BBa_K542008
type: ComponentDefinition
persistent identity: BBa K542008
display id: BBa_K542008
version: 1
was generated by: iGEM to SBOL conversion
description: pLacI Regulated Lumazine Synthase and pBAD Inverse-Regulated Arg-tagged ECFP and EYFP
created: 2011-09-23T11:00:00Z
modified: 2015-05-08T01:12:39Z
mutable provenance: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K542004 BBa_K542004] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K542005 BBa_542005] were both assembled by the Lethbridge 2011 team.
owned by: james
owned by: myers
mutable description: Made by assembling [http://partsregistry.org/wiki/index.php?title=Part:BBa_K542004 BBa_K542004] with [http://partsregistry.org/wiki/index.php?title=Part:BBa_K542005 BBa_542005]. The expression of Lumazine Synthase and the fluorescence proteins are independently regulated by two separate promoters. Lumazine Synthase is regulated by the pLacI promoter and the fluorescent proteins are regulated by the pBAD inverter. Since the two are independently controlled, Lumazine Synthase microcompartments may be formed in the presence or absence of the fluorescent proteins (ie. absence or presence of arabinose, respectively). Because the fluorescent proteins are positively tagged (ie. arginines) and the Lumazine Synthase is mutated with a negative interior ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K249002 BBa_249002]/[http://2009.igem.org/Team:Lethbridge/Modeling Lethbridge 2009 Modeling]), ECFP and EYFP should be targeted into the microcompartments. ECFP and EYFP is a well known FRET pair. Fluorescence/F??rster Resonance Energy Transfer(FRET) is a distance-dependent phenomenon in which the excitation of a donor fluorophore leads to emission by an acceptor fluorophore; this occurs if the two fluorophores are within a certain distance to each other. The distance is dependent on the FRET pair used. The emission spectrum of the donor fluorophore must overlap with the excitation spectrum of the acceptor fluorophore for FRET to occur. FRET is explained in further detail on the [http://2009.igem.org/Team:Lethbridge/Project#FRET Lethbridge 2009 Wiki]. This phenomenon will allow for characterizing co-localization within the Lumazine Synthase microcompartment. If FRET is observed, it is suggestive that both ECFP and EYFP were co-localized into the microcompartment. In the design of this "Co-localization Testing Construct" control experiments were also taken into consideration. In the presence of arabinose (cease fluorescent protein expression), no FRET should be observed be…
discontinued: false
dominant: false
experience: experience/Works
group u list: _709_
m user id: 0
owner id: 6015
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: The Lethbridge 2011 team took two approaches in assembling this "Co-localization Testing Construct".
star: false
creator: Anthony Vuong
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_K542005
component: BBa_K542004
sequence annotation: BBa_K542004
sequence annotation: BBa_K542005
sequence: https://synbiohub.org/public/igem/BBa_K542008_sequence/1

BBa_K542005
type: Component
persistent identity: component2138916
display id: component2138916
version: 1
top level: BBa_K542008
definition: BBa_K542005
access: public

BBa_K542004
type: Component
persistent identity: component2138893
display id: component2138893
version: 1
top level: BBa_K542008
definition: BBa_K542004
access: public

BBa_K542004
type: SequenceAnnotation
persistent identity: annotation2138893
display id: annotation2138893
version: 1
top level: BBa_K542008
location: https://synbiohub.org/public/igem/BBa_K542008/annotation2138893/range2138893/1
component: BBa_K542004

https://synbiohub.org/public/igem/BBa_K542008/annotation2138893/range2138893/1
type: Range
persistent identity: range2138893
display id: range2138893
version: 1
top level: BBa_K542008
direction: direction/reverse
start: 1
end: 1814
orientation: inline

BBa_K542005
type: SequenceAnnotation
persistent identity: annotation2138916
display id: annotation2138916
version: 1
top level: BBa_K542008
location: https://synbiohub.org/public/igem/BBa_K542008/annotation2138916/range2138916/1
component: BBa_K542005

https://synbiohub.org/public/igem/BBa_K542008/annotation2138916/range2138916/1
type: Range
persistent identity: range2138916
display id: range2138916
version: 1
top level: BBa_K542008
direction: direction/reverse
start: 1823
end: 3577
orientation: inline

BBa_K542004
type: ComponentDefinition
persistent identity: BBa K542004
display id: BBa_K542004
version: 1
was generated by: iGEM to SBOL conversion
description: pLacI Regulated Lumazine Synthase with Transciptional Terminator and pBAD
created: 2011-09-22T11:00:00Z
modified: 2015-05-08T01:12:39Z
mutable provenance: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K542001 BBa_K542001] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K542003 BBa_K542003] were both assembled by the Lethbridge 2011 team.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Made by assembling [http://partsregistry.org/wiki/index.php?title=Part:BBa_K542001 BBa_K542001] with [http://partsregistry.org/wiki/index.php?title=Part:BBa_K542003 BBa_K542003]. Note that the pLacI promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010]) is regulating the Lumazine Synthase gene, not the pBAD promoter ([http://partsregistry.org/Part:BBa_I13453 BBa_I13453]) which is downstream. Therefore, Lumazine Synthase production is IPTG inducible. The microcompartment portion to the "Co-localization Construct".
discontinued: false
dominant: false
experience: experience/Works
group u list: _709_
m user id: 0
owner id: 6015
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The microcompartment portion to the "Co-localization Construct".
star: false
creator: Anthony Vuong
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K542001
component: BBa_K542003
sequence annotation: BBa_K542001
sequence annotation: BBa_K542003
sequence: https://synbiohub.org/public/igem/BBa_K542004_sequence/1

BBa_K542001
type: Component
persistent identity: component2138620
display id: component2138620
version: 1
top level: BBa_K542004
definition: BBa_K542001
access: public

BBa_K542003
type: Component
persistent identity: component2138635
display id: component2138635
version: 1
top level: BBa_K542004
definition: BBa_K542003
access: public

BBa_K542001
type: SequenceAnnotation
persistent identity: annotation2138620
display id: annotation2138620
version: 1
top level: BBa_K542004
location: https://synbiohub.org/public/igem/BBa_K542004/annotation2138620/range2138620/1
component: BBa_K542001

https://synbiohub.org/public/igem/BBa_K542004/annotation2138620/range2138620/1
type: Range
persistent identity: range2138620
display id: range2138620
version: 1
top level: BBa_K542004
direction: direction/reverse
start: 1
end: 828
orientation: inline

BBa_K542003
type: SequenceAnnotation
persistent identity: annotation2138635
display id: annotation2138635
version: 1
top level: BBa_K542004
location: https://synbiohub.org/public/igem/BBa_K542004/annotation2138635/range2138635/1
component: BBa_K542003

https://synbiohub.org/public/igem/BBa_K542004/annotation2138635/range2138635/1
type: Range
persistent identity: range2138635
display id: range2138635
version: 1
top level: BBa_K542004
direction: direction/reverse
start: 837
end: 1814
orientation: inline

BBa_K542005
type: ComponentDefinition
persistent identity: BBa K542005
display id: BBa_K542005
version: 1
was generated by: iGEM to SBOL conversion
description: pTet Regulated Arg-tagged ECFP and EYFP (FRET Reporter)
created: 2011-09-22T11:00:00Z
modified: 2015-05-08T01:12:39Z
mutable provenance: [http://partsregistry.org/Part:BBa_K331033 BBa_K331033] and [http://partsregistry.org/Part:BBa_K331035 BBa_K331035] were both assembled by the Lethbridge 2010 team.
owned by: james
owned by: myers
mutable description: Made by assembling [http://partsregistry.org/Part:BBa_K331033 BBa_K331033] with [http://partsregistry.org/Part:BBa_K331035 BBa_K331035]. The pTet promoter is constitutively "on"; thus, Arg-tagged ECFP and Arg-tagged EYFP will be produced constitutively. By placing this part downstream of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K542003 BBa_K542003], expression of the fluorescence proteins maybe "turned off" in the presence of arabinose. Fluorescence/F??rster Resonance Energy Transfer(FRET) is a distance-dependent phenomenon in which the excitation of a donor fluorophore leads to emission by an acceptor fluorophore; this occurs if the two fluorophores are within a certain distance to each other. The distance is dependent on the FRET pair used. The emission spectrum of the donor fluorophore must overlap with the excitation spectrum of the acceptor fluorophore for FRET to occur. FRET is explained in further detail on the [http://2009.igem.org/Team:Lethbridge/Project#FRET Lethbridge 2009 Wiki]. This phenomenon will allow for characterizing co-localization within the Lumazine Synthase microcompartment.
discontinued: false
dominant: false
experience: experience/Works
group u list: _709_
m user id: 0
owner id: 6015
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: In order to characterize the Lumazine Synthase microcompartment, the fluorescent proteins must be regulated by a different promoter than that of Lumazine.
star: false
creator: Anthony Vuong
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_K331035
component: BBa_K331033
sequence annotation: BBa_K331033
sequence annotation: BBa_K331035
sequence: https://synbiohub.org/public/igem/BBa_K542005_sequence/1

BBa_K331035
type: Component
persistent identity: component2138665
display id: component2138665
version: 1
top level: BBa_K542005
definition: BBa_K331035
access: public

BBa_K331033
type: Component
persistent identity: component2138655
display id: component2138655
version: 1
top level: BBa_K542005
definition: BBa_K331033
access: public

BBa_K331033
type: SequenceAnnotation
persistent identity: annotation2138655
display id: annotation2138655
version: 1
top level: BBa_K542005
location: https://synbiohub.org/public/igem/BBa_K542005/annotation2138655/range2138655/1
component: BBa_K331033

https://synbiohub.org/public/igem/BBa_K542005/annotation2138655/range2138655/1
type: Range
persistent identity: range2138655
display id: range2138655
version: 1
top level: BBa_K542005
direction: direction/reverse
start: 1
end: 836
orientation: inline

BBa_K331035
type: SequenceAnnotation
persistent identity: annotation2138665
display id: annotation2138665
version: 1
top level: BBa_K542005
location: https://synbiohub.org/public/igem/BBa_K542005/annotation2138665/range2138665/1
component: BBa_K331035

https://synbiohub.org/public/igem/BBa_K542005/annotation2138665/range2138665/1
type: Range
persistent identity: range2138665
display id: range2138665
version: 1
top level: BBa_K542005
direction: direction/reverse
start: 845
end: 1755
orientation: inline

BBa_K331033
type: ComponentDefinition
persistent identity: BBa K331033
display id: BBa_K331033
version: 1
was generated by: iGEM to SBOL conversion
description: Tet Repressible C-terminal Arg Tagged ECFP (no terminator)
created: 2010-10-06T11:00:00Z
modified: 2015-05-08T01:12:05Z
mutable provenance: To come....
owned by: james
owned by: myers
mutable description: This is <partinfo>BBa_K331025</partinfo> with the tetracycline repressible promoter <partinfo>BBa_R0040</partinfo> added in front. This will be a component of a proof-of-concept part that will show localization of proteins into the interior of a microcompartment formed by the assembly of lumazine synthase proteins.
discontinued: false
dominant: false
experience: experience/Works
group u list: _463_
m user id: 0
owner id: 6831
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: To come..
star: false
creator: Adam Smith
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0034
component: BBa_R0040
component: BBa_K331002
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_K331002
sequence: https://synbiohub.org/public/igem/BBa_K331033_sequence/1

BBa_B0034
type: Component
persistent identity: component2083757
display id: component2083757
version: 1
top level: BBa_K331033
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component2083751
display id: component2083751
version: 1
top level: BBa_K331033
definition: p(tetR)
access: public

BBa_K331002
type: Component
persistent identity: component2083761
display id: component2083761
version: 1
top level: BBa_K331033
definition: BBa_K331002
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2083751
display id: annotation2083751
version: 1
top level: BBa_K331033
location: https://synbiohub.org/public/igem/BBa_K331033/annotation2083751/range2083751/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K331033/annotation2083751/range2083751/1
type: Range
persistent identity: range2083751
display id: range2083751
version: 1
top level: BBa_K331033
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2083757
display id: annotation2083757
version: 1
top level: BBa_K331033
location: https://synbiohub.org/public/igem/BBa_K331033/annotation2083757/range2083757/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K331033/annotation2083757/range2083757/1
type: Range
persistent identity: range2083757
display id: range2083757
version: 1
top level: BBa_K331033
direction: direction/reverse
start: 63
end: 74
orientation: inline

BBa_K331002
type: SequenceAnnotation
persistent identity: annotation2083761
display id: annotation2083761
version: 1
top level: BBa_K331033
location: https://synbiohub.org/public/igem/BBa_K331033/annotation2083761/range2083761/1
component: BBa_K331002

https://synbiohub.org/public/igem/BBa_K331033/annotation2083761/range2083761/1
type: Range
persistent identity: range2083761
display id: range2083761
version: 1
top level: BBa_K331033
direction: direction/reverse
start: 81
end: 836
orientation: inline

BBa_K331035
type: ComponentDefinition
persistent identity: BBa K331035
display id: BBa_K331035
version: 1
was generated by: iGEM to SBOL conversion
description: C-terminal Arg Tagged EYFP with transcriptional terminator
created: 2010-10-06T11:00:00Z
modified: 2015-05-08T01:12:05Z
mutable provenance: To come...
owned by: james
owned by: myers
mutable description: This is <partinfo>BBa_K331023</partinfo> with a transcriptional terminator <partinfo>BBa_B0015</partinfo> added behind. This will be a component of a proof-of-concept part that will show localization of proteins into the interior of a microcompartment formed by the assembly of lumazine synthase proteins.
discontinued: false
dominant: false
group u list: _463_
m user id: 0
owner id: 6831
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: To come...
star: false
creator: Adam Smith
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0010
component: BBa_K249006
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K249006
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K331035_sequence/1

BBa_B0010
type: Component
persistent identity: component2083774
display id: component2083774
version: 1
top level: BBa_K331035
definition: BBa_B0010
access: public

BBa_K249006
type: Component
persistent identity: component2083773
display id: component2083773
version: 1
top level: BBa_K331035
definition: BBa_K249006
access: public

BBa_B0012
type: Component
persistent identity: component2083776
display id: component2083776
version: 1
top level: BBa_K331035
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component2083772
display id: component2083772
version: 1
top level: BBa_K331035
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2083772
display id: annotation2083772
version: 1
top level: BBa_K331035
location: https://synbiohub.org/public/igem/BBa_K331035/annotation2083772/range2083772/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K331035/annotation2083772/range2083772/1
type: Range
persistent identity: range2083772
display id: range2083772
version: 1
top level: BBa_K331035
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K249006
type: SequenceAnnotation
persistent identity: annotation2083773
display id: annotation2083773
version: 1
top level: BBa_K331035
location: https://synbiohub.org/public/igem/BBa_K331035/annotation2083773/range2083773/1
component: BBa_K249006

https://synbiohub.org/public/igem/BBa_K331035/annotation2083773/range2083773/1
type: Range
persistent identity: range2083773
display id: range2083773
version: 1
top level: BBa_K331035
direction: direction/reverse
start: 19
end: 774
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2083774
display id: annotation2083774
version: 1
top level: BBa_K331035
location: https://synbiohub.org/public/igem/BBa_K331035/annotation2083774/range2083774/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K331035/annotation2083774/range2083774/1
type: Range
persistent identity: range2083774
display id: range2083774
version: 1
top level: BBa_K331035
direction: direction/forward
start: 783
end: 862
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2083776
display id: annotation2083776
version: 1
top level: BBa_K331035
location: https://synbiohub.org/public/igem/BBa_K331035/annotation2083776/range2083776/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K331035/annotation2083776/range2083776/1
type: Range
persistent identity: range2083776
display id: range2083776
version: 1
top level: BBa_K331035
direction: direction/forward
start: 871
end: 911
orientation: inline

BBa_K542001
type: ComponentDefinition
persistent identity: BBa K542001
display id: BBa_K542001
version: 1
was generated by: iGEM to SBOL conversion
description: pLacI Regulated Lumazine Synthase
created: 2011-09-21T11:00:00Z
modified: 2015-05-08T01:12:38Z
mutable provenance: [http://partsregistry.org/Part:BBa_J04500 BBa_J04500] obtained from iGEM distribution plate and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K542000 BBa_K542000] was assembled by Lethbridge 2011 team.
owned by: james
owned by: myers
mutable description: Made by assembling [http://partsregistry.org/Part:BBa_J04500 BBa_J04500] with [http://partsregistry.org/wiki/index.php?title=Part:BBa_K542000 BBa_K542000] IPTG inducible Lumazine Synthase. Intermediate part to assemble the "Co-localization Construct".
discontinued: false
dominant: false
experience: experience/Works
group u list: _709_
m user id: 0
owner id: 6015
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Intermediate part to assemble the "Co-localization Construct".
star: false
creator: Anthony Vuong
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_J04500
component: BBa_K542000
sequence annotation: BBa_J04500
sequence annotation: BBa_K542000
sequence: https://synbiohub.org/public/igem/BBa_K542001_sequence/1

BBa_J04500
type: Component
persistent identity: component2137925
display id: component2137925
version: 1
top level: BBa_K542001
definition: BBa_J04500
access: public

BBa_K542000
type: Component
persistent identity: component2137934
display id: component2137934
version: 1
top level: BBa_K542001
definition: BBa_K542000
access: public

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2137925
display id: annotation2137925
version: 1
top level: BBa_K542001
location: https://synbiohub.org/public/igem/BBa_K542001/annotation2137925/range2137925/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K542001/annotation2137925/range2137925/1
type: Range
persistent identity: range2137925
display id: range2137925
version: 1
top level: BBa_K542001
direction: direction/reverse
start: 1
end: 220
orientation: inline

BBa_K542000
type: SequenceAnnotation
persistent identity: annotation2137934
display id: annotation2137934
version: 1
top level: BBa_K542001
location: https://synbiohub.org/public/igem/BBa_K542001/annotation2137934/range2137934/1
component: BBa_K542000

https://synbiohub.org/public/igem/BBa_K542001/annotation2137934/range2137934/1
type: Range
persistent identity: range2137934
display id: range2137934
version: 1
top level: BBa_K542001
direction: direction/reverse
start: 227
end: 828
orientation: inline

BBa_K542003
type: ComponentDefinition
persistent identity: BBa K542003
display id: BBa_K542003
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD Regulated TetR
created: 2011-09-22T11:00:00Z
modified: 2015-05-08T01:12:38Z
mutable provenance: [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13453 BBa_I13453] and [http://partsregistry.org/Part:BBa_P0440 BBa_P0440] were both obtained from iGEM distribution plates.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Made by assembling [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13453 BBa_I13453] with [http://partsregistry.org/Part:BBa_P0440 BBa_P0440]. Production of TetR is regulated by the pBAD promoter([http://partsregistry.org/Part:BBa_I13453 BBa_I13453]). TetR is an inhibitor of the constitutively "on" promoter pTet--[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0040 BBa_R0040]). Therefore, this part may be used in conjunction with the pTet promoter as an "inverter"; pTet will be "turned off" in the presence of arabinose. Arabinose -> pBAD "on" -> TetR -> pTet "off" Intermediate part to assemble the "Co-localization Construct".
discontinued: false
dominant: false
experience: experience/Works
group u list: _709_
m user id: 0
owner id: 6015
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Intermediate part to assemble the "Co-localization Construct".
star: false
creator: Anthony Vuong
type: DnaRegion
role: partType/Inverter
role: SO:0000804
component: BBa_P0440
component: BBa_I13453
sequence annotation: BBa_I13453
sequence annotation: BBa_P0440
sequence: https://synbiohub.org/public/igem/BBa_K542003_sequence/1

BBa_P0440
type: Component
persistent identity: component2138601
display id: component2138601
version: 1
top level: BBa_K542003
definition: BBa_P0440
access: public

BBa_I13453
type: Component
persistent identity: component2138588
display id: component2138588
version: 1
top level: BBa_K542003
definition: BBa_I13453
access: public

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2138588
display id: annotation2138588
version: 1
top level: BBa_K542003
location: https://synbiohub.org/public/igem/BBa_K542003/annotation2138588/range2138588/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K542003/annotation2138588/range2138588/1
type: Range
persistent identity: range2138588
display id: range2138588
version: 1
top level: BBa_K542003
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_P0440
type: SequenceAnnotation
persistent identity: annotation2138601
display id: annotation2138601
version: 1
top level: BBa_K542003
location: https://synbiohub.org/public/igem/BBa_K542003/annotation2138601/range2138601/1
component: BBa_P0440

https://synbiohub.org/public/igem/BBa_K542003/annotation2138601/range2138601/1
type: Range
persistent identity: range2138601
display id: range2138601
version: 1
top level: BBa_K542003
direction: direction/reverse
start: 139
end: 978
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_K249006
type: ComponentDefinition
persistent identity: BBa K249006
display id: BBa_K249006
version: 1
was generated by: iGEM to SBOL conversion
description: Fusion EYFP with C-terminal Arginine Tag
created: 2009-08-25T11:00:00Z
modified: 2015-05-08T01:11:40Z
mutable provenance: BioBrick Foundation EYFP fused with a synthesized Arginine tag
owned by: james
owned by: myers
mutable description: This Yellow Fluorescent protein has been fused with 10 C-terminal Arginines to target it into the Lumazine-based microcompartment.
discontinued: false
dominant: false
group u list: _342_
m user id: 0
owner id: 3171
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Standard
star: false
creator: Roxanne Shank
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence: https://synbiohub.org/public/igem/BBa_K249006_sequence/1

BBa_K331002
type: ComponentDefinition
persistent identity: BBa K331002
display id: BBa_K331002
version: 1
was generated by: iGEM to SBOL conversion
description: ECFP with C-terminal Arginine Tag
created: 2010-06-27T11:00:00Z
modified: 2015-05-08T01:12:04Z
mutable provenance: The idea came from: Seebeck, F. P., Woycechowsky, K. J., Zhuang, W., Rabe, J. P., and Hilvert, D., (2006). A simple tagging system for protein encapulation. J. Am. Chem. Soc. 128, 4516-4517
owned by: james
owned by: myers
mutable description: This protein is the ECFP fused with the C-terminal Arginine tag found in BBa_K249005. It is designed to be part of a construct for targeting into the lumazine synthase microcompatment (BBa_K331000)
discontinued: false
dominant: false
experience: experience/None
group u list: _463_
m user id: 0
owner id: 4444
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Lisza Bruder
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: start
sequence annotation: Arginine Tag
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K331002_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2071685
display id: annotation2071685
version: 1
top level: BBa_K331002
location: https://synbiohub.org/public/igem/BBa_K331002/annotation2071685/range2071685/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K331002/annotation2071685/range2071685/1
type: Range
persistent identity: range2071685
display id: range2071685
version: 1
top level: BBa_K331002
direction: direction/forward
start: 1
end: 3
orientation: inline

Arginine Tag
type: SequenceAnnotation
persistent identity: annotation2071687
display id: annotation2071687
version: 1
top level: BBa_K331002
location: https://synbiohub.org/public/igem/BBa_K331002/annotation2071687/range2071687/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K331002/annotation2071687/range2071687/1
type: Range
persistent identity: range2071687
display id: range2071687
version: 1
top level: BBa_K331002
direction: direction/forward
start: 725
end: 753
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2071686
display id: annotation2071686
version: 1
top level: BBa_K331002
location: https://synbiohub.org/public/igem/BBa_K331002/annotation2071686/range2071686/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K331002/annotation2071686/range2071686/1
type: Range
persistent identity: range2071686
display id: range2071686
version: 1
top level: BBa_K331002
direction: direction/forward
start: 754
end: 756
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_K542000
type: ComponentDefinition
persistent identity: BBa K542000
display id: BBa_K542000
version: 1
was generated by: iGEM to SBOL conversion
description: Lumazine Synthase with Transciptional Terminator (No Promoter)
created: 2011-09-21T11:00:00Z
modified: 2015-05-08T01:12:38Z
mutable provenance: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K249002 BBa_K249002] and [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] were obtained from iGEM distribution plates.
owned by: james
owned by: myers
mutable description: Composite part: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K249002 BBa_K249002] + [http://partsregistry.org/wiki/index.php/Part:BBa_B0010 BBa_B0010] + [http://partsregistry.org/wiki/index.php/Part:BBa_B0010 BBa_B0010] + [http://partsregistry.org/wiki/index.php/Part:BBa_B0012 BBa_B0012] Made by assembling [http://partsregistry.org/wiki/index.php?title=Part:BBa_K249002 BBa_K249002] with [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] Since this part is lacking the promoter, Lumazine Synthase production may be regulated by the addition different promoters upstream. Regulation of Lumazine Synthase will be dependent on the promoter being utilized.
discontinued: false
dominant: false
experience: experience/Works
group u list: _709_
m user id: 0
owner id: 6015
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Intermediate part to assemble the "Co-localization Construct".
star: false
creator: Anthony Vuong
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0015
component: BBa_K249002
sequence annotation: BBa_K249002
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K542000_sequence/1

BBa_B0015
type: Component
persistent identity: component2137880
display id: component2137880
version: 1
top level: BBa_K542000
definition: BBa_B0015
access: public

BBa_K249002
type: Component
persistent identity: component2137873
display id: component2137873
version: 1
top level: BBa_K542000
definition: BBa_K249002
access: public

BBa_K249002
type: SequenceAnnotation
persistent identity: annotation2137873
display id: annotation2137873
version: 1
top level: BBa_K542000
location: https://synbiohub.org/public/igem/BBa_K542000/annotation2137873/range2137873/1
component: BBa_K249002

https://synbiohub.org/public/igem/BBa_K542000/annotation2137873/range2137873/1
type: Range
persistent identity: range2137873
display id: range2137873
version: 1
top level: BBa_K542000
direction: direction/reverse
start: 1
end: 465
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2137880
display id: annotation2137880
version: 1
top level: BBa_K542000
location: https://synbiohub.org/public/igem/BBa_K542000/annotation2137880/range2137880/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K542000/annotation2137880/range2137880/1
type: Range
persistent identity: range2137880
display id: range2137880
version: 1
top level: BBa_K542000
direction: direction/reverse
start: 474
end: 602
orientation: inline

BBa_J04500
type: ComponentDefinition
persistent identity: BBa J04500
display id: BBa_J04500
version: 1
was generated by: iGEM to SBOL conversion
description: IPTG inducible promoter with RBS
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: R0010.B0034
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 326
owning group id: 16
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Kristen DeCelle
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J04500_sequence/1

BBa_B0034
type: Component
persistent identity: component1508159
display id: component1508159
version: 1
top level: BBa_J04500
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component1508149
display id: component1508149
version: 1
top level: BBa_J04500
definition: LacI
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1508149
display id: annotation1508149
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
type: Range
persistent identity: range1508149
display id: range1508149
version: 1
top level: BBa_J04500
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1508159
display id: annotation1508159
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
type: Range
persistent identity: range1508159
display id: range1508159
version: 1
top level: BBa_J04500
direction: direction/forward
start: 209
end: 220
orientation: inline

BBa_P0440
type: ComponentDefinition
persistent identity: BBa P0440
display id: BBa_P0440
version: 1
was generated by: iGEM to SBOL conversion
description: PoPS -> TetR [S0151]
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:10Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0040
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_P0440_sequence/1

BBa_C0040
type: Component
persistent identity: component944896
display id: component944896
version: 1
top level: BBa_P0440
definition: tetR
access: public

BBa_B0034
type: Component
persistent identity: component944886
display id: component944886
version: 1
top level: BBa_P0440
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component944912
display id: component944912
version: 1
top level: BBa_P0440
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component944902
display id: component944902
version: 1
top level: BBa_P0440
definition: BBa_B0010
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation944886
display id: annotation944886
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944886/range944886/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_P0440/annotation944886/range944886/1
type: Range
persistent identity: range944886
display id: range944886
version: 1
top level: BBa_P0440
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation944896
display id: annotation944896
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944896/range944896/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_P0440/annotation944896/range944896/1
type: Range
persistent identity: range944896
display id: range944896
version: 1
top level: BBa_P0440
direction: direction/forward
start: 19
end: 678
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944902
display id: annotation944902
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944902/range944902/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0440/annotation944902/range944902/1
type: Range
persistent identity: range944902
display id: range944902
version: 1
top level: BBa_P0440
direction: direction/forward
start: 712
end: 791
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944912
display id: annotation944912
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944912/range944912/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0440/annotation944912/range944912/1
type: Range
persistent identity: range944912
display id: range944912
version: 1
top level: BBa_P0440
direction: direction/forward
start: 800
end: 840
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

BBa_K249002
type: ComponentDefinition
persistent identity: BBa K249002
display id: BBa_K249002
version: 1
was generated by: iGEM to SBOL conversion
description: Lumazine Synthase
created: 2009-08-25T11:00:00Z
modified: 2015-05-08T01:11:40Z
mutable provenance: A Science Paper?
owned by: james
owned by: myers
mutable description: Lumazine Synthase is an enzyme which creates Lumazine, a product which aggregates forming a hollow spheroid which can act as a mirocompartment, or artificial organelle. The Lumazine forms negatively charged pores, which can be used to introduce proteins. The proteins which are being introduced into the microcompartment must be equipped with an Arginine Tag.
discontinued: false
dominant: false
experience: experience/None
group u list: _242_342_
m user id: 0
owner id: 3171
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: point mutations of EcoRI and PstI sites?
star: false
creator: Roxanne Shank
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K249002_sequence/1

https://synbiohub.org/public/igem/BBa_K542008_sequence/1
type: Sequence
persistent identity: BBa K542008 sequence
display id: BBa_K542008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgcagatttatgaaggcaaactgaccgcggaaggcctgcgctttggcattgtggcgagccgctttaaccatgcgctggtggatcgcctggtggaaggcgcgattgattgcattgtgcgccatggtggtcgcgaagaagatattaccctggtgcgcgtgccgggcagctgggaaattccggtggcggcgggcgaactggcgcgcaaagaagatattgatgcggtgattgcgattggcgtgctgattgaaggcgcggaaccgcattttgattatattgcgagcgaagtgagcaaaggcctggcgaacctgagcctggaactgcgcaaaccgattacctttggcgtgattaccgcggatgaactggaagaagcgattgaacgcgcgggcaccaaacatggcaacaaaggctgggaagcggcgctgagcgcgattgaaatggcgaacctgtttaaaagcctgcgctagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K542004_sequence/1
type: Sequence
persistent identity: BBa K542004 sequence
display id: BBa_K542004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgcagatttatgaaggcaaactgaccgcggaaggcctgcgctttggcattgtggcgagccgctttaaccatgcgctggtggatcgcctggtggaaggcgcgattgattgcattgtgcgccatggtggtcgcgaagaagatattaccctggtgcgcgtgccgggcagctgggaaattccggtggcggcgggcgaactggcgcgcaaagaagatattgatgcggtgattgcgattggcgtgctgattgaaggcgcggaaccgcattttgattatattgcgagcgaagtgagcaaaggcctggcgaacctgagcctggaactgcgcaaaccgattacctttggcgtgattaccgcggatgaactggaagaagcgattgaacgcgcgggcaccaaacatggcaacaaaggctgggaagcggcgctgagcgcgattgaaatggcgaacctgtttaaaagcctgcgctagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K542005_sequence/1
type: Sequence
persistent identity: BBa K542005 sequence
display id: BBa_K542005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagcactagagccgccgccgccgccgccgccgccgccgctaatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagcactagagccgccgccgccgccgccgccgccgccgctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K331033_sequence/1
type: Sequence
persistent identity: BBa K331033 sequence
display id: BBa_K331033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagcactagagccgccgccgccgccgccgccgccgccgctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K331035_sequence/1
type: Sequence
persistent identity: BBa K331035 sequence
display id: BBa_K331035_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagcactagagccgccgccgccgccgccgccgccgccgctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K542001_sequence/1
type: Sequence
persistent identity: BBa K542001 sequence
display id: BBa_K542001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgcagatttatgaaggcaaactgaccgcggaaggcctgcgctttggcattgtggcgagccgctttaaccatgcgctggtggatcgcctggtggaaggcgcgattgattgcattgtgcgccatggtggtcgcgaagaagatattaccctggtgcgcgtgccgggcagctgggaaattccggtggcggcgggcgaactggcgcgcaaagaagatattgatgcggtgattgcgattggcgtgctgattgaaggcgcggaaccgcattttgattatattgcgagcgaagtgagcaaaggcctggcgaacctgagcctggaactgcgcaaaccgattacctttggcgtgattaccgcggatgaactggaagaagcgattgaacgcgcgggcaccaaacatggcaacaaaggctgggaagcggcgctgagcgcgattgaaatggcgaacctgtttaaaagcctgcgctagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K542003_sequence/1
type: Sequence
persistent identity: BBa K542003 sequence
display id: BBa_K542003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K249006_sequence/1
type: Sequence
persistent identity: BBa K249006 sequence
display id: BBa_K249006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagcactagagccgccgccgccgccgccgccgccgccgctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K331002_sequence/1
type: Sequence
persistent identity: BBa K331002 sequence
display id: BBa_K331002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagcactagagccgccgccgccgccgccgccgccgccgctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K542000_sequence/1
type: Sequence
persistent identity: BBa K542000 sequence
display id: BBa_K542000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcagatttatgaaggcaaactgaccgcggaaggcctgcgctttggcattgtggcgagccgctttaaccatgcgctggtggatcgcctggtggaaggcgcgattgattgcattgtgcgccatggtggtcgcgaagaagatattaccctggtgcgcgtgccgggcagctgggaaattccggtggcggcgggcgaactggcgcgcaaagaagatattgatgcggtgattgcgattggcgtgctgattgaaggcgcggaaccgcattttgattatattgcgagcgaagtgagcaaaggcctggcgaacctgagcctggaactgcgcaaaccgattacctttggcgtgattaccgcggatgaactggaagaagcgattgaacgcgcgggcaccaaacatggcaacaaaggctgggaagcggcgctgagcgcgattgaaatggcgaacctgtttaaaagcctgcgctagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J04500_sequence/1
type: Sequence
persistent identity: BBa J04500 sequence
display id: BBa_J04500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P0440_sequence/1
type: Sequence
persistent identity: BBa P0440 sequence
display id: BBa_P0440_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K249002_sequence/1
type: Sequence
persistent identity: BBa K249002 sequence
display id: BBa_K249002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcagatttatgaaggcaaactgaccgcggaaggcctgcgctttggcattgtggcgagccgctttaaccatgcgctggtggatcgcctggtggaaggcgcgattgattgcattgtgcgccatggtggtcgcgaagaagatattaccctggtgcgcgtgccgggcagctgggaaattccggtggcggcgggcgaactggcgcgcaaagaagatattgatgcggtgattgcgattggcgtgctgattgaaggcgcggaaccgcattttgattatattgcgagcgaagtgagcaaaggcctggcgaacctgagcctggaactgcgcaaaccgattacctttggcgtgattaccgcggatgaactggaagaagcgattgaacgcgcgggcaccaaacatggcaacaaaggctgggaagcggcgctgagcgcgattgaaatggcgaacctgtttaaaagcctgcgctag
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z