##gff-version 3
##sequence-region BBa_K541815 1 5753
BBa_K541815	.	plasmid	1	5208	.	+	0	ID=BBa_K090401;Name=BBa_K090401
BBa_K541815	.	sequence_feature	4120	4981	.	+	0	ID=annotation1992771;Name=Beta-Lactamase;Parent=BBa_K090401
BBa_K541815	.	sequence_feature	2176	2924	.	+	0	ID=annotation1992772;Name=Chloramphenicol Acetyl-Transferase;Parent=BBa_K090401
BBa_K541815	.	sequence_feature	399	649	.	+	0	ID=annotation1992774;Name=puc9 Plasmid (E. coli rep origin);Parent=BBa_K090401
BBa_K541815	.	sequence_feature	723	1742	.	+	0	ID=annotation1992773;Name=Gram Positive Replication Protein;Parent=BBa_K090401
BBa_K541815	.	engineered_region	1	21	.	+	0	ID=annotation1992769;Name=Biobrick Suffix;Parent=BBa_K090401
BBa_K541815	.	engineered_region	5187	5208	.	+	0	ID=annotation1992770;Name=Biobrick Prefix;Parent=BBa_K090401
BBa_K541815	.	engineered_region	5217	5753	.	+	0	ID=BBa_K541515;Name=BBa_K541515
BBa_K541815	.	ribosome_entry_site	5322	5333	.	+	0	ID=annotation2123337;Name=rbs;Parent=BBa_K541515
BBa_K541815	.	CDS	5442	5753	.	+	0	ID=annotation2123340;Name=LALF;Parent=BBa_K541515
BBa_K541815	.	engineered_region	5322	5333	.	+	0	ID=annotation2123338;Name=BBa_K143021;Parent=BBa_K541515
BBa_K541815	.	promoter	5279	5284	.	+	0	ID=annotation2123335;Name=Sigma A-35;Parent=BBa_K541515
BBa_K541815	.	promoter	5302	5307	.	+	0	ID=annotation2123336;Name=Sigma A -10;Parent=BBa_K541515
BBa_K541815	.	engineered_region	5217	5333	.	+	0	ID=annotation2123334;Name=BBa_K143053;Parent=BBa_K541515
BBa_K541815	.	engineered_region	5217	5313	.	+	0	ID=annotation2123333;Name=BBa_K143012;Parent=BBa_K541515
BBa_K541815	.	tag	5340	5435	.	+	0	ID=annotation2123339;Name=SacB;Parent=BBa_K541515
>BBa_K541815
tactagtagcggccgctgcagaagcttgagtaggacaaatccgccgagcttcgacgagattttcaggagctaaggaagc
taaaatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcattt
cagtcagttgctcaatgtacctataaccagaccgttcagaacaaagaatacaagaaaatatttacaaaaaatcaattta
acaattccttaaaacatgcaggaattgacgatttaaacaatattagctttgaacaattcttatctcttttcaatagcta
taaattatttaataagtaagttaagggatgcataaactgcatcccttaacttgtttttcgtgtgcctattttttgtgaa
tcgctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtctcgcgcgtttc
ggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagca
gacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgt
actgagagtgcaccatacaaaacatatttcaacacaatacaaatgggttagttaaaaaagcaggccttctaaaggtctg
ctttttttatttgattatgtaatttttaatgccaggatgccaataagccataacctcaaatgcaccatttgcaacctcg
tcatcttcttcctcaatcttgaccagatcgccgtcctccgcatcaccaagattcagctctttatgtatctccttcaaaa
tgccaccgtatccaattaaccttcgagctgccaacgcatcatccaagtaaagcaccgtgttcagattgtcttcagtcac
cttattaccgcgcacacaatccgtatccttaaccggatatttagagatttcgcgaacagctttttgctccatcattgcg
ttccgcacatcgttttcaatctgttcagcgtcaatcttagctttacctttcactcgacgaatatcgacaattggagtgt
aatccaatttcatcgcctttttccaaaggctcgtccactccgcctgcttaatatagtttttcccaaaataatttttcct
tactggtatcaacacatgaaaatgaggatgatatgtatcttcttcatgatttttggtaatctctaaagctctgaaaaat
ccaagaaccgaagtttttacttttttgtactggaacagtttcctaaagccttccatcatcgcagaaatttgtggcttca
gccgttctccctttacatttcgaatcgtcagcgtgagaaaaatccatccgcagccgtactgtctattggcttcctctac
gatcaacttattgtgataagcaatttttaacgacctgcgccacgcacacatcggacataacctcactttacaaaaatgg
gcttgatacagttttaacttgcccgtctccgggtctctcttaaacgaaagatactctgcacaactaattagtttttcag
cctttttgccatagtaaggtgccccaatcttactctctaacgcttcgtaatgctccgccatgaggttcgtccgtctctt
tttccccttccaatcccgctttttacctgttgcggttttatcttcgaggatgctataatcattttcagatgaataaatc
aacaaaaaaactccttctgagctagttctctagcattctattattttgattcgacaccttaataatagcagaaggagtt
tttacctgtcaaagaaccatcaaacccttgatacacaaggctttgacctaattttgaaaaatgatgttgtttctatata
gtatcaagataagaaagaaaaggatttttcgctacgctcaaatcctttaaaaaaacacaaaagaccacattttttaatg
tggtctttattcttcaactaaagcacccattagttcaacaaacgaaaattggataaagtgggatatttttaaaatatat
atttatgttacagtaatattgacttttaaaaaaggattgattctaatgaagaaagcagacaagtaagcctcctaaattc
actttagataaaaatttaggaggcatatcaaatgaactttaataaaattgatttagacaattggaagagaaaagagata
tttaatcattatttgaaccaacaaacgacttttagtataaccacagaaattgatattagtgttttataccgaaacataa
aacaagaaggatataaattttaccctgcatttattttcttagtgacaagggtgataaactcaaatacagcttttagaac
tggttacaatagcgacggagagttaggttattgggataagttagagccactttatacaatttttgatggtgtatctaaa
acattctctggtatttggactcctgtaaagaatgacttcaaagagttttatgatttatacctttctgatgtagagaaat
ataatggttcggggaaattgtttcccaaaacacctatacctgaaaatgctttttctctttctattattccatggacttc
atttactgggtttaacttaaatatcaataataatagtaattaccttctacccattattacagcaggaaaattcattaat
aaaggtaattcaatatatttaccgctatctttacaggtacatcattctgtttgtgatggttatcatgcaggattgttta
tgaactctattcaggaattgtcagataggcctaatgactggcttttataatatgagataatgccgactgtactttttac
agtcggttttctaatgtcactaacctgccccgttagtcgccattcgccagctgcctcgcgcgtttcggtgatgacggtg
aaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtca
gggcgcgtcagcgggtgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtgtatactggc
ttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggag
aaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggta
tcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggcc
agcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaa
aaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctc
gtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctc
aatgctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttca
gcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagca
gccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggct
acactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatc
cggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaa
gaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagat
tatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaac
ttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcc
tgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacc
cacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaacttt
atccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgtt
gttgccattgccgcaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaa
ggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagtt
ggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttct
gtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaacac
gggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaag
gatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcacc
agcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatac
tcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtat
ttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatc
atgacattaacctataaaaataggcgtatcacgaggccctttcgtcttcaagaattcgcggccgcttctagagtactag
agaattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggc
tcgtgttgtacaataaatgttactagagaaaggtggtgaatactagatgaacatcaaaaaatttgcaaaacaggcgaca
gttctgacatttacaacagcactgcttgcaggcggcgcaacacaagcatttgcaaaagaaacatactagatggatggaa
tttggacacaactgatttttaccctggtgaagaaccttgctacactttggcaatccggcgattttcagttccttgacca
tgaatgccattaccgtattaaacctacgtttagacgcttgaagtggaagtataaaggaaaattttggtgcccgtcttgg
accagcattactggacgtgcgacaaagtcatcacgctccggtgccgtcgaacattcggtccgaaattttgttgggcaag
cgaagtcgtcaggcctgataacgcaaagacaagcggaacagtttattagtcaatacaattaataa