##gff-version 3
##sequence-region BBa_K539742 1 1864
BBa_K539742	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K539742	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K539742	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K539742	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K539742	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K539742	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K539742	.	CDS	81	1727	.	+	0	ID=BBa_K539714;Name=BBa_K539714
BBa_K539742	.	CDS	81	1727	.	+	0	ID=annotation2136208;Name=kivd coding sequence;Parent=BBa_K539714
BBa_K539742	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K539742	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K539742	.	terminator	1736	1864	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K539742	.	terminator	1824	1864	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K539742	.	engineered_region	1824	1864	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K539742	.	stem_loop	1831	1850	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K539742	.	polyA_site	1851	1864	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K539742	.	stop_codon	1857	1857	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K539742	.	terminator	1736	1815	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K539742	.	engineered_region	1736	1815	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K539742	.	stem_loop	1747	1790	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K539742
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgtatacagtaggagattacctattagaccgattacacgagttaggaattgaagaaatttttggagtccctggagac
tataacttacaatttttagatcaaattatttcccgcaaggatatgaaatgggtcggaaatgctaatgaattaaatgctt
catatatggctgatggctatgctcgtactaaaaaagctgccgcatttcttacaacctttggagtaggtgaattgagtgc
agttaatggattagcaggaagttacgccgaaaatttaccagtagtagaaatagtgggatcacctacatcaaaagttcaa
aatgaaggaaaatttgttcatcatacgctggctgacggtgattttaaacactttatgaaaatgcacgaacctgttacag
cagctcgaactttactgacagcagaaaatgcaaccgttgaaattgaccgagtactttctgcactattaaaagaaagaaa
acctgtctatatcaacttaccagttgatgttgctgctgcaaaagcagagaaaccctcactccctttgaaaaaagaaaac
tcaacttcaaatacaagtgaccaagagatcttgaacaaaattcaagaaagcttgaaaaatgccaaaaaaccaatcgtga
ttacaggacatgaaataattagttttggcttagaaaaaacagtctctcaatttatttcaaagacaaaactacctattac
gacattaaactttggaaaaagttcagttgatgaagctctcccttcatttttaggaatctataatggtaaactctcagag
cctaatcttaaagtattcgtggaatcagccgacttcatcctgatgcttggagttaaactcacagactcttcaacaggag
ccttcactcatcatttaaatgaaaataaaatgatttcactgaatatagatgaaggaaaaatatttaacgaaagcatcca
aaattttgattttgaatccctcatctcctctctcttagacctaagcgaaatagaatacaaaggaaaatatatcgataaa
aagcaagaagactttgttccatcaaatgcgcttttatcacaagaccgcctatggcaagcagttgaaaacctaactcaaa
gcaatgaaacaatcgttgctgaacaagggacatcattctttggcgcttcatcaattttcttaaaaccaaagagtcattt
tattggtcaacccttatggggatcaattggatatacattcccagcagcattaggaagccaaattgcagataaagaaagc
agacaccttttatttattggtgatggttcacttcaacttacggtgcaagaattaggattagcaatcagagaaaaaatta
atccaatttgctttattatcaataatgatggttatacagtcgaaagagaaattcatggaccaaatcaaagctacaatga
tattccaatgtggaattactcaaaattaccagaatcatttggagcaacagaagaacgagtagtctcgaaaatcgttaga
actgaaaatgaatttgtgtctgtcatgaaagaagctcaagcagatccaaatagaatgtactggattgagttaattttgg
caaaagaagatgcaccaaaagtactgaaaaaaatgggcaaactatttgctgaacaaaataaatcataatactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttata