LOCUS BBa_K542008 3577 bp DNA linear 05-Nov-2024 DEFINITION pLacI Regulated Lumazine Synthase and pBAD Inverse-Regulated Arg-tagged ECFP and EYFP ACCESSION BBa_K542008 VERSION BBa_K542008.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 1..220 /label=BBa_J04500 CDS 227..691 /label=BBa_K249002 stem_loop 711..754 /label=stem_loop misc_feature 700..779 /label=BBa_B0010 terminator 700..779 /label=BBa_B0010 stem_loop 795..814 /label=T7 TE misc_feature 821..821 /label=stop polyA_site 815..828 /label=polya misc_feature 788..828 /label=BBa_B0012 terminator 788..828 /label=BBa_B0012 terminator 700..828 /label=BBa_B0015 misc_feature 227..828 /label=BBa_K542000 misc_feature 1..828 /label=BBa_K542001 promoter 837..966 /label=BBa_I13453 misc_feature 979..982 /label=conserved RBS 975..986 /label=BBa_B0034 CDS 996..1612 /label=tetR misc_feature 1613..1646 /label=SsrA misc_feature 1653..1677 /label=Help:Barcodes CDS 993..1652 /label=BBa_C0040 stem_loop 1697..1740 /label=stem_loop misc_feature 1686..1765 /label=BBa_B0010 terminator 1686..1765 /label=BBa_B0010 stem_loop 1781..1800 /label=T7 TE misc_feature 1807..1807 /label=stop polyA_site 1801..1814 /label=polya misc_feature 1774..1814 /label=BBa_B0012 terminator 1774..1814 /label=BBa_B0012 misc_feature 975..1814 /label=BBa_P0440 misc_feature 837..1814 /label=BBa_K542003 misc_feature 1..1814 /label=BBa_K542004 misc_feature 1823..1841 /label=TetR 1 misc_feature 1823..1876 /label=BBa_R0040 promoter 1842..1847 /label=-35 misc_feature 1848..1866 /label=TetR 2 promoter 1865..1870 /label=-10 promoter 1823..1876 /label=BBa_R0040 misc_feature 1889..1892 /label=conserved RBS 1885..1896 /label=BBa_B0034 misc_feature 1903..1905 /label=start misc_feature 2656..2658 /label=stop misc_feature 2627..2655 /label=Arginine Tag misc_feature 1903..2658 /label=BBa_K331002 misc_feature 1823..2658 /label=BBa_K331033 misc_feature 2671..2674 /label=conserved RBS 2667..2678 /label=BBa_B0034 misc_feature 2685..3440 /label=BBa_K249006 stem_loop 3460..3503 /label=stem_loop misc_feature 3449..3528 /label=BBa_B0010 terminator 3449..3528 /label=BBa_B0010 stem_loop 3544..3563 /label=T7 TE misc_feature 3570..3570 /label=stop polyA_site 3564..3577 /label=polya misc_feature 3537..3577 /label=BBa_B0012 terminator 3537..3577 /label=BBa_B0012 misc_feature 2667..3577 /label=BBa_K331035 misc_feature 1823..3577 /label=BBa_K542005 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc agatttatga 241 aggcaaactg accgcggaag gcctgcgctt tggcattgtg gcgagccgct ttaaccatgc 301 gctggtggat cgcctggtgg aaggcgcgat tgattgcatt gtgcgccatg gtggtcgcga 361 agaagatatt accctggtgc gcgtgccggg cagctgggaa attccggtgg cggcgggcga 421 actggcgcgc aaagaagata ttgatgcggt gattgcgatt ggcgtgctga ttgaaggcgc 481 ggaaccgcat tttgattata ttgcgagcga agtgagcaaa ggcctggcga acctgagcct 541 ggaactgcgc aaaccgatta cctttggcgt gattaccgcg gatgaactgg aagaagcgat 601 tgaacgcgcg ggcaccaaac atggcaacaa aggctgggaa gcggcgctga gcgcgattga 661 aatggcgaac ctgtttaaaa gcctgcgcta gtactagagc caggcatcaa ataaaacgaa 721 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 781 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacat 841 tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca taagattagc 901 ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccatacc gtttttttgg 961 gctagctact agagaaagag gagaaatact agatgtccag attagataaa agtaaagtga 1021 ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac 1081 tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg 1141 ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt 1201 tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt 1261 tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt 1321 atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg 1381 cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag 1441 agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat 1501 tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc 1561 ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tccgctgcaa 1621 acgacgaaaa ctacgcttta gtagcttaat aacactgata gtgctagtgt agatcactac 1681 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 1741 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 1801 tttctgcgtt tatatactag agtccctatc agtgatagag attgacatcc ctatcagtga 1861 tagagatact gagcactact agagaaagag gagaaatact agatggtgag caagggcgag 1921 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt gaacggccac 1981 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag 2041 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc 2101 tggggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag 2161 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac 2221 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg 2281 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac 2341 atcagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc 2401 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac 2461 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc 2521 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc 2581 gccgccggga tcactctcgg catggacgag ctgtacaagc actagagccg ccgccgccgc 2641 cgccgccgcc gccgctaata ctagagaaag aggagaaata ctagatggtg agcaagggcg 2701 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc 2761 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga 2821 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg 2881 gctacggcct gcaatgcttc gcccgctacc ccgaccacat gaagctgcac gacttcttca 2941 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca 3001 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc 3061 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact 3121 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact 3181 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga 3241 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt 3301 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga 3361 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gcactagagc cgccgccgcc 3421 gccgccgccg ccgccgctaa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 3481 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 3541 actggctcac cttcgggtgg gcctttctgc gtttata //