##gff-version 3
##sequence-region BBa_K541800 1 6285
BBa_K541800	.	engineered_region	5217	6285	.	+	0	ID=BBa_J04450;Name=BBa_J04450
BBa_K541800	.	ribosome_entry_site	5425	5436	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K541800	.	conserved_region	5429	5432	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K541800	.	CDS	5443	6123	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K541800	.	engineered_tag	6124	6148	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K541800	.	CDS	5443	6117	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K541800	.	terminator	6157	6236	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K541800	.	stem_loop	6168	6211	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K541800	.	engineered_region	6157	6236	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K541800	.	promoter	5217	5416	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K541800	.	promoter	5353	5358	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K541800	.	engineered_region	5217	5416	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K541800	.	start_codon	5389	5389	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K541800	.	CDS	5217	5304	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K541800	.	non_covalent_binding_site	5305	5342	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K541800	.	promoter	5377	5382	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K541800	.	non_covalent_binding_site	5382	5416	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K541800	.	terminator	6245	6285	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K541800	.	polyA_site	6272	6285	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K541800	.	stop_codon	6278	6278	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K541800	.	stem_loop	6252	6271	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K541800	.	engineered_region	6245	6285	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K541800	.	plasmid	1	5208	.	+	0	ID=BBa_K090401;Name=BBa_K090401
BBa_K541800	.	sequence_feature	4120	4981	.	+	0	ID=annotation1992771;Name=Beta-Lactamase;Parent=BBa_K090401
BBa_K541800	.	sequence_feature	2176	2924	.	+	0	ID=annotation1992772;Name=Chloramphenicol Acetyl-Transferase;Parent=BBa_K090401
BBa_K541800	.	sequence_feature	399	649	.	+	0	ID=annotation1992774;Name=puc9 Plasmid (E. coli rep origin);Parent=BBa_K090401
BBa_K541800	.	sequence_feature	723	1742	.	+	0	ID=annotation1992773;Name=Gram Positive Replication Protein;Parent=BBa_K090401
BBa_K541800	.	engineered_region	1	21	.	+	0	ID=annotation1992769;Name=Biobrick Suffix;Parent=BBa_K090401
BBa_K541800	.	engineered_region	5187	5208	.	+	0	ID=annotation1992770;Name=Biobrick Prefix;Parent=BBa_K090401
>BBa_K541800
tactagtagcggccgctgcagaagcttgagtaggacaaatccgccgagcttcgacgagattttcaggagctaaggaagc
taaaatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcattt
cagtcagttgctcaatgtacctataaccagaccgttcagaacaaagaatacaagaaaatatttacaaaaaatcaattta
acaattccttaaaacatgcaggaattgacgatttaaacaatattagctttgaacaattcttatctcttttcaatagcta
taaattatttaataagtaagttaagggatgcataaactgcatcccttaacttgtttttcgtgtgcctattttttgtgaa
tcgctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtctcgcgcgtttc
ggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagca
gacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgt
actgagagtgcaccatacaaaacatatttcaacacaatacaaatgggttagttaaaaaagcaggccttctaaaggtctg
ctttttttatttgattatgtaatttttaatgccaggatgccaataagccataacctcaaatgcaccatttgcaacctcg
tcatcttcttcctcaatcttgaccagatcgccgtcctccgcatcaccaagattcagctctttatgtatctccttcaaaa
tgccaccgtatccaattaaccttcgagctgccaacgcatcatccaagtaaagcaccgtgttcagattgtcttcagtcac
cttattaccgcgcacacaatccgtatccttaaccggatatttagagatttcgcgaacagctttttgctccatcattgcg
ttccgcacatcgttttcaatctgttcagcgtcaatcttagctttacctttcactcgacgaatatcgacaattggagtgt
aatccaatttcatcgcctttttccaaaggctcgtccactccgcctgcttaatatagtttttcccaaaataatttttcct
tactggtatcaacacatgaaaatgaggatgatatgtatcttcttcatgatttttggtaatctctaaagctctgaaaaat
ccaagaaccgaagtttttacttttttgtactggaacagtttcctaaagccttccatcatcgcagaaatttgtggcttca
gccgttctccctttacatttcgaatcgtcagcgtgagaaaaatccatccgcagccgtactgtctattggcttcctctac
gatcaacttattgtgataagcaatttttaacgacctgcgccacgcacacatcggacataacctcactttacaaaaatgg
gcttgatacagttttaacttgcccgtctccgggtctctcttaaacgaaagatactctgcacaactaattagtttttcag
cctttttgccatagtaaggtgccccaatcttactctctaacgcttcgtaatgctccgccatgaggttcgtccgtctctt
tttccccttccaatcccgctttttacctgttgcggttttatcttcgaggatgctataatcattttcagatgaataaatc
aacaaaaaaactccttctgagctagttctctagcattctattattttgattcgacaccttaataatagcagaaggagtt
tttacctgtcaaagaaccatcaaacccttgatacacaaggctttgacctaattttgaaaaatgatgttgtttctatata
gtatcaagataagaaagaaaaggatttttcgctacgctcaaatcctttaaaaaaacacaaaagaccacattttttaatg
tggtctttattcttcaactaaagcacccattagttcaacaaacgaaaattggataaagtgggatatttttaaaatatat
atttatgttacagtaatattgacttttaaaaaaggattgattctaatgaagaaagcagacaagtaagcctcctaaattc
actttagataaaaatttaggaggcatatcaaatgaactttaataaaattgatttagacaattggaagagaaaagagata
tttaatcattatttgaaccaacaaacgacttttagtataaccacagaaattgatattagtgttttataccgaaacataa
aacaagaaggatataaattttaccctgcatttattttcttagtgacaagggtgataaactcaaatacagcttttagaac
tggttacaatagcgacggagagttaggttattgggataagttagagccactttatacaatttttgatggtgtatctaaa
acattctctggtatttggactcctgtaaagaatgacttcaaagagttttatgatttatacctttctgatgtagagaaat
ataatggttcggggaaattgtttcccaaaacacctatacctgaaaatgctttttctctttctattattccatggacttc
atttactgggtttaacttaaatatcaataataatagtaattaccttctacccattattacagcaggaaaattcattaat
aaaggtaattcaatatatttaccgctatctttacaggtacatcattctgtttgtgatggttatcatgcaggattgttta
tgaactctattcaggaattgtcagataggcctaatgactggcttttataatatgagataatgccgactgtactttttac
agtcggttttctaatgtcactaacctgccccgttagtcgccattcgccagctgcctcgcgcgtttcggtgatgacggtg
aaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtca
gggcgcgtcagcgggtgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtgtatactggc
ttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggag
aaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggta
tcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggcc
agcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaa
aaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctc
gtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctc
aatgctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttca
gcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagca
gccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggct
acactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatc
cggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaa
gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagat
tatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaac
ttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcc
tgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacc
cacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaacttt
atccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgtt
gttgccattgccgcaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaa
ggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagtt
ggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttct
gtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaacac
gggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaag
gatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcacc
agcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatac
tcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtat
ttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatc
atgacattaacctataaaaataggcgtatcacgaggccctttcgtcttcaagaattcgcggccgcttctagagtactag
agcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaa
gcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccgg
ctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcc
tccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcg
aaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgtt
cgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactac
ctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttaccc
aggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggt
tatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatc
aaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttc
agctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagta
cgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttata