##gff-version 3
##sequence-region BBa_K539653 1 2744
BBa_K539653	.	engineered_region	1	1872	.	+	0	ID=BBa_K539624;Name=BBa_K539624
BBa_K539653	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K539624
BBa_K539653	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K539653	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K539653	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K539653	.	CDS	22	1872	.	+	0	ID=BBa_K539712;Name=BBa_K539712;Parent=BBa_K539624
BBa_K539653	.	CDS	22	1872	.	+	0	ID=annotation2136209;Name=ilvD;Parent=BBa_K539712
BBa_K539653	.	engineered_region	1881	2744	.	+	0	ID=BBa_K332033;Name=BBa_K332033
BBa_K539653	.	CDS	1939	2623	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K332033
BBa_K539653	.	tag	2559	2592	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K539653	.	engineered_tag	2599	2623	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K539653	.	CDS	1942	2558	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K539653	.	mature_transcript_region	1881	1932	.	+	0	ID=BBa_K115002;Name=BBa_K115002;Parent=BBa_K332033
BBa_K539653	.	sequence_alteration	1903	1908	.	+	0	ID=annotation1966899;Name=scar adaptation;Parent=BBa_K115002
BBa_K539653	.	ribosome_entry_site	1927	1932	.	+	0	ID=annotation1966897;Name=rbs;Parent=BBa_K115002
BBa_K539653	.	stem_loop	1881	1898	.	+	0	ID=annotation1966930;Name=stem_loop;Parent=BBa_K115002
BBa_K539653	.	stem_loop	1904	1932	.	+	0	ID=annotation1966931;Name=predicted stem-loop extending to one position before start codon;Parent=BBa_K115002
BBa_K539653	.	terminator	2632	2744	.	+	0	ID=BBa_J61048;Name=BBa_J61048;Parent=BBa_K332033
>BBa_K539653
attaaagaggagaaatactagatgcctaagtaccgttccgccaccaccactcatggtcgtaatatggcgggtgctcgtg
cgctgtggcgcgccaccggaatgaccgacgccgatttcggtaagccgattatcgcggttgtgaactcgttcacccaatt
tgtaccgggtcacgtccatctgcgcgatctcggtaaactggtcgccgaacaaattgaagcggctggcggcgttgccaaa
gagttcaacaccattgcggtggatgatgggattgccatgggccacggggggatgctttattcactgccatctcgcgaac
tgatcgctgattccgttgagtatatggtcaacgcccactgcgccgacgccatggtctgcatctctaactgcgacaaaat
caccccggggatgctgatggcttccctgcgcctgaatattccggtgatctttgtttccggcggcccgatggaggccggg
aaaaccaaactttccgatcagatcatcaagctcgatctggtcgatgcgatgatccagggcgcagacccgaaagtatctg
actcccagagcgatcaggttgaacgttccgcgtgtccgacctgcggttcctgctccgggatgtttaccgctaactcaat
gaactgcctgaccgaagcgctgggcctgtcgcagccgggcaacggctcgctgctggcaacccacgccgaccgtaagcag
ctgttccttaatgctggtaaacgcattgttgaattgaccaaacgttattacgagcaaaacgacgaaagtgcactgccgc
gtaatatcgccagtaaggcggcgtttgaaaacgccatgacgctggatatcgcgatgggtggatcgactaacaccgtact
tcacctgctggcggcggcgcaggaagcggaaatcgacttcaccatgagtgatatcgataagctttcccgcaaggttcca
cagctgtgtaaagttgcgccgagcacccagaaataccatatggaagatgttcaccgtgctggtggtgttatcggtattc
tcggcgaactggatcgcgcggggttactgaaccgtgatgtgaaaaacgtacttggcctgacgttgccgcaaacgctgga
acaatacgacgttatgctgacccaggatgacgcggtaaaaaatatgttccgcgcaggtccagcaggcattcgtaccaca
caggcattctcgcaagattgccgttgggatacgctggacgacgatcgcgccaatggctgtatccgctcgctggaacacg
cctacagcaaagacggcggcctggcggtgctctacggtaactttgcggaaaacggctgcatcgtgaaaacggcaggcgt
cgatgacagcatcctcaaattcaccggcccggcgaaagtgtacgaaagccaggacgatgcggtagaagcgattctcggc
ggtaaagttgtcgccggagatgtggtagtaattcgctatgaaggcccgaaaggcggtccggggatgcaggaaatgctct
acccaaccagcttcctgaaatcaatgggtctcggcaaagcctgtgcgctgatcaccgacggtcgtttctctggtggcac
ctctggtctttccatcggccacgtctcaccggaagcggcaagcggcggcagcattggcctgattgaagatggtgacctg
atcgctatcgacatcccgaaccgtggcattcagttacaggtaagcgatgccgaactggcggcgcgtcgtgaagcgcagg
acgctcgaggtgacaaagcctggacgccgaaaaatcgtgaacgtcaggtctcctttgccctgcgtgcttatgccagcct
ggcaaccagcgccgacaaaggcgcggtgcgcgataaatcgaaactggggggttaatactagagggacaagcaatgcttg
ccttgaatagtaacttttgaatagtgattcaggaggtactagatgtccagattagataaaagtaaagtgattaacagcg
cattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcc
tacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatact
cacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaa
gtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagc
ctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgc
gtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattac
gacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggatt
agaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatag
tgctagtgtagatcactactagagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggttt
ttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa