BBa_K539653
type: ComponentDefinition
persistent identity: BBa K539653
display id: BBa_K539653
version: 1
was generated by: iGEM to SBOL conversion
description: a RBS (B0030)+ilvD with RNA thermometer+tetR+terminator
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/Works
group u list: _705_
m user id: 0
owner id: 10239
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: a
star: false
creator: Shu-Han Chang
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K332033
component: BBa_K539624
sequence annotation: BBa_K539624
sequence annotation: BBa_K332033
sequence: https://synbiohub.org/public/igem/BBa_K539653_sequence/1

BBa_K332033
type: Component
persistent identity: component2258850
display id: component2258850
version: 1
top level: BBa_K539653
definition: BBa_K332033
access: public

BBa_K539624
type: Component
persistent identity: component2258839
display id: component2258839
version: 1
top level: BBa_K539653
definition: BBa_K539624
access: public

BBa_K539624
type: SequenceAnnotation
persistent identity: annotation2258839
display id: annotation2258839
version: 1
top level: BBa_K539653
location: https://synbiohub.org/public/igem/BBa_K539653/annotation2258839/range2258839/1
component: BBa_K539624

https://synbiohub.org/public/igem/BBa_K539653/annotation2258839/range2258839/1
type: Range
persistent identity: range2258839
display id: range2258839
version: 1
top level: BBa_K539653
direction: direction/reverse
start: 1
end: 1872
orientation: inline

BBa_K332033
type: SequenceAnnotation
persistent identity: annotation2258850
display id: annotation2258850
version: 1
top level: BBa_K539653
location: https://synbiohub.org/public/igem/BBa_K539653/annotation2258850/range2258850/1
component: BBa_K332033

https://synbiohub.org/public/igem/BBa_K539653/annotation2258850/range2258850/1
type: Range
persistent identity: range2258850
display id: range2258850
version: 1
top level: BBa_K539653
direction: direction/reverse
start: 1881
end: 2744
orientation: inline

BBa_K332033
type: ComponentDefinition
persistent identity: BBa K332033
display id: BBa_K332033
version: 1
was generated by: iGEM to SBOL conversion
description: 37℃ induced RBS + tetR + double terminator
created: 2010-10-23T11:00:00Z
modified: 2015-05-08T01:12:05Z
mutable provenance: the 37℃ induced RBS cames from iGEM08_TUDelft
owned by: james
owned by: myers
mutable description: It is a transcriptional unit including 37℃ induced RBS + tetR + double terminator. It can be added various promoter to adapt your conditions.
discontinued: false
dominant: false
experience: experience/Works
group u list: _452_
m user id: 0
owner id: 7059
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: To regulate following genes by temperature. To maintain cells at 37?? C ↑ : the tetR protein be generated and repress Ptet's circuit. To release cells at 37?? C ↓ : the tetR protein not be generated and do not repress Ptet's circuit.
star: false
creator: KUO, CHIA-CHUN
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
component: BBa_J61048
component: BBa_C0040
component: BBa_K115002
sequence annotation: BBa_K115002
sequence annotation: BBa_C0040
sequence annotation: BBa_J61048
sequence: https://synbiohub.org/public/igem/BBa_K332033_sequence/1

BBa_J61048
type: Component
persistent identity: component2247616
display id: component2247616
version: 1
top level: BBa_K332033
definition: BBa_J61048
access: public

BBa_C0040
type: Component
persistent identity: component2247615
display id: component2247615
version: 1
top level: BBa_K332033
definition: tetR
access: public

BBa_K115002
type: Component
persistent identity: component2247611
display id: component2247611
version: 1
top level: BBa_K332033
definition: BBa_K115002
access: public

BBa_K115002
type: SequenceAnnotation
persistent identity: annotation2247611
display id: annotation2247611
version: 1
top level: BBa_K332033
location: https://synbiohub.org/public/igem/BBa_K332033/annotation2247611/range2247611/1
component: BBa_K115002

https://synbiohub.org/public/igem/BBa_K332033/annotation2247611/range2247611/1
type: Range
persistent identity: range2247611
display id: range2247611
version: 1
top level: BBa_K332033
direction: direction/reverse
start: 1
end: 52
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2247615
display id: annotation2247615
version: 1
top level: BBa_K332033
location: https://synbiohub.org/public/igem/BBa_K332033/annotation2247615/range2247615/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K332033/annotation2247615/range2247615/1
type: Range
persistent identity: range2247615
display id: range2247615
version: 1
top level: BBa_K332033
direction: direction/reverse
start: 59
end: 743
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2247616
display id: annotation2247616
version: 1
top level: BBa_K332033
location: https://synbiohub.org/public/igem/BBa_K332033/annotation2247616/range2247616/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K332033/annotation2247616/range2247616/1
type: Range
persistent identity: range2247616
display id: range2247616
version: 1
top level: BBa_K332033
direction: direction/reverse
start: 752
end: 864
orientation: inline

BBa_K539624
type: ComponentDefinition
persistent identity: BBa K539624
display id: BBa_K539624
version: 1
was generated by: iGEM to SBOL conversion
description: a RBS (B0030)+ilvD
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/Works
group u list: _705_
m user id: 0
owner id: 10239
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: a
star: false
creator: Shu-Han Chang
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K539712
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_K539712
sequence: https://synbiohub.org/public/igem/BBa_K539624_sequence/1

BBa_K539712
type: Component
persistent identity: component2143920
display id: component2143920
version: 1
top level: BBa_K539624
definition: BBa_K539712
access: public

BBa_B0030
type: Component
persistent identity: component2143917
display id: component2143917
version: 1
top level: BBa_K539624
definition: BBa_B0030
access: public

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2143917
display id: annotation2143917
version: 1
top level: BBa_K539624
location: https://synbiohub.org/public/igem/BBa_K539624/annotation2143917/range2143917/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K539624/annotation2143917/range2143917/1
type: Range
persistent identity: range2143917
display id: range2143917
version: 1
top level: BBa_K539624
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_K539712
type: SequenceAnnotation
persistent identity: annotation2143920
display id: annotation2143920
version: 1
top level: BBa_K539624
location: https://synbiohub.org/public/igem/BBa_K539624/annotation2143920/range2143920/1
component: BBa_K539712

https://synbiohub.org/public/igem/BBa_K539624/annotation2143920/range2143920/1
type: Range
persistent identity: range2143920
display id: range2143920
version: 1
top level: BBa_K539624
direction: direction/reverse
start: 22
end: 1872
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

BBa_K115002
type: ComponentDefinition
persistent identity: BBa K115002
display id: BBa_K115002
version: 1
was generated by: iGEM to SBOL conversion
description: RNA thermometer (FourU)
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:09:27Z
mutable provenance: Salmonella Enterica Tyhpy (CP000886.1)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Thermo sensitive small RNA (ROSE structure) which can be used as a RNA regulator.
discontinued: false
dominant: false
experience: experience/None
group u list: _223_
m user id: 0
owner id: 3006
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Part of the sequence is altered because of the scar...
star: true
creator: Bastiaan van den Berg
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence annotation: stem_loop
sequence annotation: scar adaptation
sequence annotation: predicted stem-loop extending to one position before start codon
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K115002_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation1966930
display id: annotation1966930
version: 1
top level: BBa_K115002
location: https://synbiohub.org/public/igem/BBa_K115002/annotation1966930/range1966930/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K115002/annotation1966930/range1966930/1
type: Range
persistent identity: range1966930
display id: range1966930
version: 1
top level: BBa_K115002
direction: direction/forward
start: 1
end: 18
orientation: inline

scar adaptation
type: SequenceAnnotation
persistent identity: annotation1966899
display id: annotation1966899
version: 1
top level: BBa_K115002
location: https://synbiohub.org/public/igem/BBa_K115002/annotation1966899/range1966899/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K115002/annotation1966899/range1966899/1
type: Range
persistent identity: range1966899
display id: range1966899
version: 1
top level: BBa_K115002
direction: direction/forward
start: 23
end: 28
orientation: inline

predicted stem-loop extending to one position before start codon
type: SequenceAnnotation
persistent identity: annotation1966931
display id: annotation1966931
version: 1
top level: BBa_K115002
location: https://synbiohub.org/public/igem/BBa_K115002/annotation1966931/range1966931/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K115002/annotation1966931/range1966931/1
type: Range
persistent identity: range1966931
display id: range1966931
version: 1
top level: BBa_K115002
direction: direction/forward
start: 24
end: 52
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1966897
display id: annotation1966897
version: 1
top level: BBa_K115002
location: https://synbiohub.org/public/igem/BBa_K115002/annotation1966897/range1966897/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K115002/annotation1966897/range1966897/1
type: Range
persistent identity: range1966897
display id: range1966897
version: 1
top level: BBa_K115002
direction: direction/forward
start: 47
end: 52
orientation: inline

BBa_K539712
type: ComponentDefinition
persistent identity: BBa K539712
display id: BBa_K539712
version: 1
was generated by: iGEM to SBOL conversion
description: ilvD (dihydroxyacid dehydratase)
created: 2011-09-19T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: It comes from Escherichia coli str. K-12 substr. MG1655
owned by: james
owned by: myers
mutable description: This is the coding sequence of ilvD form Escherichia coli str. K-12 substr. MG1655. It encodes dihydroxyacid dehydratase which is part of isobutanol biosynthesis pathway.
discontinued: false
dominant: false
group u list: _705_
m user id: 0
owner id: 10230
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding sequence, those RE site should not exist in coding sequence. There is one Pst1 in original sequence of ilvD. However, the RE site in ilvD has been modified by point mutation.
star: false
creator: Yang, Yi-Pei
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: ilvD
sequence: https://synbiohub.org/public/igem/BBa_K539712_sequence/1

ilvD
type: SequenceAnnotation
persistent identity: annotation2136209
display id: annotation2136209
version: 1
top level: BBa_K539712
location: https://synbiohub.org/public/igem/BBa_K539712/annotation2136209/range2136209/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K539712/annotation2136209/range2136209/1
type: Range
persistent identity: range2136209
display id: range2136209
version: 1
top level: BBa_K539712
direction: direction/forward
start: 1
end: 1851
orientation: inline

BBa_J61048
type: ComponentDefinition
persistent identity: BBa J61048
display id: BBa_J61048
version: 1
was generated by: iGEM to SBOL conversion
description: [rnpB-T1] Terminator
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: bob
owned by: james
owned by: myers
mutable description: bob
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: bob
star: true
creator: John Anderson
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence: https://synbiohub.org/public/igem/BBa_J61048_sequence/1

https://synbiohub.org/public/igem/BBa_K539653_sequence/1
type: Sequence
persistent identity: BBa K539653 sequence
display id: BBa_K539653_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatgcctaagtaccgttccgccaccaccactcatggtcgtaatatggcgggtgctcgtgcgctgtggcgcgccaccggaatgaccgacgccgatttcggtaagccgattatcgcggttgtgaactcgttcacccaatttgtaccgggtcacgtccatctgcgcgatctcggtaaactggtcgccgaacaaattgaagcggctggcggcgttgccaaagagttcaacaccattgcggtggatgatgggattgccatgggccacggggggatgctttattcactgccatctcgcgaactgatcgctgattccgttgagtatatggtcaacgcccactgcgccgacgccatggtctgcatctctaactgcgacaaaatcaccccggggatgctgatggcttccctgcgcctgaatattccggtgatctttgtttccggcggcccgatggaggccgggaaaaccaaactttccgatcagatcatcaagctcgatctggtcgatgcgatgatccagggcgcagacccgaaagtatctgactcccagagcgatcaggttgaacgttccgcgtgtccgacctgcggttcctgctccgggatgtttaccgctaactcaatgaactgcctgaccgaagcgctgggcctgtcgcagccgggcaacggctcgctgctggcaacccacgccgaccgtaagcagctgttccttaatgctggtaaacgcattgttgaattgaccaaacgttattacgagcaaaacgacgaaagtgcactgccgcgtaatatcgccagtaaggcggcgtttgaaaacgccatgacgctggatatcgcgatgggtggatcgactaacaccgtacttcacctgctggcggcggcgcaggaagcggaaatcgacttcaccatgagtgatatcgataagctttcccgcaaggttccacagctgtgtaaagttgcgccgagcacccagaaataccatatggaagatgttcaccgtgctggtggtgttatcggtattctcggcgaactggatcgcgcggggttactgaaccgtgatgtgaaaaacgtacttggcctgacgttgccgcaaacgctggaacaatacgacgttatgctgacccaggatgacgcggtaaaaaatatgttccgcgcaggtccagcaggcattcgtaccacacaggcattctcgcaagattgccgttgggatacgctggacgacgatcgcgccaatggctgtatccgctcgctggaacacgcctacagcaaagacggcggcctggcggtgctctacggtaactttgcggaaaacggctgcatcgtgaaaacggcaggcgtcgatgacagcatcctcaaattcaccggcccggcgaaagtgtacgaaagccaggacgatgcggtagaagcgattctcggcggtaaagttgtcgccggagatgtggtagtaattcgctatgaaggcccgaaaggcggtccggggatgcaggaaatgctctacccaaccagcttcctgaaatcaatgggtctcggcaaagcctgtgcgctgatcaccgacggtcgtttctctggtggcacctctggtctttccatcggccacgtctcaccggaagcggcaagcggcggcagcattggcctgattgaagatggtgacctgatcgctatcgacatcccgaaccgtggcattcagttacaggtaagcgatgccgaactggcggcgcgtcgtgaagcgcaggacgctcgaggtgacaaagcctggacgccgaaaaatcgtgaacgtcaggtctcctttgccctgcgtgcttatgccagcctggcaaccagcgccgacaaaggcgcggtgcgcgataaatcgaaactggggggttaatactagagggacaagcaatgcttgccttgaatagtaacttttgaatagtgattcaggaggtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K332033_sequence/1
type: Sequence
persistent identity: BBa K332033 sequence
display id: BBa_K332033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggacaagcaatgcttgccttgaatagtaacttttgaatagtgattcaggaggtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K539624_sequence/1
type: Sequence
persistent identity: BBa K539624 sequence
display id: BBa_K539624_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatgcctaagtaccgttccgccaccaccactcatggtcgtaatatggcgggtgctcgtgcgctgtggcgcgccaccggaatgaccgacgccgatttcggtaagccgattatcgcggttgtgaactcgttcacccaatttgtaccgggtcacgtccatctgcgcgatctcggtaaactggtcgccgaacaaattgaagcggctggcggcgttgccaaagagttcaacaccattgcggtggatgatgggattgccatgggccacggggggatgctttattcactgccatctcgcgaactgatcgctgattccgttgagtatatggtcaacgcccactgcgccgacgccatggtctgcatctctaactgcgacaaaatcaccccggggatgctgatggcttccctgcgcctgaatattccggtgatctttgtttccggcggcccgatggaggccgggaaaaccaaactttccgatcagatcatcaagctcgatctggtcgatgcgatgatccagggcgcagacccgaaagtatctgactcccagagcgatcaggttgaacgttccgcgtgtccgacctgcggttcctgctccgggatgtttaccgctaactcaatgaactgcctgaccgaagcgctgggcctgtcgcagccgggcaacggctcgctgctggcaacccacgccgaccgtaagcagctgttccttaatgctggtaaacgcattgttgaattgaccaaacgttattacgagcaaaacgacgaaagtgcactgccgcgtaatatcgccagtaaggcggcgtttgaaaacgccatgacgctggatatcgcgatgggtggatcgactaacaccgtacttcacctgctggcggcggcgcaggaagcggaaatcgacttcaccatgagtgatatcgataagctttcccgcaaggttccacagctgtgtaaagttgcgccgagcacccagaaataccatatggaagatgttcaccgtgctggtggtgttatcggtattctcggcgaactggatcgcgcggggttactgaaccgtgatgtgaaaaacgtacttggcctgacgttgccgcaaacgctggaacaatacgacgttatgctgacccaggatgacgcggtaaaaaatatgttccgcgcaggtccagcaggcattcgtaccacacaggcattctcgcaagattgccgttgggatacgctggacgacgatcgcgccaatggctgtatccgctcgctggaacacgcctacagcaaagacggcggcctggcggtgctctacggtaactttgcggaaaacggctgcatcgtgaaaacggcaggcgtcgatgacagcatcctcaaattcaccggcccggcgaaagtgtacgaaagccaggacgatgcggtagaagcgattctcggcggtaaagttgtcgccggagatgtggtagtaattcgctatgaaggcccgaaaggcggtccggggatgcaggaaatgctctacccaaccagcttcctgaaatcaatgggtctcggcaaagcctgtgcgctgatcaccgacggtcgtttctctggtggcacctctggtctttccatcggccacgtctcaccggaagcggcaagcggcggcagcattggcctgattgaagatggtgacctgatcgctatcgacatcccgaaccgtggcattcagttacaggtaagcgatgccgaactggcggcgcgtcgtgaagcgcaggacgctcgaggtgacaaagcctggacgccgaaaaatcgtgaacgtcaggtctcctttgccctgcgtgcttatgccagcctggcaaccagcgccgacaaaggcgcggtgcgcgataaatcgaaactggggggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K115002_sequence/1
type: Sequence
persistent identity: BBa K115002 sequence
display id: BBa_K115002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggacaagcaatgcttgccttgaatagtaacttttgaatagtgattcaggagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K539712_sequence/1
type: Sequence
persistent identity: BBa K539712 sequence
display id: BBa_K539712_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcctaagtaccgttccgccaccaccactcatggtcgtaatatggcgggtgctcgtgcgctgtggcgcgccaccggaatgaccgacgccgatttcggtaagccgattatcgcggttgtgaactcgttcacccaatttgtaccgggtcacgtccatctgcgcgatctcggtaaactggtcgccgaacaaattgaagcggctggcggcgttgccaaagagttcaacaccattgcggtggatgatgggattgccatgggccacggggggatgctttattcactgccatctcgcgaactgatcgctgattccgttgagtatatggtcaacgcccactgcgccgacgccatggtctgcatctctaactgcgacaaaatcaccccggggatgctgatggcttccctgcgcctgaatattccggtgatctttgtttccggcggcccgatggaggccgggaaaaccaaactttccgatcagatcatcaagctcgatctggtcgatgcgatgatccagggcgcagacccgaaagtatctgactcccagagcgatcaggttgaacgttccgcgtgtccgacctgcggttcctgctccgggatgtttaccgctaactcaatgaactgcctgaccgaagcgctgggcctgtcgcagccgggcaacggctcgctgctggcaacccacgccgaccgtaagcagctgttccttaatgctggtaaacgcattgttgaattgaccaaacgttattacgagcaaaacgacgaaagtgcactgccgcgtaatatcgccagtaaggcggcgtttgaaaacgccatgacgctggatatcgcgatgggtggatcgactaacaccgtacttcacctgctggcggcggcgcaggaagcggaaatcgacttcaccatgagtgatatcgataagctttcccgcaaggttccacagctgtgtaaagttgcgccgagcacccagaaataccatatggaagatgttcaccgtgctggtggtgttatcggtattctcggcgaactggatcgcgcggggttactgaaccgtgatgtgaaaaacgtacttggcctgacgttgccgcaaacgctggaacaatacgacgttatgctgacccaggatgacgcggtaaaaaatatgttccgcgcaggtccagcaggcattcgtaccacacaggcattctcgcaagattgccgttgggatacgctggacgacgatcgcgccaatggctgtatccgctcgctggaacacgcctacagcaaagacggcggcctggcggtgctctacggtaactttgcggaaaacggctgcatcgtgaaaacggcaggcgtcgatgacagcatcctcaaattcaccggcccggcgaaagtgtacgaaagccaggacgatgcggtagaagcgattctcggcggtaaagttgtcgccggagatgtggtagtaattcgctatgaaggcccgaaaggcggtccggggatgcaggaaatgctctacccaaccagcttcctgaaatcaatgggtctcggcaaagcctgtgcgctgatcaccgacggtcgtttctctggtggcacctctggtctttccatcggccacgtctcaccggaagcggcaagcggcggcagcattggcctgattgaagatggtgacctgatcgctatcgacatcccgaaccgtggcattcagttacaggtaagcgatgccgaactggcggcgcgtcgtgaagcgcaggacgctcgaggtgacaaagcctggacgccgaaaaatcgtgaacgtcaggtctcctttgccctgcgtgcttatgccagcctggcaaccagcgccgacaaaggcgcggtgcgcgataaatcgaaactggggggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61048_sequence/1
type: Sequence
persistent identity: BBa J61048 sequence
display id: BBa_J61048_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z