggg
     2701 gcagaaagat gaatgactgt ccacgacgct atacccaaaa gaaa
//