##gff-version 3
##sequence-region BBa_K542005 1 1755
BBa_K542005	.	engineered_region	1	836	.	+	0	ID=BBa_K331033;Name=BBa_K331033
BBa_K542005	.	engineered_region	81	836	.	+	0	ID=BBa_K331002;Name=BBa_K331002;Parent=BBa_K331033
BBa_K542005	.	sequence_feature	805	833	.	+	0	ID=annotation2071687;Name=Arginine Tag;Parent=BBa_K331002
BBa_K542005	.	start_codon	81	83	.	+	0	ID=annotation2071685;Name=start;Parent=BBa_K331002
BBa_K542005	.	stop_codon	834	836	.	+	0	ID=annotation2071686;Name=stop;Parent=BBa_K331002
BBa_K542005	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K331033
BBa_K542005	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K542005	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K331033
BBa_K542005	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K542005	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K542005	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K542005	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K542005	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K542005	.	engineered_region	845	1755	.	+	0	ID=BBa_K331035;Name=BBa_K331035
BBa_K542005	.	engineered_region	863	1618	.	+	0	ID=BBa_K249006;Name=BBa_K249006;Parent=BBa_K331035
BBa_K542005	.	ribosome_entry_site	845	856	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K331035
BBa_K542005	.	conserved_region	849	852	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K542005	.	terminator	1715	1755	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K331035
BBa_K542005	.	stem_loop	1722	1741	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K542005	.	stop_codon	1748	1748	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K542005	.	polyA_site	1742	1755	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K542005	.	engineered_region	1715	1755	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K542005	.	terminator	1627	1706	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K331035
BBa_K542005	.	stem_loop	1638	1681	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K542005	.	engineered_region	1627	1706	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K542005
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccac
aagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggca
agctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacat
gaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggc
aactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttca
aggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagca
gaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccag
cagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaag
accccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagct
gtacaagcactagagccgccgccgccgccgccgccgccgccgctaatactagagaaagaggagaaatactagatggtga
gcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcag
cgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgccc
gtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgc
acgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaa
gacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggac
ggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacg
gcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacac
ccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaac
gagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagc
actagagccgccgccgccgccgccgccgccgccgctaatactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttata