##gff-version 3
##sequence-region BBa_K542008 1 3577
BBa_K542008	.	engineered_region	1823	3577	.	+	0	ID=BBa_K542005;Name=BBa_K542005
BBa_K542008	.	engineered_region	2667	3577	.	+	0	ID=BBa_K331035;Name=BBa_K331035;Parent=BBa_K542005
BBa_K542008	.	terminator	3537	3577	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K331035
BBa_K542008	.	polyA_site	3564	3577	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K542008	.	stem_loop	3544	3563	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K542008	.	stop_codon	3570	3570	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K542008	.	engineered_region	3537	3577	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K542008	.	terminator	3449	3528	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K331035
BBa_K542008	.	stem_loop	3460	3503	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K542008	.	engineered_region	3449	3528	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K542008	.	engineered_region	2685	3440	.	+	0	ID=BBa_K249006;Name=BBa_K249006;Parent=BBa_K331035
BBa_K542008	.	ribosome_entry_site	2667	2678	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K331035
BBa_K542008	.	conserved_region	2671	2674	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K542008	.	engineered_region	1823	2658	.	+	0	ID=BBa_K331033;Name=BBa_K331033;Parent=BBa_K542005
BBa_K542008	.	promoter	1823	1876	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K331033
BBa_K542008	.	promoter	1865	1870	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K542008	.	non_covalent_binding_site	1823	1841	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K542008	.	engineered_region	1823	1876	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K542008	.	non_covalent_binding_site	1848	1866	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K542008	.	promoter	1842	1847	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K542008	.	engineered_region	1903	2658	.	+	0	ID=BBa_K331002;Name=BBa_K331002;Parent=BBa_K331033
BBa_K542008	.	stop_codon	2656	2658	.	+	0	ID=annotation2071686;Name=stop;Parent=BBa_K331002
BBa_K542008	.	start_codon	1903	1905	.	+	0	ID=annotation2071685;Name=start;Parent=BBa_K331002
BBa_K542008	.	sequence_feature	2627	2655	.	+	0	ID=annotation2071687;Name=Arginine Tag;Parent=BBa_K331002
BBa_K542008	.	ribosome_entry_site	1885	1896	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K331033
BBa_K542008	.	conserved_region	1889	1892	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K542008	.	engineered_region	1	1814	.	+	0	ID=BBa_K542004;Name=BBa_K542004
BBa_K542008	.	engineered_region	1	828	.	+	0	ID=BBa_K542001;Name=BBa_K542001;Parent=BBa_K542004
BBa_K542008	.	engineered_region	227	828	.	+	0	ID=BBa_K542000;Name=BBa_K542000;Parent=BBa_K542001
BBa_K542008	.	CDS	227	691	.	+	0	ID=BBa_K249002;Name=BBa_K249002;Parent=BBa_K542000
BBa_K542008	.	terminator	700	828	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K542000
BBa_K542008	.	terminator	788	828	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K542008	.	polyA_site	815	828	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K542008	.	stem_loop	795	814	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K542008	.	stop_codon	821	821	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K542008	.	engineered_region	788	828	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K542008	.	terminator	700	779	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K542008	.	stem_loop	711	754	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K542008	.	engineered_region	700	779	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K542008	.	engineered_region	1	220	.	+	0	ID=BBa_J04500;Name=BBa_J04500;Parent=BBa_K542001
BBa_K542008	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500
BBa_K542008	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K542008	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K542008	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K542008	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K542008	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K542008	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K542008	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K542008	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500
BBa_K542008	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K542008	.	engineered_region	837	1814	.	+	0	ID=BBa_K542003;Name=BBa_K542003;Parent=BBa_K542004
BBa_K542008	.	engineered_region	975	1814	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_K542003
BBa_K542008	.	terminator	1774	1814	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_K542008	.	polyA_site	1801	1814	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K542008	.	stem_loop	1781	1800	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K542008	.	stop_codon	1807	1807	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K542008	.	engineered_region	1774	1814	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K542008	.	terminator	1686	1765	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_K542008	.	stem_loop	1697	1740	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K542008	.	engineered_region	1686	1765	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K542008	.	ribosome_entry_site	975	986	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_K542008	.	conserved_region	979	982	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K542008	.	CDS	993	1652	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_K542008	.	tag	1613	1646	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K542008	.	CDS	996	1612	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K542008	.	engineered_tag	1653	1677	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K542008	.	promoter	837	966	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K542003
>BBa_K542008
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgcagattta
tgaaggcaaactgaccgcggaaggcctgcgctttggcattgtggcgagccgctttaaccatgcgctggtggatcgcctg
gtggaaggcgcgattgattgcattgtgcgccatggtggtcgcgaagaagatattaccctggtgcgcgtgccgggcagct
gggaaattccggtggcggcgggcgaactggcgcgcaaagaagatattgatgcggtgattgcgattggcgtgctgattga
aggcgcggaaccgcattttgattatattgcgagcgaagtgagcaaaggcctggcgaacctgagcctggaactgcgcaaa
ccgattacctttggcgtgattaccgcggatgaactggaagaagcgattgaacgcgcgggcaccaaacatggcaacaaag
gctgggaagcggcgctgagcgcgattgaaatggcgaacctgtttaaaagcctgcgctagtactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacactttgct
atgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccata
ccgtttttttgggctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacag
cgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcag
cctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccata
ctcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttact
aagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaatta
gcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggtt
gcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattatt
acgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcgga
ttagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgat
agtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatac
tagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaa
tactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacg
gccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccac
cggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgac
cacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacg
acggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcga
cttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgac
aagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccact
accagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgag
caaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggac
gagctgtacaagcactagagccgccgccgccgccgccgccgccgccgctaatactagagaaagaggagaaatactagat
ggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaag
ttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagc
tgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaa
gctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaac
tacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaagg
aggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaa
gaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcag
aacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagacc
ccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgta
caagcactagagccgccgccgccgccgccgccgccgccgctaatactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttata