BBa_K541024 1 BBa_K541024 Reflectin 1A (SacB) and LALF (SacB) protein for B.Subtilis 2011-07-18T11:00:00Z 2015-05-08T01:12:38Z LALF from Horseshoe crab, Reflectin from Cephalopod, promoter-RBS from B.subtilis Reflectin is a self-assembling protein that has the ability to reflect the sun light which depends on the thickness of the protein layer. Therefore, we thought that this ability could be used as a novel reporter for B.subtilis and E.coli. On the other hand, Lipopolysaccharide (LPS), or endotoxin, is the major mediator of septic shock, a serious complication of Gram-negative bacterial infections in humans. Molecules that bind LPS and neutralize its biological effects or enhance its clearance could have important clinical applications. Limulus anti-LPS factor (LALF) binds LPS tightly, and, in animal models, reduces mortality when administered before or after LPS challenge or bacterial infection. The wedge- shaped molecule has a striking charge distribution and amphipathicity that suggest how it can insert into membranes. The binding site for LPS probably involves an extended amphipathic loop, and it has been proposed that two mammalian LPS-binding proteins will have a similar loop. The amphipathic loop structure may be used in the design of molecules with therapeutic properties against septic shock. false false _708_ 0 9573 9 Not in stock false Optimized for B.subtilis false Mustafa Elitok annotation2123581 1 BBa_K541020 range2123581 1 546 1523 annotation2123573 1 BBa_K541001 range2123573 1 1 537 annotation2123572 1 LALF range2123572 1 226 537 annotation2123574 1 BBa_K143012 range2123574 1 546 642 annotation2123578 1 rbs range2123578 1 651 662 annotation2123575 1 Sigma A-35 range2123575 1 608 613 annotation2123566 1 BBa_K143012 range2123566 1 1 97 annotation2123565 1 BBa_K143053 range2123565 1 1 117 annotation2123569 1 BBa_K143021 range2123569 1 106 117 annotation2123576 1 Sigma A -10 range2123576 1 631 636 annotation2123577 1 BBa_K143021 range2123577 1 651 662 annotation2123579 1 Reflectin range2123579 1 669 1523 annotation2123571 1 SacB range2123571 1 124 219 annotation2123568 1 Sigma A -10 range2123568 1 86 91 annotation2123570 1 rbs range2123570 1 106 117 annotation2123567 1 Sigma A-35 range2123567 1 63 68 annotation2123580 1 BBa_K541013 range2123580 1 669 1523 BBa_K541024_sequence 1 aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatactagatgaacatcaaaaaatttgcaaaacaggcgacagttctgacatttacaacagcactgcttgcaggcggcgcaacacaagcatttgcaaaagaaacatactagatggatggaatttggacacaactgatttttaccctggtgaagaaccttgctacactttggcaatccggcgattttcagttccttgaccatgaatgccattaccgtattaaacctacgtttagacgcttgaagtggaagtataaaggaaaattttggtgcccgtcttggaccagcattactggacgtgcgacaaagtcatcacgctccggtgccgtcgaacattcggtccgaaattttgttgggcaagcgaagtcgtcaggcctgataacgcaaagacaagcggaacagtttattagtcaatacaattaataatactagagaattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatactagatgaatcgctttatgaatcgctatcgcccgatgttcaataatatgtactctaacatgtaccgtggccgctaccgcggtatgatggaaccgatgagccgcatgactatggactttcagggccgctacatggacagccaaggccgcatggtggacccgcgctactacgattactacggccgcttcaacgattacgaccgctactatggccgcagcatgttcaactatggctggatgatggacggcgatcgctacaaccgctataaccgctggatggattacccggagcgctacatggatatgtctggctaccagatggatatgtctggccgctggatggatatgcagggccgccactgcaacccgtattctcagtggatgatgtacaactacaaccgccatggctactacccgaactactcttacggccgccacatgttttatccggaacgctggatggacatgtctaactactctatggacatgtatggccgctatatggatcgctggggccgctactgtaacccgttttctcagtacatgaactactacggccgctactggaactacccgggctacaataattactactattctcgcaacatgtattacccggaacgctactttgacatgtctaactggcagatggacatgcaaggccgctggatggacaaccagggccgctattgttccccgtactggaacaactggtacggccgccatatgtactacccgtaccagaacaactacttctacggccgctatgactacccgggcatggactattccaactaccaaatggacatgcagggccgctacatggaccagtacggcatgaacgactactactattaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z