BBa_K143012 1 Pveg Promoter veg a constitutive promoter for B. subtilis 2008-09-10T11:00:00Z 2015-05-08T01:10:23Z The Pveg promoter was suggested to us by Dr. Jan-Willem Veening of Newcastle University. This sequence supplied was compared to that of the DBTBS database<cite>#3</cite> then a section containing the binding site synthesised by Geneart. Released HQ 2013 Pveg is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. Pveg contains binding sites for the ''B.sutbilis'' major sigma factor<cite>#1</cite>. Pveg in ''B.subtilis'' utilises two binding sites to cause high expression of genes<cite>#2</cite>, however our Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulatoion factor spoOA interacts with Pveg though it is not known how<cite>#3</cite>. The context with which we used the promoter Pveg is as a '''Polymerase Per Second''' (PoPS) generator. false true _199_ 0 2090 9 In stock false The biobrick part was designed to include a single binding site for the ''B.subtilis major sigma factor. In addition the biobrick standard was applied to the promoter Pveg sequence. false James Chappell annotation1975704 1 Sigma A-35 range1975704 1 63 68 annotation1975705 1 Sigma A -10 range1975705 1 86 91 BBa_K541026 1 BBa_K541026 Reflectin 1A (SacB) and LALF (LipA) protein for B.subtilis 2011-07-18T11:00:00Z 2015-05-08T01:12:38Z LALF from horseshoe crab, Reflectin from Cephalopod, promoter-RBS from B.subtilis Reflectin is a self-assembling protein that has the ability to reflect the sun light which depends on the thickness of the protein layer. Therefore, we thought that this ability could be used as a novel reporter for B.subtilis and E.coli. On the other hand, Lipopolysaccharide (LPS), or endotoxin, is the major mediator of septic shock, a serious complication of Gram-negative bacterial infections in humans. Molecules that bind LPS and neutralize its biological effects or enhance its clearance could have important clinical applications. Limulus anti-LPS factor (LALF) binds LPS tightly, and, in animal models, reduces mortality when administered before or after LPS challenge or bacterial infection. The wedge- shaped molecule has a striking charge distribution and amphipathicity that suggest how it can insert into membranes. The binding site for LPS probably involves an extended amphipathic loop, and it has been proposed that two mammalian LPS-binding proteins will have a similar loop. The amphipathic loop structure may be used in the design of molecules with therapeutic properties against septic shock. false false _708_ 0 9573 9 Not in stock false Optimized for B.subtilis false Mustafa Elitok component2123614 1 BBa_K143021 component2123609 1 BBa_K143012 component2123601 1 BBa_K143012 component2123604 1 BBa_K143021 annotation2123601 1 BBa_K143012 range2123601 1 1 97 annotation2123609 1 BBa_K143012 range2123609 1 987 1083 annotation2123604 1 BBa_K143021 range2123604 1 106 117 annotation2123614 1 BBa_K143021 range2123614 1 1092 1103 BBa_K143021 1 RBS-spoVG SpoVG ribosome binding site (RBS) for B. subtilis 2008-09-16T11:00:00Z 2015-05-08T01:10:23Z The sequence was taken from a previous research paper [1] and was constructed by Geneart. Released HQ 2013 Description: SpoVG is an endogenous ribosome binding site from B.subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B.subtilis. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -19kcal <cite>1</cite> false true _199_ 0 2090 9 In stock false In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained. false James Chappell annotation1975997 1 rbs range1975997 1 1 12 BBa_K143021_sequence 1 aaaggtggtgaa BBa_K143012_sequence 1 aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgt BBa_K541026_sequence 1 aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatactagatgaatcgctttatgaatcgctatcgcccgatgttcaataatatgtactctaacatgtaccgtggccgctaccgcggtatgatggaaccgatgagccgcatgactatggactttcagggccgctacatggacagccaaggccgcatggtggacccgcgctactacgattactacggccgcttcaacgattacgaccgctactatggccgcagcatgttcaactatggctggatgatggacggcgatcgctacaaccgctataaccgctggatggattacccggagcgctacatggatatgtctggctaccagatggatatgtctggccgctggatggatatgcagggccgccactgcaacccgtattctcagtggatgatgtacaactacaaccgccatggctactacccgaactactcttacggccgccacatgttttatccggaacgctggatggacatgtctaactactctatggacatgtatggccgctatatggatcgctggggccgctactgtaacccgttttctcagtacatgaactactacggccgctactggaactacccgggctacaataattactactattctcgcaacatgtattacccggaacgctactttgacatgtctaactggcagatggacatgcaaggccgctggatggacaaccagggccgctattgttccccgtactggaacaactggtacggccgccatatgtactacccgtaccagaacaactacttctacggccgctatgactacccgggcatggactattccaactaccaaatggacatgcagggccgctacatggaccagtacggcatgaacgactactactattaataatactagagaattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatactagatgaaatttgtgaaaagacgcattattgcactggttacaattctgatgctgtcagttacatcactgtttgcactgcaaccgtcagcaaaagcagcagaacattactagatggatggaatttggacacaactgatttttaccctggtgaagaaccttgctacactttggcaatccggcgattttcagttccttgaccatgaatgccattaccgtattaaacctacgtttagacgcttgaagtggaagtataaaggaaaattttggtgcccgtcttggaccagcattactggacgtgcgacaaagtcatcacgctccggtgccgtcgaacattcggtccgaaattttgttgggcaagcgaagtcgtcaggcctgataacgcaaagacaagcggaacagtttattagtcaatacaattaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z