LOCUS       BBa_K542005             1755 bp    DNA     linear       16-Jun-2024
DEFINITION  pTet Regulated Arg-tagged ECFP and EYFP (FRET Reporter)
ACCESSION   BBa_K542005
VERSION     BBa_K542005.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..83
                     /label=start
     misc_feature    834..836
                     /label=stop
     misc_feature    805..833
                     /label=Arginine Tag
     misc_feature    81..836
                     /label=BBa_K331002
     misc_feature    1..836
                     /label=BBa_K331033
     misc_feature    849..852
                     /label=conserved
     RBS             845..856
                     /label=BBa_B0034
     misc_feature    863..1618
                     /label=BBa_K249006
     stem_loop       1638..1681
                     /label=stem_loop
     misc_feature    1627..1706
                     /label=BBa_B0010
     terminator      1627..1706
                     /label=BBa_B0010
     stem_loop       1722..1741
                     /label=T7 TE
     misc_feature    1748..1748
                     /label=stop
     polyA_site      1742..1755
                     /label=polya
     misc_feature    1715..1755
                     /label=BBa_B0012
     terminator      1715..1755
                     /label=BBa_B0012
     misc_feature    845..1755
                     /label=BBa_K331035
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc
      121 ccatcctggt cgagctggac ggcgacgtga acggccacaa gttcagcgtg tccggcgagg
      181 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      241 tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc
      301 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      361 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      421 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      481 acggcaacat cctggggcac aagctggagt acaactacat cagccacaac gtctatatca
      541 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
      601 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      661 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
      721 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      781 tggacgagct gtacaagcac tagagccgcc gccgccgccg ccgccgccgc cgctaatact
      841 agagaaagag gagaaatact agatggtgag caagggcgag gagctgttca ccggggtggt
      901 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
      961 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     1021 gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc aatgcttcgc
     1081 ccgctacccc gaccacatga agctgcacga cttcttcaag tccgccatgc ccgaaggcta
     1141 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1201 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1261 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1321 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     1381 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1441 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa
     1501 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     1561 catggacgag ctgtacaagc actagagccg ccgccgccgc cgccgccgcc gccgctaata
     1621 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1681 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1741 ctttctgcgt ttata
//