##gff-version 3
##sequence-region BBa_K539674 1 4249
BBa_K539674	.	engineered_region	3386	4249	.	+	0	ID=BBa_K332033;Name=BBa_K332033
BBa_K539674	.	terminator	4137	4249	.	+	0	ID=BBa_J61048;Name=BBa_J61048;Parent=BBa_K332033
BBa_K539674	.	CDS	3444	4128	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K332033
BBa_K539674	.	tag	4064	4097	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K539674	.	engineered_tag	4104	4128	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K539674	.	CDS	3447	4063	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K539674	.	mature_transcript_region	3386	3437	.	+	0	ID=BBa_K115002;Name=BBa_K115002;Parent=BBa_K332033
BBa_K539674	.	sequence_alteration	3408	3413	.	+	0	ID=annotation1966899;Name=scar adaptation;Parent=BBa_K115002
BBa_K539674	.	stem_loop	3409	3437	.	+	0	ID=annotation1966931;Name=predicted stem-loop extending to one position before start codon;Parent=BBa_K115002
BBa_K539674	.	stem_loop	3386	3403	.	+	0	ID=annotation1966930;Name=stem_loop;Parent=BBa_K115002
BBa_K539674	.	ribosome_entry_site	3432	3437	.	+	0	ID=annotation1966897;Name=rbs;Parent=BBa_K115002
BBa_K539674	.	engineered_region	1	3377	.	+	0	ID=BBa_K539642;Name=BBa_K539642
BBa_K539674	.	engineered_region	1506	3377	.	+	0	ID=BBa_K539624;Name=BBa_K539624;Parent=BBa_K539642
BBa_K539674	.	CDS	1527	3377	.	+	0	ID=BBa_K539712;Name=BBa_K539712;Parent=BBa_K539624
BBa_K539674	.	CDS	1527	3377	.	+	0	ID=annotation2136209;Name=ilvD;Parent=BBa_K539712
BBa_K539674	.	ribosome_entry_site	1506	1520	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K539624
BBa_K539674	.	ribosome_entry_site	1506	1520	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K539674	.	engineered_region	1506	1520	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K539674	.	ribosome_entry_site	1513	1517	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K539674	.	engineered_region	1	1497	.	+	0	ID=BBa_K539621;Name=BBa_K539621;Parent=BBa_K539642
BBa_K539674	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K539621
BBa_K539674	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K539674	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K539674	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K539674	.	CDS	22	1497	.	+	0	ID=BBa_K539711;Name=BBa_K539711;Parent=BBa_K539621
BBa_K539674	.	CDS	22	1497	.	+	0	ID=annotation2136206;Name=ilvC coding sequence;Parent=BBa_K539711
>BBa_K539674
attaaagaggagaaatactagatggctaactacttcaatacactgaatctgcgccagcagctggcacagctgggcaaat
gtcgctttatgggccgcgatgaatttgccgatggcgcgagctaccttcagggtaaaaaagtagtcatcgtcggctgtgg
cgcacagggtctgaaccagggcctgaacatgcgtgattctggtctcgatatctcctacgctctgcgtaaagaagcgatt
gccgagaagcgcgcgtcctggcgtaaagcgaccgaaaatggttttaaagtgggtacttacgaagaactgatcccacagg
cggatctggtgattaacctgacgccggacaagcagcactctgatgtagtgcgcaccgtacagccactgatgaaagacgg
cgcggcgctgggctactcgcacggtttcaacatcgtcgaagtgggcgagcagatccgtaaagatatcaccgtagtgatg
gttgcgccgaaatgcccaggcaccgaagtgcgtgaagagtacaaacgtgggttcggcgtaccgacgctgattgccgttc
acccggaaaacgatccgaaaggcgaaggcatggcgattgccaaagcctgggcggctgcaaccggtggtcaccgtgcggg
tgtgctggaatcgtccttcgttgcggaagtgaaatctgatctgatgggcgagcaaaccatcctgtgcggtatgttgcag
gctggctctctgctgtgcttcgacaagctggtggaagaaggtaccgatccagcatacgcagaaaaactgattcagttcg
gttgggaaaccatcaccgaagcactgaaacagggcggcatcaccctgatgatggaccgtctctctaacccggcgaaact
gcgtgcttatgcgctttctgaacagctgaaagagatcatggcacccctgttccagaaacatatggacgacatcatctcc
ggcgaattttcttccggtatgatggcggactgggccaacgatgataagaaactgctgacctggcgtgaagagaccggca
aaaccgcgtttgaaaccgcgccgcagtatgaaggcaaaatcggcgagcaggagtacttcgataaaggcgtactgatgat
tgcgatggtgaaagcgggcgttgaactggcgttcgaaaccatggtcgattccggcatcattgaagagtctgcatattat
gaatcactgcacgagctgccgctgattgccaacaccatcgcccgtaagcgtctgtacgaaatgaacgtggttatctctg
ataccgctgagtacggtaactatctgttctcttacgcttgtgtgccgttgctgaaaccgtttatggcagagctgcaacc
gggcgacctgggtaaagctattccggaaggcgcggtagataacgggcaactgcgtgatgtgaacgaagcgattcgcagc
catgcgattgagcaggtaggtaagaaactgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaatact
agagattaaagaggagaaatactagatgcctaagtaccgttccgccaccaccactcatggtcgtaatatggcgggtgct
cgtgcgctgtggcgcgccaccggaatgaccgacgccgatttcggtaagccgattatcgcggttgtgaactcgttcaccc
aatttgtaccgggtcacgtccatctgcgcgatctcggtaaactggtcgccgaacaaattgaagcggctggcggcgttgc
caaagagttcaacaccattgcggtggatgatgggattgccatgggccacggggggatgctttattcactgccatctcgc
gaactgatcgctgattccgttgagtatatggtcaacgcccactgcgccgacgccatggtctgcatctctaactgcgaca
aaatcaccccggggatgctgatggcttccctgcgcctgaatattccggtgatctttgtttccggcggcccgatggaggc
cgggaaaaccaaactttccgatcagatcatcaagctcgatctggtcgatgcgatgatccagggcgcagacccgaaagta
tctgactcccagagcgatcaggttgaacgttccgcgtgtccgacctgcggttcctgctccgggatgtttaccgctaact
caatgaactgcctgaccgaagcgctgggcctgtcgcagccgggcaacggctcgctgctggcaacccacgccgaccgtaa
gcagctgttccttaatgctggtaaacgcattgttgaattgaccaaacgttattacgagcaaaacgacgaaagtgcactg
ccgcgtaatatcgccagtaaggcggcgtttgaaaacgccatgacgctggatatcgcgatgggtggatcgactaacaccg
tacttcacctgctggcggcggcgcaggaagcggaaatcgacttcaccatgagtgatatcgataagctttcccgcaaggt
tccacagctgtgtaaagttgcgccgagcacccagaaataccatatggaagatgttcaccgtgctggtggtgttatcggt
attctcggcgaactggatcgcgcggggttactgaaccgtgatgtgaaaaacgtacttggcctgacgttgccgcaaacgc
tggaacaatacgacgttatgctgacccaggatgacgcggtaaaaaatatgttccgcgcaggtccagcaggcattcgtac
cacacaggcattctcgcaagattgccgttgggatacgctggacgacgatcgcgccaatggctgtatccgctcgctggaa
cacgcctacagcaaagacggcggcctggcggtgctctacggtaactttgcggaaaacggctgcatcgtgaaaacggcag
gcgtcgatgacagcatcctcaaattcaccggcccggcgaaagtgtacgaaagccaggacgatgcggtagaagcgattct
cggcggtaaagttgtcgccggagatgtggtagtaattcgctatgaaggcccgaaaggcggtccggggatgcaggaaatg
ctctacccaaccagcttcctgaaatcaatgggtctcggcaaagcctgtgcgctgatcaccgacggtcgtttctctggtg
gcacctctggtctttccatcggccacgtctcaccggaagcggcaagcggcggcagcattggcctgattgaagatggtga
cctgatcgctatcgacatcccgaaccgtggcattcagttacaggtaagcgatgccgaactggcggcgcgtcgtgaagcg
caggacgctcgaggtgacaaagcctggacgccgaaaaatcgtgaacgtcaggtctcctttgccctgcgtgcttatgcca
gcctggcaaccagcgccgacaaaggcgcggtgcgcgataaatcgaaactggggggttaatactagagggacaagcaatg
cttgccttgaatagtaacttttgaatagtgattcaggaggtactagatgtccagattagataaaagtaaagtgattaac
agcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagc
agcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcacca
tactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgcttta
ctaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaat
tagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttagg
ttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccatta
ttacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcg
gattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactg
atagtgctagtgtagatcactactagagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgg
gtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa