BBa_K542011 1 BBa_K542011 Catechol 2,3-dioxygenase with C-term Arg-tag (xylE-Arg) 2011-09-27T11:00:00Z 2015-05-08T01:12:39Z The xylE gene was PCR'd out of genomic DNA provided by David Lain Houston* with primers adding [http://openwetware.org/wiki/The_BioBricks_Foundation:Standards/Technical/Formats#BBF_RFC_23_.28aka_Biofusion.2C_Silver_lab.29 Silver Lab Biofusion Standard] prefix/suffix. A C-terminal arginine tail was then added ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K249005 BBa_K249005]). *David Lain Houston, PhD Student Institute of Cellular, Molecular, and Systems Biology Susan Rosser Group Arnott Lab University of Glasgow The xylE gene was PCR'd out of genomic DNA provided by David Lain Houston* with primers adding [http://openwetware.org/wiki/The_BioBricks_Foundation:Standards/Technical/Formats#BBF_RFC_23_.28aka_Biofusion.2C_Silver_lab.29 Silver Lab Biofusion Standard] prefix/suffix. A C-terminal arginine tail was then added ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K249005 BBa_K249005]). Based on [http://2010.igem.org/Team:Lethbridge/Results#Results_2 characterization on Arg-tagged fluorescent proteins] done by the Lethbridge 2010 team, it was decided that the Arg-tag on xylE would be C-terminal. *David Lain Houston, PhD Student Institute of Cellular, Molecular, and Systems Biology Susan Rosser Group Arnott Lab University of Glasgow false false _709_ 0 6015 9 Not in stock false Based on [http://2010.igem.org/Team:Lethbridge/Results#Results_2 characterization on Arg-tagged fluorescent proteins] done by the Lethbridge 2010 team, it was decided that the Arg-tag on xylE would be C-terminal. false Anthony Vuong BBa_K542011_sequence 1 atgaacaaaggtgtaatgcgaccgggccatgtgcagctgcgtgtactggacatgagcaaggccctggaacactacgtcgagttgctgggcctgatcgagatggaccgtgacgaccagggccgtgtctatctgaaggcttggaccgaagtggataagttttccctggtgctacgcgaggctgacgagccgggcatggattttatgggtttcaaggttgtggatgaggatgctctccggcaactggagcgggatctgatggcatatggctgtgccgttgagcagctacccgcaggtgaactgaacagttgtggccggcgcgtgcgcttccaggccccctccgggcatcacttcgagttgtatgcagacaaggaatatactggaaagtggggtttgaatgacgtcaatcccgaggcatggccgcgcgatctgaaaggtatggcggctgtgcgtttcgaccacgccctcatgtatggcgacgaattgccggcgacctatgacctgttcaccaaggtgctcggtttctatctggccgaacaggtgctggacgaaaatggcacgcgcgtcgcccagtttctcagtctgtcgaccaaggcccacgacgtggccttcattcaccatccggaaaaaggccgcctccatcatgtgtccttccacctcgaaacctgggaagacttgcttcgcgccgccgacctgatctccatgaccgacacatctatcgatatcggcccaacccgccacggcctcactcacggcaagaccatctacttcttcgacccgtccggtaaccgcaacgaagtgttctgcgggggagattacaactacccggaccacaaaccggtgacctggaccaccgaccagctgggcaaggcgatcttttaccacgaccgcattctcaacgaacgattcatgaccgtgctgaccactagacgccgccgccgccgccgccgccgccgccgctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z