BBa_K541515
1
BBa_K541515
Limulus anti-LPS factor (LALF) for Bacillus subtilis
2011-07-12T11:00:00Z
2015-05-08T01:12:38Z
Horseshoe crabs (Limulus polyphemus and Tachypleus tridentatus) are ancient arachnids that possess a primitive circulatory
system, the hemolymph, containing only one kind of cell, the hemocyte. Exposure of hemocytes to bacterial endotoxins [lipopolysaccharide (LPS)] results in the activation of an intracellular coagulation cascade (Iwanaga et al., 1986), a defense against microbial invasion
Constitutive promoter veg(BBa_K143012) coupled to the strong Ribosome Binding Site spoVG(BBa_K143021) from B. subtilis. These parts have
been taken from 2008_Imperial_Collage. Pveg is a constitutive promoter that constitutively expresses the P43 protein in B. subtilis.
SpoVG is an endogenous ribosome binding site from B. subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is
complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B. subtilis.
SacB is a signal peptide used in the Sec-SRP (secretory signal recognition particle) pathway by B. subtilis. Signal peptides are
responsible for directing preproteins (secretory proteins with a signal peptide region attached) through an appropriate secretory
pathway. In the case of the Sec-SRP signal peptide, they direct preproteins from the cytoplasm into the growth medium.
Lipopolysaccharide (LPS), or endotoxin, is the major mediator of septic shock, a serious complication of Gram-negative bacterial
infections in humans. Molecules that bind LPS and neutralize its biological effects or enhance its clearance could have important
clinical applications. Limulus anti-LPS factor (LALF) binds LPS
tightly, and, in animal models, reduces mortality when administered before or after LPS challenge or bacterial infection. The wedge-
shaped molecule has a striking charge distribution and amphipathicity that suggest how it can insert into membranes. The binding site
for LPS probably involves an extended amphipathic loop, and it has been proposed that two mammalian LPS-binding proteins will have a
similar loop. The amphipathic loop structure may be used in the design of molecules with therapeutic properties against septic shock.
false
false
_708_
0
4260
9
Not in stock
true
During our design, we have looked out that this anti-LPS factor gene should be transfered into gram positive bacteria (i.e. B.subtilis) because of absence of LPS in gram positive bacteria.
false
Cihan TAŞTAN
annotation2123335
1
Sigma A-35
range2123335
1
63
68
annotation2123333
1
BBa_K143012
range2123333
1
1
97
annotation2123338
1
BBa_K143021
range2123338
1
106
117
annotation2123334
1
BBa_K143053
range2123334
1
1
117
annotation2123336
1
Sigma A -10
range2123336
1
86
91
annotation2123339
1
SacB
range2123339
1
124
219
annotation2123337
1
rbs
range2123337
1
106
117
annotation2123340
1
LALF
range2123340
1
226
537
BBa_K541515_sequence
1
aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatactagatgaacatcaaaaaatttgcaaaacaggcgacagttctgacatttacaacagcactgcttgcaggcggcgcaacacaagcatttgcaaaagaaacatactagatggatggaatttggacacaactgatttttaccctggtgaagaaccttgctacactttggcaatccggcgattttcagttccttgaccatgaatgccattaccgtattaaacctacgtttagacgcttgaagtggaagtataaaggaaaattttggtgcccgtcttggaccagcattactggacgtgcgacaaagtcatcacgctccggtgccgtcgaacattcggtccgaaattttgttgggcaagcgaagtcgtcaggcctgataacgcaaagacaagcggaacagtttattagtcaatacaattaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z