BBa_K540003 1 BBa_K540003 rcn-OmpR234, device that triggers adherence in response to cobalt. 2011-09-14T11:00:00Z 2015-05-08T01:12:38Z Assembly of the biobricks BBa_K540001 and BBa_K342003 This device is formed from an assembly of rcn, a promoter whose activity increases with cobalt, and ompR234, which stimulates the curli promoter, triggering adherence. This plasmid can make strains form a biofilm when cobalt is found in a medium. rcn is also sensitive to nickel, but we have not studied this aspect. true false _706_ 0 8419 9 Discontinued false BBa_K342003 already includes a RBS, none was added. false Alexandre Duprey component2129038 1 BBa_K342003 component2129037 1 BBa_K540001 annotation2129037 1 BBa_K540001 range2129037 1 1 426 annotation2129038 1 BBa_K342003 range2129038 1 435 1173 BBa_K342003 1 BBa_K342003 OmpR234 protein, with higher effect on Curli promoter 2010-10-23T11:00:00Z 2016-02-12T09:01:32Z pcr This part is coding for a mutated protein OmpR. This protein will be phosphorilated by the complex Envz. The phosphorilated protein is an activator of the Curli promoter false false _471_ 4206 6842 9 It's complicated true enter false Nathalie Isorce BBa_K540001 1 BBa_K540001 rcn, cobalt-sensitive promoter 2011-09-14T11:00:00Z 2015-07-14T12:34:23Z PCR from E.coli MC4100 genome. Released HQ 2013 Rcn is a promoter that is activated by cobalt. A repressor is fixed to the promoter by default, and cobalt binds to this repressor, which activates the gene under control of this promoter. Rcn is also activated by nickel, but we have not characterized this aspect. false false _706_ 4206 8419 9 In stock false An internal iGEM restriction site had to be mutated by QuickChange mutagenesis. false Mathilde Dumond annotation2129035 1 RcnR range2129035 1 1 279 BBa_K342003_sequence 1 aggaggtatataatgcaagagaactacaagattctggtggtcgatgacgacatgcgcctgcgtgcgctgctggaacgttatctcaccgaacaaggcttccaggttcgaagcgtcgctaatgcagaacagatggatcgccggctgactcgtgaatctttccatcttatggtactggatttaatgttacctggtgaagatggcttgtcgatttgccgacgtcttcgtagtcagagcaacccgatgccgatcattatggtgacggcgaaaggggaagaagtggaccgtatcgtaggcctggagattggcgctgacgactacattccaaaaccgtttaacccgcgtgaactgctggcccgtatccgtgcggtgctgcgtcgtcaggcgaacgaactgccaggcgcaccgtcacaggaagaggcggtaattgctttcggtaagttcaaacttaacctcggtacgcgcgaaatgttccgcgaagacgagccgatgccgctcaccagcggtgagtttgcggtactgaaggcactggtcagccatccgcgtgagccgctctcccgcgataagctgatgaaccttgcccgtggtcgtgaatattccgcaatggaacgctccatcgacgtgcagatttcgcgtctgcgccgcatggtggaagaagatccagcgcatccgcgttacattcagaccgtctggggtctgggctacgtctttgtaccggacggctctaaagcatgataataat BBa_K540001_sequence 1 ttattattatttgatatatgaatccagcaccttcagaacgacatccagatcttcttcacgttttagctcatccccctggtgaacgatgtgttccgtcagatgacctttaatcacttcccgcatcagaccgtttaccgcgccacggatagcagcaatctgttgtaaaactgctgcgcattcgtgcggctcgtcgagcattttcttgagcgccacgacctggccctgaatcttactggcacgcgctttcagtttctgtttatcacggattgtatgagacatggcaacacctggttaacaagaatatgaaaaatcatagcactattaatctactggggggtagtatcaggtactgggggggagtagaatcagattgccgaattaatactaagaattattatcatgaccgaatttacaactcttcttcag BBa_K540003_sequence 1 ttattattatttgatatatgaatccagcaccttcagaacgacatccagatcttcttcacgttttagctcatccccctggtgaacgatgtgttccgtcagatgacctttaatcacttcccgcatcagaccgtttaccgcgccacggatagcagcaatctgttgtaaaactgctgcgcattcgtgcggctcgtcgagcattttcttgagcgccacgacctggccctgaatcttactggcacgcgctttcagtttctgtttatcacggattgtatgagacatggcaacacctggttaacaagaatatgaaaaatcatagcactattaatctactggggggtagtatcaggtactgggggggagtagaatcagattgccgaattaatactaagaattattatcatgaccgaatttacaactcttcttcagtactagagaggaggtatataatgcaagagaactacaagattctggtggtcgatgacgacatgcgcctgcgtgcgctgctggaacgttatctcaccgaacaaggcttccaggttcgaagcgtcgctaatgcagaacagatggatcgccggctgactcgtgaatctttccatcttatggtactggatttaatgttacctggtgaagatggcttgtcgatttgccgacgtcttcgtagtcagagcaacccgatgccgatcattatggtgacggcgaaaggggaagaagtggaccgtatcgtaggcctggagattggcgctgacgactacattccaaaaccgtttaacccgcgtgaactgctggcccgtatccgtgcggtgctgcgtcgtcaggcgaacgaactgccaggcgcaccgtcacaggaagaggcggtaattgctttcggtaagttcaaacttaacctcggtacgcgcgaaatgttccgcgaagacgagccgatgccgctcaccagcggtgagtttgcggtactgaaggcactggtcagccatccgcgtgagccgctctcccgcgataagctgatgaaccttgcccgtggtcgtgaatattccgcaatggaacgctccatcgacgtgcagatttcgcgtctgcgccgcatggtggaagaagatccagcgcatccgcgttacattcagaccgtctggggtctgggctacgtctttgtaccggacggctctaaagcatgataataat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z