BBa_K541024
1
BBa_K541024
Reflectin 1A (SacB) and LALF (SacB) protein for B.Subtilis
2011-07-18T11:00:00Z
2015-05-08T01:12:38Z
LALF from Horseshoe crab, Reflectin from Cephalopod, promoter-RBS from B.subtilis
Reflectin is a self-assembling protein that has the ability to reflect the sun light which depends on the thickness of the protein layer. Therefore, we thought that this ability could be used as a novel reporter for B.subtilis and E.coli.
On the other hand, Lipopolysaccharide (LPS), or endotoxin, is the major mediator of septic shock, a serious complication of Gram-negative bacterial infections in humans. Molecules that bind LPS and neutralize its biological effects or enhance its clearance could have important clinical applications. Limulus anti-LPS factor (LALF) binds LPS tightly, and, in animal models, reduces mortality when administered before or after LPS challenge or bacterial infection. The wedge- shaped molecule has a striking charge distribution and amphipathicity that suggest how it can insert into membranes. The binding site for LPS probably involves an extended amphipathic loop, and it has been proposed that two mammalian LPS-binding proteins will have a similar loop. The amphipathic loop structure may be used in the design of molecules with therapeutic properties against septic shock.
false
false
_708_
0
9573
9
Not in stock
false
Optimized for B.subtilis
false
Mustafa Elitok
annotation2123581
1
BBa_K541020
range2123581
1
546
1523
annotation2123573
1
BBa_K541001
range2123573
1
1
537
annotation2123572
1
LALF
range2123572
1
226
537
annotation2123574
1
BBa_K143012
range2123574
1
546
642
annotation2123578
1
rbs
range2123578
1
651
662
annotation2123575
1
Sigma A-35
range2123575
1
608
613
annotation2123566
1
BBa_K143012
range2123566
1
1
97
annotation2123565
1
BBa_K143053
range2123565
1
1
117
annotation2123569
1
BBa_K143021
range2123569
1
106
117
annotation2123576
1
Sigma A -10
range2123576
1
631
636
annotation2123577
1
BBa_K143021
range2123577
1
651
662
annotation2123579
1
Reflectin
range2123579
1
669
1523
annotation2123571
1
SacB
range2123571
1
124
219
annotation2123568
1
Sigma A -10
range2123568
1
86
91
annotation2123570
1
rbs
range2123570
1
106
117
annotation2123567
1
Sigma A-35
range2123567
1
63
68
annotation2123580
1
BBa_K541013
range2123580
1
669
1523
BBa_K541024_sequence
1
aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatactagatgaacatcaaaaaatttgcaaaacaggcgacagttctgacatttacaacagcactgcttgcaggcggcgcaacacaagcatttgcaaaagaaacatactagatggatggaatttggacacaactgatttttaccctggtgaagaaccttgctacactttggcaatccggcgattttcagttccttgaccatgaatgccattaccgtattaaacctacgtttagacgcttgaagtggaagtataaaggaaaattttggtgcccgtcttggaccagcattactggacgtgcgacaaagtcatcacgctccggtgccgtcgaacattcggtccgaaattttgttgggcaagcgaagtcgtcaggcctgataacgcaaagacaagcggaacagtttattagtcaatacaattaataatactagagaattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatactagatgaatcgctttatgaatcgctatcgcccgatgttcaataatatgtactctaacatgtaccgtggccgctaccgcggtatgatggaaccgatgagccgcatgactatggactttcagggccgctacatggacagccaaggccgcatggtggacccgcgctactacgattactacggccgcttcaacgattacgaccgctactatggccgcagcatgttcaactatggctggatgatggacggcgatcgctacaaccgctataaccgctggatggattacccggagcgctacatggatatgtctggctaccagatggatatgtctggccgctggatggatatgcagggccgccactgcaacccgtattctcagtggatgatgtacaactacaaccgccatggctactacccgaactactcttacggccgccacatgttttatccggaacgctggatggacatgtctaactactctatggacatgtatggccgctatatggatcgctggggccgctactgtaacccgttttctcagtacatgaactactacggccgctactggaactacccgggctacaataattactactattctcgcaacatgtattacccggaacgctactttgacatgtctaactggcagatggacatgcaaggccgctggatggacaaccagggccgctattgttccccgtactggaacaactggtacggccgccatatgtactacccgtaccagaacaactacttctacggccgctatgactacccgggcatggactattccaactaccaaatggacatgcagggccgctacatggaccagtacggcatgaacgactactactattaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z