LOCUS BBa_K541915 6109 bp DNA linear 17-May-2024 DEFINITION Multi-host vector pTG262 converted to BioBrick vector wtih LALF protein and SacB signal peptide ACCESSION BBa_K541915 VERSION BBa_K541915.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=BioBrick suffix CDS 1724..2431 /label=repA CDS 3149..3919 /label=neo CDS 4444..5094 /label=cat misc_feature 5543..5564 /label=BioBrick prefix misc_feature 1..5564 /label=BBa_I742123 misc_feature 5573..5669 /label=BBa_K143012 misc_feature 5573..5689 /label=BBa_K143053 promoter 5635..5640 /label=Sigma A-35 promoter 5658..5663 /label=Sigma A -10 RBS 5678..5689 /label=rbs misc_feature 5678..5689 /label=BBa_K143021 misc_feature 5696..5791 /label=SacB CDS 5798..6109 /label=LALF misc_feature 5573..6109 /label=BBa_K541515 ORIGIN 1 tactagtagc ggccgctgca ggcatgcaag cttggcactg gccgtcgttt tacaacgtcg 61 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc 121 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct 181 gaatggcgaa tgctggacta aaaggcatgc aatttcataa tcaaagagag cgaaaaagta 241 gaacgaatga tgatattgac catgagcgaa cacgtgaaaa ttatgatttg aaaaatgata 301 aaaatattga ttacaacgaa cgtgtcaaag aaattattga atcacaaaaa acaggtacaa 361 gaaaaacgga ggaaagatgc tgttcttgta aatgagttgc tagtaacatc tgaccgagat 421 ttttttgagc aactggatca attccttaag gaacgtacag acggcttaaa agcctttaaa 481 aacgttttta aggggtttgt agacaaggta aaggataaaa cagcacaatt ccaagaaaaa 541 cacgatttag aacctaaaaa gaacgaattt gaactaactc ataaccgaga ggtaaaaaaa 601 gaacgaagtc gatgctgttt ggcaaaaaaa gaaaaagtga ttaatttata ttttatttat 661 ggcgctaatt tattagcgct ttttttgttg tcggctagcc gacttctgat acattttttt 721 aagcacaaaa accacccaat tttggagtgg tgtgtaagtg cgcattgttc gaaaaatcga 781 actatgattt atttttgctg ttgtatttat ttttcatctt ttgggttttg gttttgtttt 841 ttgttgctat cgtagtttat ttgcttttta agggctctat ttttcgttct acggcatttt 901 tataatttgc caatataatt tatcgatttt ttattaaaac gtctcaaaat cgtttctgag 961 acgttttagc gtttatttcg tttagttatc ggcataatcg ttaaaacagg cgttatcgta 1021 gcgtaaaagc ccttgagcgt agcgtgcttt gcagcgaaga tgttgtctgt tagattatga 1081 aagccgatga ctgaatgaaa taataagcgc agcgtccttc tatttcggtt ggaggaggct 1141 caagggagtt tgagggaatg aaattccctc atgggtttga ttttaaaaat tgcttgcaat 1201 tttgccgagc ggtagcgctg gaaaaatttt tgaaaaaaat ttggaatttg gaaaaaaatg 1261 gggggaaagg aagcgaattt tgcttccgta ctacgacccc ccattaagtg ccgagtgcca 1321 atttttgtgc caaaaacgct ctatcccaac tggctcaagg gtttgagggg tttttcaatc 1381 gccaacgaat cgccaacgtt ttcgccaacg ttttttataa atctatattt aagtagcttt 1441 attgttgttt ttatgattac aaagtgatac actaatttta taaaattatt tgattggagt 1501 tttttaaatg gtgatttcag aatcgaaaaa aagagttatg atttctctga caaaagagca 1561 agataaaaaa ttaacagata tggcgaaaca aaaaggtttt tcaaaatctg cggttgcggc 1621 gttagctata gaagaatatg caagaaagga atcagaataa aaaaaataag cgaaagctcg 1681 cgtttttaga aggatacgag ttttcgctac ttgtttttga taaggtaata tatcatggct 1741 attaaatact aaagctagaa attttggatt tttattatat cctgactcaa ttcctaatga 1801 ttggaaagaa aaattagaga gtttgggcgt atctatggct gtcagtcctt tacacgatat 1861 ggacgaaaaa aaagataaag atacatggaa tagtagtgat gttatacgaa atggaaagca 1921 ctataaaaaa ccacactatc acgttatata tattgcacga aatcctgtaa caatagaaag 1981 cgttaggaac aagattaagc gaaaattggg gaatagttca gttgctcatg ttgagatact 2041 tgattatatc aaaggttcat atgaatattt gactcatgaa tcaaaggacg ctattgctaa 2101 gaataaacat atatacgaca aaaaagatat tttgaacatt aatgattttg atattgaccg 2161 ctatataaca cttgatgaaa gccaaaaaag agaattgaag aatttacttt tagatatagt 2221 ggatgactat aatttggtaa atacaaaaga tttaatggct tttattcgcc ttaggggagc 2281 ggagtttgga attttaaata cgaatgatgt aaaagatatt gtttcaacaa actctagcgc 2341 ctttagatta tggtttgagg gcaattatca gtgtggatat agagcaagtt atgcaaaggt 2401 tcttgatgct gaaacggggg aaataaaatg acaaacaaag aaaaagagtt atttgctgaa 2461 aatgaggaat taaaaaaaga aattaaggac ttaaaagagc gtattgaaag atacagagaa 2521 atggaagttg aattaagtac aacaatagat ttattgagag gagggattat tgaataaata 2581 aaagcccccc tgacgaaagt cgaagggggc ttttattttg gtttgatgtt gcgattaata 2641 gcaatacgat tgcaataaac aaaatgatcg accgaaaaat aaatataaat cggatataca 2701 atcggcaatt gacgaaactg caaaatatcc tgtaaaggat acggatttta tgaccgatga 2761 tgaagaaaag aatttgaaac gtttgtctga tttggaggaa ggtttacacc gtaaaaggtt 2821 aatctcctat ggtggtttgt taaaagaaat acataaaaaa ttaaaccttg atgacacaga 2881 agaaggcgat ttgattcata cagatgatga cgaaaaagcc gatgaagatg gattttctat 2941 tattgcaatg tggaattggg aacggaaaaa ttattttatt aaagagtagt tcaacaaacg 3001 ggccagtttg ttgaagatta gatgctataa ttgttattaa aaggattgaa ggatgcttag 3061 gaagacgagt tattaatagc tgaataagaa cggtgctctc caaatattct tatttagaaa 3121 agcaaatcta aaattatctg aaaagggaat gagaatagtg aatggaccaa taataatgac 3181 tagagaagaa agaatgaaga ttgttcatga aattaaggaa cgaatattgg ataaatatgg 3241 ggatgatgtt aaggctattg gtgtttatgg ctctcttggt cgtcagactg atgggcccta 3301 ttcggatatt gagatgatgt gtgtcatgtc aacagaggaa gcagagttca gccatgaatg 3361 gacaaccggt gagtggaagg tggaagtgaa ttttgatagc gaagagattc tactagatta 3421 tgcatctcag gtggaatcag attggccgct tacacatggt caatttttct ctattttgcc 3481 gatttatgat tcaggtggat acttagagaa agtgtatcaa actgctaaat cggtagaagc 3541 ccaaacgttc cacgatgcga tttgtgccct tatcgtagaa gagctgtttg aatatgcagg 3601 caaatggcgt aatattcgtg tgcaaggacc gacaacattt ctaccatcct tgactgtaca 3661 ggtagcaatg gcaggtgcca tgttgattgg tctgcatcat cgcatctgtt atacgacgag 3721 cgcttcggtc ttaactgaag cagttaagca atcagatctt ccttcaggtt atgaccatct 3781 gtgccagttc gtaatgtctg gtcaactttc cgactctgag aaacttctgg aatcgctaga 3841 gaatttctgg aatgggattc aggagtggac agaacgacac ggatatatag tggatgtgtc 3901 aaaacgcata ccattttgaa cgatgacctc taataattgt taatcatgtt ggttacgtat 3961 ttattaactt ctcctagtat tagtaattat catggctgtc atggcgcatt aacggaataa 4021 agggtgtgct taaatcgggc cattttgcgt aataagaaaa aggattaatt atgagcgaat 4081 tgaattaata ataaggtaat agatttacat tagaaaatga aaggggattt tatgcgtgag 4141 aatgttacag tctatcccgg caatagttac ccttattatc aagataagaa agaaaaggat 4201 ttttcgctac gctcaaatcc tttaaaaaaa cacaaaagac cacatttttt aatgtggtct 4261 ttattcttca actaaagcac ccattagttc aacaaacgaa aattggataa agtgggatat 4321 ttttaaaata tatatttatg ttacagtaat attgactttt aaaaaaggat tgattctaat 4381 gaagaaagca gacaagtaag cctcctaaat tcactttaga taaaaattta ggaggcatat 4441 caaatgaact ttaataaaat tgatttagac aattggaaga gaaaagagat atttaatcat 4501 tatttgaacc aacaaacgac ttttagtata accacagaaa ttgatattag tgttttatac 4561 cgaaacataa aacaagaagg atataaattt taccctgcat ttattttctt agtgacaagg 4621 gtgataaact caaatacagc ttttagaact ggttacaata gcgacggaga gttaggttat 4681 tgggataagt tagagccact ttatacaatt tttgatggtg tatctaaaac attctctggt 4741 atttggactc ctgtaaagaa tgacttcaaa gagttttatg atttatacct ttctgatgta 4801 gagaaatata atggttcggg gaaattgttt cccaaaacac ctatacctga aaatgctttt 4861 tctctttcta ttattccatg gacttcattt actgggttta acttaaatat caataataat 4921 agtaattacc ttctacccat tattacagca ggaaaattca ttaataaagg taattcaata 4981 tatttaccgc tatctttaca ggtacatcat tctgtttgtg atggttatca tgcaggattg 5041 tttatgaact ctattcagga attgtcagat aggcctaatg actggctttt ataatatgag 5101 ataatgccga ctgtactttt tacagtcggt tttctaaaac gatacattaa taggtacgaa 5161 aaagcaactt tttttgcgct taaaaccagt cataccaata acttaagggt aactagcctc 5221 gccggaaaga gcgaaaatgc ctcacatttg tgccacctaa aaaggagcga tttacatatg 5281 agttatgcag tttgtagaat gcaaaaagtg aaatcagcca atacgcaaac cgcctctccc 5341 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 5401 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 5461 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 5521 aaacagctat gaccatgatt acgaattcgc ggccgcttct agagtactag agaattttgt 5581 caaaataatt ttattgacaa cgtcttatta acgttgatat aatttaaatt ttatttgaca 5641 aaaatgggct cgtgttgtac aataaatgtt actagagaaa ggtggtgaat actagatgaa 5701 catcaaaaaa tttgcaaaac aggcgacagt tctgacattt acaacagcac tgcttgcagg 5761 cggcgcaaca caagcatttg caaaagaaac atactagatg gatggaattt ggacacaact 5821 gatttttacc ctggtgaaga accttgctac actttggcaa tccggcgatt ttcagttcct 5881 tgaccatgaa tgccattacc gtattaaacc tacgtttaga cgcttgaagt ggaagtataa 5941 aggaaaattt tggtgcccgt cttggaccag cattactgga cgtgcgacaa agtcatcacg 6001 ctccggtgcc gtcgaacatt cggtccgaaa ttttgttggg caagcgaagt cgtcaggcct 6061 gataacgcaa agacaagcgg aacagtttat tagtcaatac aattaataa //