LOCUS       BBa_K541546             1310 bp    DNA     linear       01-Jun-2024
DEFINITION  IPTG inducible PlacI, RBS,TAT signal peptide and reflectin
ACCESSION   BBa_K541546
VERSION     BBa_K541546.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     misc_feature    209..220
                     /label=BBa_B0034
     misc_feature    213..216
                     /label=conserved
     misc_feature    227..455
                     /label=TAT signalling peptide
     misc_feature    1..455
                     /label=BBa_K541016
     CDS             462..1310
                     /label=Reflectin
     misc_feature    462..1310
                     /label=BBa_K541013
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagcaat acgcaaaccg
      241 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
      301 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
      361 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
      421 cacacatact agagattaaa gaggagaaat actagatgaa tcgctttatg aatcgctatc
      481 gcccgatgtt caataatatg tactctaaca tgtaccgtgg ccgctaccgc ggtatgatgg
      541 aaccgatgag ccgcatgact atggactttc agggccgcta catggacagc caaggccgca
      601 tggtggaccc gcgctactac gattactacg gccgcttcaa cgattacgac cgctactatg
      661 gccgcagcat gttcaactat ggctggatga tggacggcga tcgctacaac cgctataacc
      721 gctggatgga ttacccggag cgctacatgg atatgtctgg ctaccagatg gatatgtctg
      781 gccgctggat ggatatgcag ggccgccact gcaacccgta ttctcagtgg atgatgtaca
      841 actacaaccg ccatggctac tacccgaact actcttacgg ccgccacatg ttttatccgg
      901 aacgctggat ggacatgtct aactactcta tggacatgta tggccgctat atggatcgct
      961 ggggccgcta ctgtaacccg ttttctcagt acatgaacta ctacggccgc tactggaact
     1021 acccgggcta caataattac tactattctc gcaacatgta ttacccggaa cgctactttg
     1081 acatgtctaa ctggcagatg gacatgcaag gccgctggat ggacaaccag ggccgctatt
     1141 gttccccgta ctggaacaac tggtacggcc gccatatgta ctacccgtac cagaacaact
     1201 acttctacgg ccgctatgac tacccgggca tggactattc caactaccaa atggacatgc
     1261 agggccgcta catggaccag tacggcatga acgactacta ctattaataa
//