LOCUS       BBa_K542001              828 bp    DNA     linear       22-Dec-2024
DEFINITION  pLacI Regulated Lumazine Synthase
ACCESSION   BBa_K542001
VERSION     BBa_K542001.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..691
                     /label=BBa_K249002
     stem_loop       711..754
                     /label=stem_loop
     misc_feature    700..779
                     /label=BBa_B0010
     terminator      700..779
                     /label=BBa_B0010
     stem_loop       795..814
                     /label=T7 TE
     misc_feature    821..821
                     /label=stop
     polyA_site      815..828
                     /label=polya
     misc_feature    788..828
                     /label=BBa_B0012
     terminator      788..828
                     /label=BBa_B0012
     terminator      700..828
                     /label=BBa_B0015
     misc_feature    227..828
                     /label=BBa_K542000
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc agatttatga
      241 aggcaaactg accgcggaag gcctgcgctt tggcattgtg gcgagccgct ttaaccatgc
      301 gctggtggat cgcctggtgg aaggcgcgat tgattgcatt gtgcgccatg gtggtcgcga
      361 agaagatatt accctggtgc gcgtgccggg cagctgggaa attccggtgg cggcgggcga
      421 actggcgcgc aaagaagata ttgatgcggt gattgcgatt ggcgtgctga ttgaaggcgc
      481 ggaaccgcat tttgattata ttgcgagcga agtgagcaaa ggcctggcga acctgagcct
      541 ggaactgcgc aaaccgatta cctttggcgt gattaccgcg gatgaactgg aagaagcgat
      601 tgaacgcgcg ggcaccaaac atggcaacaa aggctgggaa gcggcgctga gcgcgattga
      661 aatggcgaac ctgtttaaaa gcctgcgcta gtactagagc caggcatcaa ataaaacgaa
      721 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
      781 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//