##gff-version 3
##sequence-region BBa_K542006 1 2741
BBa_K542006	.	engineered_region	1	978	.	+	0	ID=BBa_K542003;Name=BBa_K542003
BBa_K542006	.	engineered_region	139	978	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_K542003
BBa_K542006	.	CDS	157	816	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_K542006	.	engineered_tag	817	841	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K542006	.	CDS	160	776	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K542006	.	tag	777	810	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K542006	.	terminator	850	929	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_K542006	.	stem_loop	861	904	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K542006	.	engineered_region	850	929	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K542006	.	terminator	938	978	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_K542006	.	polyA_site	965	978	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K542006	.	stem_loop	945	964	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K542006	.	stop_codon	971	971	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K542006	.	engineered_region	938	978	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K542006	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_K542006	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K542006	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K542003
BBa_K542006	.	engineered_region	987	2741	.	+	0	ID=BBa_K542005;Name=BBa_K542005
BBa_K542006	.	engineered_region	1831	2741	.	+	0	ID=BBa_K331035;Name=BBa_K331035;Parent=BBa_K542005
BBa_K542006	.	terminator	2613	2692	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K331035
BBa_K542006	.	stem_loop	2624	2667	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K542006	.	engineered_region	2613	2692	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K542006	.	engineered_region	1849	2604	.	+	0	ID=BBa_K249006;Name=BBa_K249006;Parent=BBa_K331035
BBa_K542006	.	ribosome_entry_site	1831	1842	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K331035
BBa_K542006	.	conserved_region	1835	1838	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K542006	.	terminator	2701	2741	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K331035
BBa_K542006	.	polyA_site	2728	2741	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K542006	.	stem_loop	2708	2727	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K542006	.	stop_codon	2734	2734	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K542006	.	engineered_region	2701	2741	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K542006	.	engineered_region	987	1822	.	+	0	ID=BBa_K331033;Name=BBa_K331033;Parent=BBa_K542005
BBa_K542006	.	engineered_region	1067	1822	.	+	0	ID=BBa_K331002;Name=BBa_K331002;Parent=BBa_K331033
BBa_K542006	.	stop_codon	1820	1822	.	+	0	ID=annotation2071686;Name=stop;Parent=BBa_K331002
BBa_K542006	.	start_codon	1067	1069	.	+	0	ID=annotation2071685;Name=start;Parent=BBa_K331002
BBa_K542006	.	sequence_feature	1791	1819	.	+	0	ID=annotation2071687;Name=Arginine Tag;Parent=BBa_K331002
BBa_K542006	.	ribosome_entry_site	1049	1060	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K331033
BBa_K542006	.	conserved_region	1053	1056	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K542006	.	promoter	987	1040	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K331033
BBa_K542006	.	promoter	1029	1034	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K542006	.	non_covalent_binding_site	1012	1030	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K542006	.	promoter	1006	1011	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K542006	.	engineered_region	987	1040	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K542006	.	non_covalent_binding_site	987	1005	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
>BBa_K542006
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
gtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacc
cgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacg
ccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacg
taataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctaca
gaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatg
cactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaaggga
aacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagcc
ttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacg
aaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatag
agatactgagcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgc
ccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccaccta
cggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacc
tggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggct
acgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacac
cctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaac
tacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaaca
tcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccga
caaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttc
gtgaccgccgccgggatcactctcggcatggacgagctgtacaagcactagagccgccgccgccgccgccgccgccgcc
gctaatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctg
gtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagc
tgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcct
gcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccag
gagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtga
accgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacag
ccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggac
ggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccact
acctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgc
cgccgggatcactctcggcatggacgagctgtacaagcactagagccgccgccgccgccgccgccgccgccgctaatac
tagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac
gctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata