##gff-version 3
##sequence-region BBa_K542001 1 828
BBa_K542001	.	engineered_region	227	828	.	+	0	ID=BBa_K542000;Name=BBa_K542000
BBa_K542001	.	terminator	700	828	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K542000
BBa_K542001	.	terminator	788	828	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K542001	.	stem_loop	795	814	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K542001	.	polyA_site	815	828	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K542001	.	stop_codon	821	821	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K542001	.	engineered_region	788	828	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K542001	.	terminator	700	779	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K542001	.	stem_loop	711	754	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K542001	.	engineered_region	700	779	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K542001	.	CDS	227	691	.	+	0	ID=BBa_K249002;Name=BBa_K249002;Parent=BBa_K542000
BBa_K542001	.	engineered_region	1	220	.	+	0	ID=BBa_J04500;Name=BBa_J04500
BBa_K542001	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500
BBa_K542001	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K542001	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K542001	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K542001	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K542001	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K542001	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K542001	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K542001	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500
BBa_K542001	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K542001
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgcagattta
tgaaggcaaactgaccgcggaaggcctgcgctttggcattgtggcgagccgctttaaccatgcgctggtggatcgcctg
gtggaaggcgcgattgattgcattgtgcgccatggtggtcgcgaagaagatattaccctggtgcgcgtgccgggcagct
gggaaattccggtggcggcgggcgaactggcgcgcaaagaagatattgatgcggtgattgcgattggcgtgctgattga
aggcgcggaaccgcattttgattatattgcgagcgaagtgagcaaaggcctggcgaacctgagcctggaactgcgcaaa
ccgattacctttggcgtgattaccgcggatgaactggaagaagcgattgaacgcgcgggcaccaaacatggcaacaaag
gctgggaagcggcgctgagcgcgattgaaatggcgaacctgtttaaaagcctgcgctagtactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttata