LOCUS       BBa_K542009             3346 bp    DNA     linear       05-Nov-2024
DEFINITION  pLacI Regulated AG43
ACCESSION   BBa_K542009
VERSION     BBa_K542009.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..3346
                     /label=agn43
     CDS             227..3346
                     /label=BBa_K346007
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aacgacatct
      241 gaatacctgc tacaggctgg tatggaatca catgacgggc gctttcgtgg ttgcctccga
      301 actggcccgc gcacggggta aacgtggcgg tgtggcggtt gcactgtctc ttgccgcagt
      361 cacgtcactc ccggtgctgg ctgctgacat cgttgtgcac ccgggagaaa ccgtgaacgg
      421 cggaacactg gcaaatcatg acaaccagat tgtcttcggt acgaccaacg gaatgaccat
      481 cagtaccggg ctggagtatg ggccggataa cgaggccaat accggcgggc aatgggtaca
      541 ggatggcgga acagccaaca aaacgactgt caccagtggt ggtcttcaga gagtgaaccc
      601 cggtggaagt gtctcagaca cggttatcag tgccggaggc ggacagagcc ttcagggacg
      661 ggctgtgaac accacgctga atggtggcga acagtggatg catgaggggg cgatagccac
      721 aggaaccgtc attaatgata agggctggca ggtcgtcaag cccggtacag tggcaacgga
      781 taccgttgtt aataccgggg cggaaggggg accggatgca gaaaacggtg ataccgggca
      841 gtttgttcgc ggggatgccg tacgcacaac catcaataaa aacggtcgcc agattgtgag
      901 agctgaagga acggcaaata ccactgtggt ttatgccggc ggcgaccaga ctgtacatgg
      961 tcacgcactg gataccacgc tgaatggggg ataccagtat gtgcacaacg gcggtacagc
     1021 gtctgacact gttgtgaaca gtgacggctg gcagattgtc aaaaacgggg gtgtggccgg
     1081 gaataccacc gttaatcaga agggcagact gcaagtggac gccggtggta cagccacgaa
     1141 tgtcaccctg aagcagggcg gcgcactggt taccagtacg gctgcaaccg ttaccggcat
     1201 aaaccgcctg ggagcattct ctgttgtgga gggtaaagct gataatgtcg tactggaaaa
     1261 tggcggacgc ctggatgtgc tgaccggaca cacagccact aatacccgcg tggatgatgg
     1321 cggaacgctg gatgtccgca acggtggcac cgccaccacc gtatccatgg gaaatggcgg
     1381 tgtactgctg gccgattccg gtgccgctgt cagtggtacc cggagcgacg gaaaggcatt
     1441 cagtatcgga ggcggtcagg cggatgccct gatgctggaa aaaggcagtt cattcacgct
     1501 gaacgccggt gatacggcca cggataccac ggtaaatggc ggactgttca ccgccagggg
     1561 cggcacactg gcgggcacca ccacgctgaa taacggcgcc atacttaccc tttccgggaa
     1621 gacggtgaac aacgataccc tgaccatccg tgaaggcgat gcactcctgc aaggaggctc
     1681 tctcaccggt aacggcagcg tggaaaaatc aggaagtggc acactcactg tcagcaacac
     1741 cacactcacc cagaaagccg tcaacctgaa tgaaggcacg ctgacgctga acgacagtac
     1801 cgtcaccacg gatgtcattg ctcagcgcgg tacagccctg aagctgaccg gcagcactgt
     1861 gctgaacggt gccattgacc ccacgaatgt cactctcgcc tccggtgcca cctggaatat
     1921 ccccgataac gccacggtgc agtcggtggt ggatgacctc agccatgccg gacagattca
     1981 tttcacctcc acccgcacag ggaagttcgt accggcaacc ctgaaagtga aaaacctgaa
     2041 cggacagaat ggcaccatca gcctgcgtgt acgcccggat atggcacaga acaatgctga
     2101 cagactggtc attgacggcg gcagggcaac cggaaaaacc atcctgaacc tggtgaacgc
     2161 cggcaacagt gcgtcggggc tggcgaccag cggtaagggt attcaggtgg tggaagccat
     2221 taacggtgcc accacggagg aaggggcctt tgtccagggg aacaggctgc aagccggtgc
     2281 ctttaactac tccctcaacc gggacagtga tgagagctgg tatctgcgca gtgaaaatgc
     2341 ttatcgtgca gaagtccccc tgtatgcctc catgctgaca caggcaatgg actatgaccg
     2401 gattgtggca ggctcccgca gccatcagac cggtgtaaat ggtgaaaaca acagcgtccg
     2461 tctcagcatt cagggcggtc atctcggtca cgataacaat ggcggtattg cccgtggggc
     2521 cacgccggaa agcagcggca gctatggatt cgtccgtctg gagggtgacc tgatgagaac
     2581 agaggttgcc ggtatgtctg tgaccgcggg ggtatatggt gctgctggcc attcttccgt
     2641 tgatgttaag gatgatgacg gctcccgtgc cggcacggtc cgggatgatg ccggcagcct
     2701 gggcggatac ctgaatctgg tacacacgtc ctccggcctg tgggctgaca ttgtggcaca
     2761 gggaacccgc cacagcatga aagcgtcatc ggacaataac gacttccgcg cccggggctg
     2821 gggctggctg ggctcactgg aaaccggtct gcccttcagt atcactgaca acctgatgct
     2881 ggagccacaa ctgcaatata cctggcaggg actttccctg gatgacggta aggacaacgc
     2941 cggttatgtg aagttcgggc atggcagtgc acaacatgtg cgtgccggtt tccgtctggg
     3001 cagccacaac gatatgacct ttggcgaagg cacctcatcc cgtgcccccc tgcgtgacag
     3061 tgcaaaacac agtgtgagtg aattaccggt gaactggtgg gtacagcctt ctgttatccg
     3121 caccttcagc tcccggggag atatgcgtgt ggggacttcc actgcaagca gcgggatgac
     3181 gttctctccc tcacagaatg gcacatcact ggacctgcaa gccggactgg aagcccgtgt
     3241 ccgggaaaat atcaccctgg gcgttcaggc cggttatgcc cacagcgtca gcggcagcag
     3301 cgctgaaggg tataacggtc aggccacact gaatgtgacc ttctga
//