##gff-version 3
##sequence-region BBa_K539673 1 4245
BBa_K539673	.	engineered_region	1	3373	.	+	0	ID=BBa_K539641;Name=BBa_K539641
BBa_K539673	.	engineered_region	1	1495	.	+	0	ID=BBa_K539622;Name=BBa_K539622;Parent=BBa_K539641
BBa_K539673	.	CDS	20	1495	.	+	0	ID=BBa_K539711;Name=BBa_K539711;Parent=BBa_K539622
BBa_K539673	.	CDS	20	1495	.	+	0	ID=annotation2136206;Name=ilvC coding sequence;Parent=BBa_K539711
BBa_K539673	.	ribosome_entry_site	1	13	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K539622
BBa_K539673	.	engineered_region	1	13	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K539673	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K539673	.	ribosome_entry_site	1	13	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K539673	.	engineered_region	1504	3373	.	+	0	ID=BBa_K539625;Name=BBa_K539625;Parent=BBa_K539641
BBa_K539673	.	CDS	1523	3373	.	+	0	ID=BBa_K539712;Name=BBa_K539712;Parent=BBa_K539625
BBa_K539673	.	CDS	1523	3373	.	+	0	ID=annotation2136209;Name=ilvD;Parent=BBa_K539712
BBa_K539673	.	ribosome_entry_site	1504	1516	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K539625
BBa_K539673	.	engineered_region	1504	1516	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K539673	.	ribosome_entry_site	1510	1513	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K539673	.	ribosome_entry_site	1504	1516	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K539673	.	engineered_region	3382	4245	.	+	0	ID=BBa_K332033;Name=BBa_K332033
BBa_K539673	.	mature_transcript_region	3382	3433	.	+	0	ID=BBa_K115002;Name=BBa_K115002;Parent=BBa_K332033
BBa_K539673	.	stem_loop	3382	3399	.	+	0	ID=annotation1966930;Name=stem_loop;Parent=BBa_K115002
BBa_K539673	.	sequence_alteration	3404	3409	.	+	0	ID=annotation1966899;Name=scar adaptation;Parent=BBa_K115002
BBa_K539673	.	stem_loop	3405	3433	.	+	0	ID=annotation1966931;Name=predicted stem-loop extending to one position before start codon;Parent=BBa_K115002
BBa_K539673	.	ribosome_entry_site	3428	3433	.	+	0	ID=annotation1966897;Name=rbs;Parent=BBa_K115002
BBa_K539673	.	terminator	4133	4245	.	+	0	ID=BBa_J61048;Name=BBa_J61048;Parent=BBa_K332033
BBa_K539673	.	CDS	3440	4124	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K332033
BBa_K539673	.	engineered_tag	4100	4124	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K539673	.	CDS	3443	4059	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K539673	.	tag	4060	4093	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
>BBa_K539673
tcacacaggaaagtactagatggctaactacttcaatacactgaatctgcgccagcagctggcacagctgggcaaatgt
cgctttatgggccgcgatgaatttgccgatggcgcgagctaccttcagggtaaaaaagtagtcatcgtcggctgtggcg
cacagggtctgaaccagggcctgaacatgcgtgattctggtctcgatatctcctacgctctgcgtaaagaagcgattgc
cgagaagcgcgcgtcctggcgtaaagcgaccgaaaatggttttaaagtgggtacttacgaagaactgatcccacaggcg
gatctggtgattaacctgacgccggacaagcagcactctgatgtagtgcgcaccgtacagccactgatgaaagacggcg
cggcgctgggctactcgcacggtttcaacatcgtcgaagtgggcgagcagatccgtaaagatatcaccgtagtgatggt
tgcgccgaaatgcccaggcaccgaagtgcgtgaagagtacaaacgtgggttcggcgtaccgacgctgattgccgttcac
ccggaaaacgatccgaaaggcgaaggcatggcgattgccaaagcctgggcggctgcaaccggtggtcaccgtgcgggtg
tgctggaatcgtccttcgttgcggaagtgaaatctgatctgatgggcgagcaaaccatcctgtgcggtatgttgcaggc
tggctctctgctgtgcttcgacaagctggtggaagaaggtaccgatccagcatacgcagaaaaactgattcagttcggt
tgggaaaccatcaccgaagcactgaaacagggcggcatcaccctgatgatggaccgtctctctaacccggcgaaactgc
gtgcttatgcgctttctgaacagctgaaagagatcatggcacccctgttccagaaacatatggacgacatcatctccgg
cgaattttcttccggtatgatggcggactgggccaacgatgataagaaactgctgacctggcgtgaagagaccggcaaa
accgcgtttgaaaccgcgccgcagtatgaaggcaaaatcggcgagcaggagtacttcgataaaggcgtactgatgattg
cgatggtgaaagcgggcgttgaactggcgttcgaaaccatggtcgattccggcatcattgaagagtctgcatattatga
atcactgcacgagctgccgctgattgccaacaccatcgcccgtaagcgtctgtacgaaatgaacgtggttatctctgat
accgctgagtacggtaactatctgttctcttacgcttgtgtgccgttgctgaaaccgtttatggcagagctgcaaccgg
gcgacctgggtaaagctattccggaaggcgcggtagataacgggcaactgcgtgatgtgaacgaagcgattcgcagcca
tgcgattgagcaggtaggtaagaaactgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaatactag
agtcacacaggaaagtactagatgcctaagtaccgttccgccaccaccactcatggtcgtaatatggcgggtgctcgtg
cgctgtggcgcgccaccggaatgaccgacgccgatttcggtaagccgattatcgcggttgtgaactcgttcacccaatt
tgtaccgggtcacgtccatctgcgcgatctcggtaaactggtcgccgaacaaattgaagcggctggcggcgttgccaaa
gagttcaacaccattgcggtggatgatgggattgccatgggccacggggggatgctttattcactgccatctcgcgaac
tgatcgctgattccgttgagtatatggtcaacgcccactgcgccgacgccatggtctgcatctctaactgcgacaaaat
caccccggggatgctgatggcttccctgcgcctgaatattccggtgatctttgtttccggcggcccgatggaggccggg
aaaaccaaactttccgatcagatcatcaagctcgatctggtcgatgcgatgatccagggcgcagacccgaaagtatctg
actcccagagcgatcaggttgaacgttccgcgtgtccgacctgcggttcctgctccgggatgtttaccgctaactcaat
gaactgcctgaccgaagcgctgggcctgtcgcagccgggcaacggctcgctgctggcaacccacgccgaccgtaagcag
ctgttccttaatgctggtaaacgcattgttgaattgaccaaacgttattacgagcaaaacgacgaaagtgcactgccgc
gtaatatcgccagtaaggcggcgtttgaaaacgccatgacgctggatatcgcgatgggtggatcgactaacaccgtact
tcacctgctggcggcggcgcaggaagcggaaatcgacttcaccatgagtgatatcgataagctttcccgcaaggttcca
cagctgtgtaaagttgcgccgagcacccagaaataccatatggaagatgttcaccgtgctggtggtgttatcggtattc
tcggcgaactggatcgcgcggggttactgaaccgtgatgtgaaaaacgtacttggcctgacgttgccgcaaacgctgga
acaatacgacgttatgctgacccaggatgacgcggtaaaaaatatgttccgcgcaggtccagcaggcattcgtaccaca
caggcattctcgcaagattgccgttgggatacgctggacgacgatcgcgccaatggctgtatccgctcgctggaacacg
cctacagcaaagacggcggcctggcggtgctctacggtaactttgcggaaaacggctgcatcgtgaaaacggcaggcgt
cgatgacagcatcctcaaattcaccggcccggcgaaagtgtacgaaagccaggacgatgcggtagaagcgattctcggc
ggtaaagttgtcgccggagatgtggtagtaattcgctatgaaggcccgaaaggcggtccggggatgcaggaaatgctct
acccaaccagcttcctgaaatcaatgggtctcggcaaagcctgtgcgctgatcaccgacggtcgtttctctggtggcac
ctctggtctttccatcggccacgtctcaccggaagcggcaagcggcggcagcattggcctgattgaagatggtgacctg
atcgctatcgacatcccgaaccgtggcattcagttacaggtaagcgatgccgaactggcggcgcgtcgtgaagcgcagg
acgctcgaggtgacaaagcctggacgccgaaaaatcgtgaacgtcaggtctcctttgccctgcgtgcttatgccagcct
ggcaaccagcgccgacaaaggcgcggtgcgcgataaatcgaaactggggggttaatactagagggacaagcaatgcttg
ccttgaatagtaacttttgaatagtgattcaggaggtactagatgtccagattagataaaagtaaagtgattaacagcg
cattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcc
tacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatact
cacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaa
gtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagc
ctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgc
gtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattac
gacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggatt
agaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatag
tgctagtgtagatcactactagagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggttt
ttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa