LOCUS       BBa_K542004             1814 bp    DNA     linear       26-Dec-2024
DEFINITION  pLacI Regulated Lumazine Synthase with Transciptional Terminator 
            and pBAD
ACCESSION   BBa_K542004
VERSION     BBa_K542004.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..691
                     /label=BBa_K249002
     stem_loop       711..754
                     /label=stem_loop
     misc_feature    700..779
                     /label=BBa_B0010
     terminator      700..779
                     /label=BBa_B0010
     stem_loop       795..814
                     /label=T7 TE
     misc_feature    821..821
                     /label=stop
     polyA_site      815..828
                     /label=polya
     misc_feature    788..828
                     /label=BBa_B0012
     terminator      788..828
                     /label=BBa_B0012
     terminator      700..828
                     /label=BBa_B0015
     misc_feature    227..828
                     /label=BBa_K542000
     misc_feature    1..828
                     /label=BBa_K542001
     promoter        837..966
                     /label=BBa_I13453
     misc_feature    979..982
                     /label=conserved
     RBS             975..986
                     /label=BBa_B0034
     CDS             996..1612
                     /label=tetR
     misc_feature    1613..1646
                     /label=SsrA
     misc_feature    1653..1677
                     /label=Help:Barcodes
     CDS             993..1652
                     /label=BBa_C0040
     stem_loop       1697..1740
                     /label=stem_loop
     misc_feature    1686..1765
                     /label=BBa_B0010
     terminator      1686..1765
                     /label=BBa_B0010
     stem_loop       1781..1800
                     /label=T7 TE
     misc_feature    1807..1807
                     /label=stop
     polyA_site      1801..1814
                     /label=polya
     misc_feature    1774..1814
                     /label=BBa_B0012
     terminator      1774..1814
                     /label=BBa_B0012
     misc_feature    975..1814
                     /label=BBa_P0440
     misc_feature    837..1814
                     /label=BBa_K542003
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc agatttatga
      241 aggcaaactg accgcggaag gcctgcgctt tggcattgtg gcgagccgct ttaaccatgc
      301 gctggtggat cgcctggtgg aaggcgcgat tgattgcatt gtgcgccatg gtggtcgcga
      361 agaagatatt accctggtgc gcgtgccggg cagctgggaa attccggtgg cggcgggcga
      421 actggcgcgc aaagaagata ttgatgcggt gattgcgatt ggcgtgctga ttgaaggcgc
      481 ggaaccgcat tttgattata ttgcgagcga agtgagcaaa ggcctggcga acctgagcct
      541 ggaactgcgc aaaccgatta cctttggcgt gattaccgcg gatgaactgg aagaagcgat
      601 tgaacgcgcg ggcaccaaac atggcaacaa aggctgggaa gcggcgctga gcgcgattga
      661 aatggcgaac ctgtttaaaa gcctgcgcta gtactagagc caggcatcaa ataaaacgaa
      721 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
      781 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacat
      841 tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca taagattagc
      901 ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccatacc gtttttttgg
      961 gctagctact agagaaagag gagaaatact agatgtccag attagataaa agtaaagtga
     1021 ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac
     1081 tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg
     1141 ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt
     1201 tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt
     1261 tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt
     1321 atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg
     1381 cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag
     1441 agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat
     1501 tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc
     1561 ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tccgctgcaa
     1621 acgacgaaaa ctacgcttta gtagcttaat aacactgata gtgctagtgt agatcactac
     1681 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     1741 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     1801 tttctgcgtt tata
//