LOCUS BBa_K541800 6285 bp DNA linear 29-Nov-2024 DEFINITION B.subtilis and E.coli Episomal Shuttle Vector with Consitutive RFP to express in E.coli ACCESSION BBa_K541800 VERSION BBa_K541800.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Biobrick Suffix misc_feature 5187..5208 /label=Biobrick Prefix misc_feature 4120..4981 /label=Beta-Lactamase misc_feature 2176..2924 /label=Chloramphenicol Acetyl-Transferase misc_feature 723..1742 /label=Gram Positive Replication Protein misc_feature 399..649 /label=puc9 Plasmid (E. coli rep origin) misc_feature 1..5208 /label=BBa_K090401 CDS 5217..5304 /label=end of LacI coding region (inactive) misc_feature 5217..5416 /label=BBa_R0010 misc_feature 5305..5342 /label=CAP binding site promoter 5353..5358 /label=-35 promoter 5377..5382 /label=-10 misc_feature 5382..5416 /label=LacI binding site misc_feature 5389..5389 /label=start promoter 5217..5416 /label=BBa_R0010 misc_feature 5429..5432 /label=conserved RBS 5425..5436 /label=BBa_B0034 CDS 5443..6117 /label=mrfp1 misc_feature 6124..6148 /label=Help:Barcodes CDS 5443..6123 /label=BBa_E1010 stem_loop 6168..6211 /label=stem_loop misc_feature 6157..6236 /label=BBa_B0010 terminator 6157..6236 /label=BBa_B0010 stem_loop 6252..6271 /label=T7 TE misc_feature 6278..6278 /label=stop polyA_site 6272..6285 /label=polya misc_feature 6245..6285 /label=BBa_B0012 terminator 6245..6285 /label=BBa_B0012 misc_feature 5217..6285 /label=BBa_J04450 ORIGIN 1 tactagtagc ggccgctgca gaagcttgag taggacaaat ccgccgagct tcgacgagat 61 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata 121 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct 181 ataaccagac cgttcagaac aaagaataca agaaaatatt tacaaaaaat caatttaaca 241 attccttaaa acatgcagga attgacgatt taaacaatat tagctttgaa caattcttat 301 ctcttttcaa tagctataaa ttatttaata agtaagttaa gggatgcata aactgcatcc 361 cttaacttgt ttttcgtgtg cctatttttt gtgaatcgct aagaaaccat tattatcatg 421 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat 481 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg 541 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc 601 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatac aaaacatatt 661 tcaacacaat acaaatgggt tagttaaaaa agcaggcctt ctaaaggtct gcttttttta 721 tttgattatg taatttttaa tgccaggatg ccaataagcc ataacctcaa atgcaccatt 781 tgcaacctcg tcatcttctt cctcaatctt gaccagatcg ccgtcctccg catcaccaag 841 attcagctct ttatgtatct ccttcaaaat gccaccgtat ccaattaacc ttcgagctgc 901 caacgcatca tccaagtaaa gcaccgtgtt cagattgtct tcagtcacct tattaccgcg 961 cacacaatcc gtatccttaa ccggatattt agagatttcg cgaacagctt tttgctccat 1021 cattgcgttc cgcacatcgt tttcaatctg ttcagcgtca atcttagctt tacctttcac 1081 tcgacgaata tcgacaattg gagtgtaatc caatttcatc gcctttttcc aaaggctcgt 1141 ccactccgcc tgcttaatat agtttttccc aaaataattt ttccttactg gtatcaacac 1201 atgaaaatga ggatgatatg tatcttcttc atgatttttg gtaatctcta aagctctgaa 1261 aaatccaaga accgaagttt ttactttttt gtactggaac agtttcctaa agccttccat 1321 catcgcagaa atttgtggct tcagccgttc tccctttaca tttcgaatcg tcagcgtgag 1381 aaaaatccat ccgcagccgt actgtctatt ggcttcctct acgatcaact tattgtgata 1441 agcaattttt aacgacctgc gccacgcaca catcggacat aacctcactt tacaaaaatg 1501 ggcttgatac agttttaact tgcccgtctc cgggtctctc ttaaacgaaa gatactctgc 1561 acaactaatt agtttttcag cctttttgcc atagtaaggt gccccaatct tactctctaa 1621 cgcttcgtaa tgctccgcca tgaggttcgt ccgtctcttt ttccccttcc aatcccgctt 1681 tttacctgtt gcggttttat cttcgaggat gctataatca ttttcagatg aataaatcaa 1741 caaaaaaact ccttctgagc tagttctcta gcattctatt attttgattc gacaccttaa 1801 taatagcaga aggagttttt acctgtcaaa gaaccatcaa acccttgata cacaaggctt 1861 tgacctaatt ttgaaaaatg atgttgtttc tatatagtat caagataaga aagaaaagga 1921 tttttcgcta cgctcaaatc ctttaaaaaa acacaaaaga ccacattttt taatgtggtc 1981 tttattcttc aactaaagca cccattagtt caacaaacga aaattggata aagtgggata 2041 tttttaaaat atatatttat gttacagtaa tattgacttt taaaaaagga ttgattctaa 2101 tgaagaaagc agacaagtaa gcctcctaaa ttcactttag ataaaaattt aggaggcata 2161 tcaaatgaac tttaataaaa ttgatttaga caattggaag agaaaagaga tatttaatca 2221 ttatttgaac caacaaacga cttttagtat aaccacagaa attgatatta gtgttttata 2281 ccgaaacata aaacaagaag gatataaatt ttaccctgca tttattttct tagtgacaag 2341 ggtgataaac tcaaatacag cttttagaac tggttacaat agcgacggag agttaggtta 2401 ttgggataag ttagagccac tttatacaat ttttgatggt gtatctaaaa cattctctgg 2461 tatttggact cctgtaaaga atgacttcaa agagttttat gatttatacc tttctgatgt 2521 agagaaatat aatggttcgg ggaaattgtt tcccaaaaca cctatacctg aaaatgcttt 2581 ttctctttct attattccat ggacttcatt tactgggttt aacttaaata tcaataataa 2641 tagtaattac cttctaccca ttattacagc aggaaaattc attaataaag gtaattcaat 2701 atatttaccg ctatctttac aggtacatca ttctgtttgt gatggttatc atgcaggatt 2761 gtttatgaac tctattcagg aattgtcaga taggcctaat gactggcttt tataatatga 2821 gataatgccg actgtacttt ttacagtcgg ttttctaatg tcactaacct gccccgttag 2881 tcgccattcg ccagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 2941 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt 3001 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc 3061 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 3121 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct 3181 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 3241 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 3301 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 3361 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 3421 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 3481 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 3541 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 3601 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 3661 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 3721 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 3781 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 3841 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 3901 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 3961 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 4021 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 4081 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 4141 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 4201 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 4261 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 4321 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 4381 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgccgcag 4441 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 4501 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 4561 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 4621 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 4681 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac 4741 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 4801 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 4861 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 4921 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 4981 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 5041 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 5101 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 5161 gtatcacgag gccctttcgt cttcaagaat tcgcggccgc ttctagagta ctagagcaat 5221 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 5281 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 5341 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 5401 ataacaattt cacacatact agagaaagag gagaaatact agatggcttc ctccgaagac 5461 gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag 5521 ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg 5581 aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag 5641 tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc 5701 ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc 5761 gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt 5821 accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc 5881 accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa 5941 ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg 6001 gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa 6061 gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa 6121 taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg 6181 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 6241 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //