BBa_K540003
1
BBa_K540003
rcn-OmpR234, device that triggers adherence in response to cobalt.
2011-09-14T11:00:00Z
2015-05-08T01:12:38Z
Assembly of the biobricks BBa_K540001 and BBa_K342003
This device is formed from an assembly of rcn, a promoter whose activity increases with cobalt, and ompR234, which stimulates the curli promoter, triggering adherence. This plasmid can make strains form a biofilm when cobalt is found in a medium.
rcn is also sensitive to nickel, but we have not studied this aspect.
true
false
_706_
0
8419
9
Discontinued
false
BBa_K342003 already includes a RBS, none was added.
false
Alexandre Duprey
component2129038
1
BBa_K342003
component2129037
1
BBa_K540001
annotation2129037
1
BBa_K540001
range2129037
1
1
426
annotation2129038
1
BBa_K342003
range2129038
1
435
1173
BBa_K342003
1
BBa_K342003
OmpR234 protein, with higher effect on Curli promoter
2010-10-23T11:00:00Z
2016-02-12T09:01:32Z
pcr
This part is coding for a mutated protein OmpR. This protein will be phosphorilated by the complex Envz. The phosphorilated protein is an activator of the Curli promoter
false
false
_471_
4206
6842
9
It's complicated
true
enter
false
Nathalie Isorce
BBa_K540001
1
BBa_K540001
rcn, cobalt-sensitive promoter
2011-09-14T11:00:00Z
2015-07-14T12:34:23Z
PCR from E.coli MC4100 genome.
Released HQ 2013
Rcn is a promoter that is activated by cobalt. A repressor is fixed to the promoter by default, and cobalt binds to this repressor, which activates the gene under control of this promoter.
Rcn is also activated by nickel, but we have not characterized this aspect.
false
false
_706_
4206
8419
9
In stock
false
An internal iGEM restriction site had to be mutated by QuickChange mutagenesis.
false
Mathilde Dumond
annotation2129035
1
RcnR
range2129035
1
1
279
BBa_K342003_sequence
1
aggaggtatataatgcaagagaactacaagattctggtggtcgatgacgacatgcgcctgcgtgcgctgctggaacgttatctcaccgaacaaggcttccaggttcgaagcgtcgctaatgcagaacagatggatcgccggctgactcgtgaatctttccatcttatggtactggatttaatgttacctggtgaagatggcttgtcgatttgccgacgtcttcgtagtcagagcaacccgatgccgatcattatggtgacggcgaaaggggaagaagtggaccgtatcgtaggcctggagattggcgctgacgactacattccaaaaccgtttaacccgcgtgaactgctggcccgtatccgtgcggtgctgcgtcgtcaggcgaacgaactgccaggcgcaccgtcacaggaagaggcggtaattgctttcggtaagttcaaacttaacctcggtacgcgcgaaatgttccgcgaagacgagccgatgccgctcaccagcggtgagtttgcggtactgaaggcactggtcagccatccgcgtgagccgctctcccgcgataagctgatgaaccttgcccgtggtcgtgaatattccgcaatggaacgctccatcgacgtgcagatttcgcgtctgcgccgcatggtggaagaagatccagcgcatccgcgttacattcagaccgtctggggtctgggctacgtctttgtaccggacggctctaaagcatgataataat
BBa_K540001_sequence
1
ttattattatttgatatatgaatccagcaccttcagaacgacatccagatcttcttcacgttttagctcatccccctggtgaacgatgtgttccgtcagatgacctttaatcacttcccgcatcagaccgtttaccgcgccacggatagcagcaatctgttgtaaaactgctgcgcattcgtgcggctcgtcgagcattttcttgagcgccacgacctggccctgaatcttactggcacgcgctttcagtttctgtttatcacggattgtatgagacatggcaacacctggttaacaagaatatgaaaaatcatagcactattaatctactggggggtagtatcaggtactgggggggagtagaatcagattgccgaattaatactaagaattattatcatgaccgaatttacaactcttcttcag
BBa_K540003_sequence
1
ttattattatttgatatatgaatccagcaccttcagaacgacatccagatcttcttcacgttttagctcatccccctggtgaacgatgtgttccgtcagatgacctttaatcacttcccgcatcagaccgtttaccgcgccacggatagcagcaatctgttgtaaaactgctgcgcattcgtgcggctcgtcgagcattttcttgagcgccacgacctggccctgaatcttactggcacgcgctttcagtttctgtttatcacggattgtatgagacatggcaacacctggttaacaagaatatgaaaaatcatagcactattaatctactggggggtagtatcaggtactgggggggagtagaatcagattgccgaattaatactaagaattattatcatgaccgaatttacaactcttcttcagtactagagaggaggtatataatgcaagagaactacaagattctggtggtcgatgacgacatgcgcctgcgtgcgctgctggaacgttatctcaccgaacaaggcttccaggttcgaagcgtcgctaatgcagaacagatggatcgccggctgactcgtgaatctttccatcttatggtactggatttaatgttacctggtgaagatggcttgtcgatttgccgacgtcttcgtagtcagagcaacccgatgccgatcattatggtgacggcgaaaggggaagaagtggaccgtatcgtaggcctggagattggcgctgacgactacattccaaaaccgtttaacccgcgtgaactgctggcccgtatccgtgcggtgctgcgtcgtcaggcgaacgaactgccaggcgcaccgtcacaggaagaggcggtaattgctttcggtaagttcaaacttaacctcggtacgcgcgaaatgttccgcgaagacgagccgatgccgctcaccagcggtgagtttgcggtactgaaggcactggtcagccatccgcgtgagccgctctcccgcgataagctgatgaaccttgcccgtggtcgtgaatattccgcaatggaacgctccatcgacgtgcagatttcgcgtctgcgccgcatggtggaagaagatccagcgcatccgcgttacattcagaccgtctggggtctgggctacgtctttgtaccggacggctctaaagcatgataataat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z