LOCUS BBa_K539672 5324 bp DNA linear 11-May-2024 DEFINITION promoter (lacI regulated)+RBS+Alss with a RBS (B0032)+ilvC+a RBS (B0032)+ilvD ACCESSION BBa_K539672 VERSION BBa_K539672.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..221 /label=RBS-3\Weak RBS 215..218 /label=RBS misc_feature 209..221 /label=BBa_B0032 RBS 209..221 /label=BBa_B0032 CDS 228..1943 /label=AlsS CDS 228..1943 /label=BBa_K539713 misc_feature 209..1943 /label=BBa_K539627 misc_feature 1..1943 /label=BBa_K539631 RBS 1952..1964 /label=RBS-3\Weak RBS 1958..1961 /label=RBS misc_feature 1952..1964 /label=BBa_B0032 RBS 1952..1964 /label=BBa_B0032 CDS 1971..3446 /label=ilvC coding sequence CDS 1971..3446 /label=BBa_K539711 misc_feature 1952..3446 /label=BBa_K539622 RBS 3455..3467 /label=RBS-3\Weak RBS 3461..3464 /label=RBS misc_feature 3455..3467 /label=BBa_B0032 RBS 3455..3467 /label=BBa_B0032 CDS 3474..5324 /label=ilvD CDS 3474..5324 /label=BBa_K539712 misc_feature 3455..5324 /label=BBa_K539625 misc_feature 1952..5324 /label=BBa_K539641 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg ttgacaaaag 241 caacaaaaga acaaaaatcc cttgtgaaaa acagaggggc ggagcttgtt gttgattgct 301 tagtggagca aggtgtcaca catgtatttg gcattccagg tgcaaaaatt gatgcggtat 361 ttgacgcttt acaagataaa ggacctgaaa ttatcgttgc ccggcacgaa caaaacgcag 421 cattcatggc ccaagcagtc ggccgtttaa ctggaaaacc gggagtcgtg ttagtcacat 481 caggaccggg tgcctctaac ttggcaacag gcctgctgac agcgaacact gaaggagacc 541 ctgtcgttgc gcttgctgga aacgtgatcc gtgcagatcg tttaaaacgg acacatcaat 601 ctttggataa tgcggcgcta ttccagccga ttacaaaata cagtgtagaa gttcaagatg 661 taaaaaatat accggaagct gttacaaatg catttaggat agcgtcagca gggcaggctg 721 gggccgcttt tgtgagcttt ccgcaagatg ttgtgaatga agtcacaaat acgaaaaacg 781 tgcgtgctgt tgcagcgcca aaactcggtc cagcagcaga tgatgcaatc agtgcggcca 841 tagcaaaaat ccaaacagca aaacttcctg tcgttttggt cggcatgaaa ggcggaagac 901 cggaagcaat taaggcggtt cgcaagcttt tgaaaaaggt tcagcttcca tttgttgaaa 961 catatcaagc tgccggtacc ctttccagag atttagagga tcaatatttt ggccgtatcg 1021 gtttgttccg caaccagcct ggcgatttac tgctagagca ggcagatgtt gttctgacga 1081 tcggctatga cccgattgaa tatgatccga aattctggaa tatcaatgga gaccggacaa 1141 ttatccattt agacgagatt atcgctgaca ttgatcatgc ttaccagcct gatcttgaat 1201 tgatcggtga cattccgtcc acgatcaatc atatcgaaca cgatgctgtg aaagtggaat 1261 ttgcagagcg tgagcagaaa atcctttctg atttaaaaca atatatgcat gaaggtgagc 1321 aggtgccagc agattggaaa tcagacagag cgcaccctct tgaaatcgtt aaagagttgc 1381 gtaatgcagt cgatgatcat gttacagtaa cttgcgatat cggttcgcac gccatttgga 1441 tgtcacgtta tttccgcagc tacgagccgt taacattaat gatcagtaac ggtatgcaaa 1501 cactcggcgt tgcgcttcct tgggcaatcg gcgcttcatt ggtgaaaccg ggagaaaaag 1561 tggtttctgt ctctggtgac ggcggtttct tattctcagc aatggaatta gagacagcag 1621 ttcgactaaa agcaccaatt gtacacattg tatggaacga cagcacatat gacatggttg 1681 cattccagca attgaaaaaa tataaccgta catctgcggt cgatttcgga aatatcgata 1741 tcgtgaaata tgcggaaagc ttcggagcaa ctggcttgcg cgtagaatca ccagaccagc 1801 tggcagatgt tctgcgtcaa ggcatgaacg ctgaaggtcc tgtcatcatc gatgtcccgg 1861 ttgactacag tgataacatt aatttagcaa gtgacaagct tccgaaagaa tttggggaac 1921 tcatgaaaac gaaagctctc tagtactaga gtcacacagg aaagtactag atggctaact 1981 acttcaatac actgaatctg cgccagcagc tggcacagct gggcaaatgt cgctttatgg 2041 gccgcgatga atttgccgat ggcgcgagct accttcaggg taaaaaagta gtcatcgtcg 2101 gctgtggcgc acagggtctg aaccagggcc tgaacatgcg tgattctggt ctcgatatct 2161 cctacgctct gcgtaaagaa gcgattgccg agaagcgcgc gtcctggcgt aaagcgaccg 2221 aaaatggttt taaagtgggt acttacgaag aactgatccc acaggcggat ctggtgatta 2281 acctgacgcc ggacaagcag cactctgatg tagtgcgcac cgtacagcca ctgatgaaag 2341 acggcgcggc gctgggctac tcgcacggtt tcaacatcgt cgaagtgggc gagcagatcc 2401 gtaaagatat caccgtagtg atggttgcgc cgaaatgccc aggcaccgaa gtgcgtgaag 2461 agtacaaacg tgggttcggc gtaccgacgc tgattgccgt tcacccggaa aacgatccga 2521 aaggcgaagg catggcgatt gccaaagcct gggcggctgc aaccggtggt caccgtgcgg 2581 gtgtgctgga atcgtccttc gttgcggaag tgaaatctga tctgatgggc gagcaaacca 2641 tcctgtgcgg tatgttgcag gctggctctc tgctgtgctt cgacaagctg gtggaagaag 2701 gtaccgatcc agcatacgca gaaaaactga ttcagttcgg ttgggaaacc atcaccgaag 2761 cactgaaaca gggcggcatc accctgatga tggaccgtct ctctaacccg gcgaaactgc 2821 gtgcttatgc gctttctgaa cagctgaaag agatcatggc acccctgttc cagaaacata 2881 tggacgacat catctccggc gaattttctt ccggtatgat ggcggactgg gccaacgatg 2941 ataagaaact gctgacctgg cgtgaagaga ccggcaaaac cgcgtttgaa accgcgccgc 3001 agtatgaagg caaaatcggc gagcaggagt acttcgataa aggcgtactg atgattgcga 3061 tggtgaaagc gggcgttgaa ctggcgttcg aaaccatggt cgattccggc atcattgaag 3121 agtctgcata ttatgaatca ctgcacgagc tgccgctgat tgccaacacc atcgcccgta 3181 agcgtctgta cgaaatgaac gtggttatct ctgataccgc tgagtacggt aactatctgt 3241 tctcttacgc ttgtgtgccg ttgctgaaac cgtttatggc agagctgcaa ccgggcgacc 3301 tgggtaaagc tattccggaa ggcgcggtag ataacgggca actgcgtgat gtgaacgaag 3361 cgattcgcag ccatgcgatt gagcaggtag gtaagaaact gcgcggctat atgacagata 3421 tgaaacgtat tgctgttgcg ggttaatact agagtcacac aggaaagtac tagatgccta 3481 agtaccgttc cgccaccacc actcatggtc gtaatatggc gggtgctcgt gcgctgtggc 3541 gcgccaccgg aatgaccgac gccgatttcg gtaagccgat tatcgcggtt gtgaactcgt 3601 tcacccaatt tgtaccgggt cacgtccatc tgcgcgatct cggtaaactg gtcgccgaac 3661 aaattgaagc ggctggcggc gttgccaaag agttcaacac cattgcggtg gatgatggga 3721 ttgccatggg ccacgggggg atgctttatt cactgccatc tcgcgaactg atcgctgatt 3781 ccgttgagta tatggtcaac gcccactgcg ccgacgccat ggtctgcatc tctaactgcg 3841 acaaaatcac cccggggatg ctgatggctt ccctgcgcct gaatattccg gtgatctttg 3901 tttccggcgg cccgatggag gccgggaaaa ccaaactttc cgatcagatc atcaagctcg 3961 atctggtcga tgcgatgatc cagggcgcag acccgaaagt atctgactcc cagagcgatc 4021 aggttgaacg ttccgcgtgt ccgacctgcg gttcctgctc cgggatgttt accgctaact 4081 caatgaactg cctgaccgaa gcgctgggcc tgtcgcagcc gggcaacggc tcgctgctgg 4141 caacccacgc cgaccgtaag cagctgttcc ttaatgctgg taaacgcatt gttgaattga 4201 ccaaacgtta ttacgagcaa aacgacgaaa gtgcactgcc gcgtaatatc gccagtaagg 4261 cggcgtttga aaacgccatg acgctggata tcgcgatggg tggatcgact aacaccgtac 4321 ttcacctgct ggcggcggcg caggaagcgg aaatcgactt caccatgagt gatatcgata 4381 agctttcccg caaggttcca cagctgtgta aagttgcgcc gagcacccag aaataccata 4441 tggaagatgt tcaccgtgct ggtggtgtta tcggtattct cggcgaactg gatcgcgcgg 4501 ggttactgaa ccgtgatgtg aaaaacgtac ttggcctgac gttgccgcaa acgctggaac 4561 aatacgacgt tatgctgacc caggatgacg cggtaaaaaa tatgttccgc gcaggtccag 4621 caggcattcg taccacacag gcattctcgc aagattgccg ttgggatacg ctggacgacg 4681 atcgcgccaa tggctgtatc cgctcgctgg aacacgccta cagcaaagac ggcggcctgg 4741 cggtgctcta cggtaacttt gcggaaaacg gctgcatcgt gaaaacggca ggcgtcgatg 4801 acagcatcct caaattcacc ggcccggcga aagtgtacga aagccaggac gatgcggtag 4861 aagcgattct cggcggtaaa gttgtcgccg gagatgtggt agtaattcgc tatgaaggcc 4921 cgaaaggcgg tccggggatg caggaaatgc tctacccaac cagcttcctg aaatcaatgg 4981 gtctcggcaa agcctgtgcg ctgatcaccg acggtcgttt ctctggtggc acctctggtc 5041 tttccatcgg ccacgtctca ccggaagcgg caagcggcgg cagcattggc ctgattgaag 5101 atggtgacct gatcgctatc gacatcccga accgtggcat tcagttacag gtaagcgatg 5161 ccgaactggc ggcgcgtcgt gaagcgcagg acgctcgagg tgacaaagcc tggacgccga 5221 aaaatcgtga acgtcaggtc tcctttgccc tgcgtgctta tgccagcctg gcaaccagcg 5281 ccgacaaagg cgcggtgcgc gataaatcga aactgggggg ttaa //