LOCUS BBa_K539674 4249 bp DNA linear 22-Dec-2024 DEFINITION a RBS (B0030)+ilvC+a RBS (B0030)+ilvD with RNA thermometer+tetR+ter minator ACCESSION BBa_K539674 VERSION BBa_K539674.1 FEATURES Location/Qualifiers RBS 1..15 /label=RBS-1\Strong RBS 8..12 /label=RBS misc_feature 1..15 /label=BBa_B0030 RBS 1..15 /label=BBa_B0030 CDS 22..1497 /label=ilvC coding sequence CDS 22..1497 /label=BBa_K539711 misc_feature 1..1497 /label=BBa_K539621 RBS 1506..1520 /label=RBS-1\Strong RBS 1513..1517 /label=RBS misc_feature 1506..1520 /label=BBa_B0030 RBS 1506..1520 /label=BBa_B0030 CDS 1527..3377 /label=ilvD CDS 1527..3377 /label=BBa_K539712 misc_feature 1506..3377 /label=BBa_K539624 misc_feature 1..3377 /label=BBa_K539642 RBS 3432..3437 /label=rbs misc_feature 3408..3413 /label=scar adaptation stem_loop 3386..3403 /label=stem_loop stem_loop 3409..3437 /label=predicted stem-loop extending to one position before start codon misc_feature 3386..3437 /label=BBa_K115002 CDS 3447..4063 /label=tetR misc_feature 4064..4097 /label=SsrA misc_feature 4104..4128 /label=Help:Barcodes CDS 3444..4128 /label=BBa_C0040 terminator 4137..4249 /label=BBa_J61048 misc_feature 3386..4249 /label=BBa_K332033 ORIGIN 1 attaaagagg agaaatacta gatggctaac tacttcaata cactgaatct gcgccagcag 61 ctggcacagc tgggcaaatg tcgctttatg ggccgcgatg aatttgccga tggcgcgagc 121 taccttcagg gtaaaaaagt agtcatcgtc ggctgtggcg cacagggtct gaaccagggc 181 ctgaacatgc gtgattctgg tctcgatatc tcctacgctc tgcgtaaaga agcgattgcc 241 gagaagcgcg cgtcctggcg taaagcgacc gaaaatggtt ttaaagtggg tacttacgaa 301 gaactgatcc cacaggcgga tctggtgatt aacctgacgc cggacaagca gcactctgat 361 gtagtgcgca ccgtacagcc actgatgaaa gacggcgcgg cgctgggcta ctcgcacggt 421 ttcaacatcg tcgaagtggg cgagcagatc cgtaaagata tcaccgtagt gatggttgcg 481 ccgaaatgcc caggcaccga agtgcgtgaa gagtacaaac gtgggttcgg cgtaccgacg 541 ctgattgccg ttcacccgga aaacgatccg aaaggcgaag gcatggcgat tgccaaagcc 601 tgggcggctg caaccggtgg tcaccgtgcg ggtgtgctgg aatcgtcctt cgttgcggaa 661 gtgaaatctg atctgatggg cgagcaaacc atcctgtgcg gtatgttgca ggctggctct 721 ctgctgtgct tcgacaagct ggtggaagaa ggtaccgatc cagcatacgc agaaaaactg 781 attcagttcg gttgggaaac catcaccgaa gcactgaaac agggcggcat caccctgatg 841 atggaccgtc tctctaaccc ggcgaaactg cgtgcttatg cgctttctga acagctgaaa 901 gagatcatgg cacccctgtt ccagaaacat atggacgaca tcatctccgg cgaattttct 961 tccggtatga tggcggactg ggccaacgat gataagaaac tgctgacctg gcgtgaagag 1021 accggcaaaa ccgcgtttga aaccgcgccg cagtatgaag gcaaaatcgg cgagcaggag 1081 tacttcgata aaggcgtact gatgattgcg atggtgaaag cgggcgttga actggcgttc 1141 gaaaccatgg tcgattccgg catcattgaa gagtctgcat attatgaatc actgcacgag 1201 ctgccgctga ttgccaacac catcgcccgt aagcgtctgt acgaaatgaa cgtggttatc 1261 tctgataccg ctgagtacgg taactatctg ttctcttacg cttgtgtgcc gttgctgaaa 1321 ccgtttatgg cagagctgca accgggcgac ctgggtaaag ctattccgga aggcgcggta 1381 gataacgggc aactgcgtga tgtgaacgaa gcgattcgca gccatgcgat tgagcaggta 1441 ggtaagaaac tgcgcggcta tatgacagat atgaaacgta ttgctgttgc gggttaatac 1501 tagagattaa agaggagaaa tactagatgc ctaagtaccg ttccgccacc accactcatg 1561 gtcgtaatat ggcgggtgct cgtgcgctgt ggcgcgccac cggaatgacc gacgccgatt 1621 tcggtaagcc gattatcgcg gttgtgaact cgttcaccca atttgtaccg ggtcacgtcc 1681 atctgcgcga tctcggtaaa ctggtcgccg aacaaattga agcggctggc ggcgttgcca 1741 aagagttcaa caccattgcg gtggatgatg ggattgccat gggccacggg gggatgcttt 1801 attcactgcc atctcgcgaa ctgatcgctg attccgttga gtatatggtc aacgcccact 1861 gcgccgacgc catggtctgc atctctaact gcgacaaaat caccccgggg atgctgatgg 1921 cttccctgcg cctgaatatt ccggtgatct ttgtttccgg cggcccgatg gaggccggga 1981 aaaccaaact ttccgatcag atcatcaagc tcgatctggt cgatgcgatg atccagggcg 2041 cagacccgaa agtatctgac tcccagagcg atcaggttga acgttccgcg tgtccgacct 2101 gcggttcctg ctccgggatg tttaccgcta actcaatgaa ctgcctgacc gaagcgctgg 2161 gcctgtcgca gccgggcaac ggctcgctgc tggcaaccca cgccgaccgt aagcagctgt 2221 tccttaatgc tggtaaacgc attgttgaat tgaccaaacg ttattacgag caaaacgacg 2281 aaagtgcact gccgcgtaat atcgccagta aggcggcgtt tgaaaacgcc atgacgctgg 2341 atatcgcgat gggtggatcg actaacaccg tacttcacct gctggcggcg gcgcaggaag 2401 cggaaatcga cttcaccatg agtgatatcg ataagctttc ccgcaaggtt ccacagctgt 2461 gtaaagttgc gccgagcacc cagaaatacc atatggaaga tgttcaccgt gctggtggtg 2521 ttatcggtat tctcggcgaa ctggatcgcg cggggttact gaaccgtgat gtgaaaaacg 2581 tacttggcct gacgttgccg caaacgctgg aacaatacga cgttatgctg acccaggatg 2641 acgcggtaaa aaatatgttc cgcgcaggtc cagcaggcat tcgtaccaca caggcattct 2701 cgcaagattg ccgttgggat acgctggacg acgatcgcgc caatggctgt atccgctcgc 2761 tggaacacgc ctacagcaaa gacggcggcc tggcggtgct ctacggtaac tttgcggaaa 2821 acggctgcat cgtgaaaacg gcaggcgtcg atgacagcat cctcaaattc accggcccgg 2881 cgaaagtgta cgaaagccag gacgatgcgg tagaagcgat tctcggcggt aaagttgtcg 2941 ccggagatgt ggtagtaatt cgctatgaag gcccgaaagg cggtccgggg atgcaggaaa 3001 tgctctaccc aaccagcttc ctgaaatcaa tgggtctcgg caaagcctgt gcgctgatca 3061 ccgacggtcg tttctctggt ggcacctctg gtctttccat cggccacgtc tcaccggaag 3121 cggcaagcgg cggcagcatt ggcctgattg aagatggtga cctgatcgct atcgacatcc 3181 cgaaccgtgg cattcagtta caggtaagcg atgccgaact ggcggcgcgt cgtgaagcgc 3241 aggacgctcg aggtgacaaa gcctggacgc cgaaaaatcg tgaacgtcag gtctcctttg 3301 ccctgcgtgc ttatgccagc ctggcaacca gcgccgacaa aggcgcggtg cgcgataaat 3361 cgaaactggg gggttaatac tagagggaca agcaatgctt gccttgaata gtaacttttg 3421 aatagtgatt caggaggtac tagatgtcca gattagataa aagtaaagtg attaacagcg 3481 cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga 3541 agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg 3601 acgccttagc cattgagatg ttagataggc accatactca cttttgccct ttagaagggg 3661 aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc 3721 atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc 3781 tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg 3841 cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag 3901 tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag 3961 ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga 4021 tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtccgctgca aacgacgaaa 4081 actacgcttt agtagcttaa taacactgat agtgctagtg tagatcacta ctagagccgg 4141 cttatcggtc agtttcacct gatttacgta aaaacccgct tcggcgggtt tttgcttttg 4201 gaggggcaga aagatgaatg actgtccacg acgctatacc caaaagaaa //