LOCUS BBa_K539691 6200 bp DNA linear 25-Nov-2024 DEFINITION promoter(lacI regulated)+Alss+ilvC+ilvD(each preceded by own RBS)and RNA thermometer+terminator ACCESSION BBa_K539691 VERSION BBa_K539691.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..221 /label=RBS-3\Weak RBS 215..218 /label=RBS misc_feature 209..221 /label=BBa_B0032 RBS 209..221 /label=BBa_B0032 CDS 228..1943 /label=AlsS CDS 228..1943 /label=BBa_K539713 misc_feature 209..1943 /label=BBa_K539627 misc_feature 1..1943 /label=BBa_K539631 RBS 1952..1966 /label=RBS-1\Strong RBS 1959..1963 /label=RBS misc_feature 1952..1966 /label=BBa_B0030 RBS 1952..1966 /label=BBa_B0030 CDS 1973..3448 /label=ilvC coding sequence CDS 1973..3448 /label=BBa_K539711 misc_feature 1952..3448 /label=BBa_K539621 misc_feature 1..3448 /label=BBa_K539651 RBS 3457..3471 /label=RBS-1\Strong RBS 3464..3468 /label=RBS misc_feature 3457..3471 /label=BBa_B0030 RBS 3457..3471 /label=BBa_B0030 CDS 3478..5328 /label=ilvD CDS 3478..5328 /label=BBa_K539712 misc_feature 3457..5328 /label=BBa_K539624 RBS 5383..5388 /label=rbs misc_feature 5359..5364 /label=scar adaptation stem_loop 5337..5354 /label=stem_loop stem_loop 5360..5388 /label=predicted stem-loop extending to one position before start codon misc_feature 5337..5388 /label=BBa_K115002 CDS 5398..6014 /label=tetR misc_feature 6015..6048 /label=SsrA misc_feature 6055..6079 /label=Help:Barcodes CDS 5395..6079 /label=BBa_C0040 terminator 6088..6200 /label=BBa_J61048 misc_feature 5337..6200 /label=BBa_K332033 misc_feature 3457..6200 /label=BBa_K539653 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg ttgacaaaag 241 caacaaaaga acaaaaatcc cttgtgaaaa acagaggggc ggagcttgtt gttgattgct 301 tagtggagca aggtgtcaca catgtatttg gcattccagg tgcaaaaatt gatgcggtat 361 ttgacgcttt acaagataaa ggacctgaaa ttatcgttgc ccggcacgaa caaaacgcag 421 cattcatggc ccaagcagtc ggccgtttaa ctggaaaacc gggagtcgtg ttagtcacat 481 caggaccggg tgcctctaac ttggcaacag gcctgctgac agcgaacact gaaggagacc 541 ctgtcgttgc gcttgctgga aacgtgatcc gtgcagatcg tttaaaacgg acacatcaat 601 ctttggataa tgcggcgcta ttccagccga ttacaaaata cagtgtagaa gttcaagatg 661 taaaaaatat accggaagct gttacaaatg catttaggat agcgtcagca gggcaggctg 721 gggccgcttt tgtgagcttt ccgcaagatg ttgtgaatga agtcacaaat acgaaaaacg 781 tgcgtgctgt tgcagcgcca aaactcggtc cagcagcaga tgatgcaatc agtgcggcca 841 tagcaaaaat ccaaacagca aaacttcctg tcgttttggt cggcatgaaa ggcggaagac 901 cggaagcaat taaggcggtt cgcaagcttt tgaaaaaggt tcagcttcca tttgttgaaa 961 catatcaagc tgccggtacc ctttccagag atttagagga tcaatatttt ggccgtatcg 1021 gtttgttccg caaccagcct ggcgatttac tgctagagca ggcagatgtt gttctgacga 1081 tcggctatga cccgattgaa tatgatccga aattctggaa tatcaatgga gaccggacaa 1141 ttatccattt agacgagatt atcgctgaca ttgatcatgc ttaccagcct gatcttgaat 1201 tgatcggtga cattccgtcc acgatcaatc atatcgaaca cgatgctgtg aaagtggaat 1261 ttgcagagcg tgagcagaaa atcctttctg atttaaaaca atatatgcat gaaggtgagc 1321 aggtgccagc agattggaaa tcagacagag cgcaccctct tgaaatcgtt aaagagttgc 1381 gtaatgcagt cgatgatcat gttacagtaa cttgcgatat cggttcgcac gccatttgga 1441 tgtcacgtta tttccgcagc tacgagccgt taacattaat gatcagtaac ggtatgcaaa 1501 cactcggcgt tgcgcttcct tgggcaatcg gcgcttcatt ggtgaaaccg ggagaaaaag 1561 tggtttctgt ctctggtgac ggcggtttct tattctcagc aatggaatta gagacagcag 1621 ttcgactaaa agcaccaatt gtacacattg tatggaacga cagcacatat gacatggttg 1681 cattccagca attgaaaaaa tataaccgta catctgcggt cgatttcgga aatatcgata 1741 tcgtgaaata tgcggaaagc ttcggagcaa ctggcttgcg cgtagaatca ccagaccagc 1801 tggcagatgt tctgcgtcaa ggcatgaacg ctgaaggtcc tgtcatcatc gatgtcccgg 1861 ttgactacag tgataacatt aatttagcaa gtgacaagct tccgaaagaa tttggggaac 1921 tcatgaaaac gaaagctctc tagtactaga gattaaagag gagaaatact agatggctaa 1981 ctacttcaat acactgaatc tgcgccagca gctggcacag ctgggcaaat gtcgctttat 2041 gggccgcgat gaatttgccg atggcgcgag ctaccttcag ggtaaaaaag tagtcatcgt 2101 cggctgtggc gcacagggtc tgaaccaggg cctgaacatg cgtgattctg gtctcgatat 2161 ctcctacgct ctgcgtaaag aagcgattgc cgagaagcgc gcgtcctggc gtaaagcgac 2221 cgaaaatggt tttaaagtgg gtacttacga agaactgatc ccacaggcgg atctggtgat 2281 taacctgacg ccggacaagc agcactctga tgtagtgcgc accgtacagc cactgatgaa 2341 agacggcgcg gcgctgggct actcgcacgg tttcaacatc gtcgaagtgg gcgagcagat 2401 ccgtaaagat atcaccgtag tgatggttgc gccgaaatgc ccaggcaccg aagtgcgtga 2461 agagtacaaa cgtgggttcg gcgtaccgac gctgattgcc gttcacccgg aaaacgatcc 2521 gaaaggcgaa ggcatggcga ttgccaaagc ctgggcggct gcaaccggtg gtcaccgtgc 2581 gggtgtgctg gaatcgtcct tcgttgcgga agtgaaatct gatctgatgg gcgagcaaac 2641 catcctgtgc ggtatgttgc aggctggctc tctgctgtgc ttcgacaagc tggtggaaga 2701 aggtaccgat ccagcatacg cagaaaaact gattcagttc ggttgggaaa ccatcaccga 2761 agcactgaaa cagggcggca tcaccctgat gatggaccgt ctctctaacc cggcgaaact 2821 gcgtgcttat gcgctttctg aacagctgaa agagatcatg gcacccctgt tccagaaaca 2881 tatggacgac atcatctccg gcgaattttc ttccggtatg atggcggact gggccaacga 2941 tgataagaaa ctgctgacct ggcgtgaaga gaccggcaaa accgcgtttg aaaccgcgcc 3001 gcagtatgaa ggcaaaatcg gcgagcagga gtacttcgat aaaggcgtac tgatgattgc 3061 gatggtgaaa gcgggcgttg aactggcgtt cgaaaccatg gtcgattccg gcatcattga 3121 agagtctgca tattatgaat cactgcacga gctgccgctg attgccaaca ccatcgcccg 3181 taagcgtctg tacgaaatga acgtggttat ctctgatacc gctgagtacg gtaactatct 3241 gttctcttac gcttgtgtgc cgttgctgaa accgtttatg gcagagctgc aaccgggcga 3301 cctgggtaaa gctattccgg aaggcgcggt agataacggg caactgcgtg atgtgaacga 3361 agcgattcgc agccatgcga ttgagcaggt aggtaagaaa ctgcgcggct atatgacaga 3421 tatgaaacgt attgctgttg cgggttaata ctagagatta aagaggagaa atactagatg 3481 cctaagtacc gttccgccac caccactcat ggtcgtaata tggcgggtgc tcgtgcgctg 3541 tggcgcgcca ccggaatgac cgacgccgat ttcggtaagc cgattatcgc ggttgtgaac 3601 tcgttcaccc aatttgtacc gggtcacgtc catctgcgcg atctcggtaa actggtcgcc 3661 gaacaaattg aagcggctgg cggcgttgcc aaagagttca acaccattgc ggtggatgat 3721 gggattgcca tgggccacgg ggggatgctt tattcactgc catctcgcga actgatcgct 3781 gattccgttg agtatatggt caacgcccac tgcgccgacg ccatggtctg catctctaac 3841 tgcgacaaaa tcaccccggg gatgctgatg gcttccctgc gcctgaatat tccggtgatc 3901 tttgtttccg gcggcccgat ggaggccggg aaaaccaaac tttccgatca gatcatcaag 3961 ctcgatctgg tcgatgcgat gatccagggc gcagacccga aagtatctga ctcccagagc 4021 gatcaggttg aacgttccgc gtgtccgacc tgcggttcct gctccgggat gtttaccgct 4081 aactcaatga actgcctgac cgaagcgctg ggcctgtcgc agccgggcaa cggctcgctg 4141 ctggcaaccc acgccgaccg taagcagctg ttccttaatg ctggtaaacg cattgttgaa 4201 ttgaccaaac gttattacga gcaaaacgac gaaagtgcac tgccgcgtaa tatcgccagt 4261 aaggcggcgt ttgaaaacgc catgacgctg gatatcgcga tgggtggatc gactaacacc 4321 gtacttcacc tgctggcggc ggcgcaggaa gcggaaatcg acttcaccat gagtgatatc 4381 gataagcttt cccgcaaggt tccacagctg tgtaaagttg cgccgagcac ccagaaatac 4441 catatggaag atgttcaccg tgctggtggt gttatcggta ttctcggcga actggatcgc 4501 gcggggttac tgaaccgtga tgtgaaaaac gtacttggcc tgacgttgcc gcaaacgctg 4561 gaacaatacg acgttatgct gacccaggat gacgcggtaa aaaatatgtt ccgcgcaggt 4621 ccagcaggca ttcgtaccac acaggcattc tcgcaagatt gccgttggga tacgctggac 4681 gacgatcgcg ccaatggctg tatccgctcg ctggaacacg cctacagcaa agacggcggc 4741 ctggcggtgc tctacggtaa ctttgcggaa aacggctgca tcgtgaaaac ggcaggcgtc 4801 gatgacagca tcctcaaatt caccggcccg gcgaaagtgt acgaaagcca ggacgatgcg 4861 gtagaagcga ttctcggcgg taaagttgtc gccggagatg tggtagtaat tcgctatgaa 4921 ggcccgaaag gcggtccggg gatgcaggaa atgctctacc caaccagctt cctgaaatca 4981 atgggtctcg gcaaagcctg tgcgctgatc accgacggtc gtttctctgg tggcacctct 5041 ggtctttcca tcggccacgt ctcaccggaa gcggcaagcg gcggcagcat tggcctgatt 5101 gaagatggtg acctgatcgc tatcgacatc ccgaaccgtg gcattcagtt acaggtaagc 5161 gatgccgaac tggcggcgcg tcgtgaagcg caggacgctc gaggtgacaa agcctggacg 5221 ccgaaaaatc gtgaacgtca ggtctccttt gccctgcgtg cttatgccag cctggcaacc 5281 agcgccgaca aaggcgcggt gcgcgataaa tcgaaactgg ggggttaata ctagagggac 5341 aagcaatgct tgccttgaat agtaactttt gaatagtgat tcaggaggta ctagatgtcc 5401 agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc 5461 gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat 5521 tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg 5581 caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac 5641 gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt 5701 acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa 5761 caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta 5821 ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact 5881 actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca 5941 gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa 6001 tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga 6061 tagtgctagt gtagatcact actagagccg gcttatcggt cagtttcacc tgatttacgt 6121 aaaaacccgc ttcggcgggt ttttgctttt ggaggggcag aaagatgaat gactgtccac 6181 gacgctatac ccaaaagaaa //