BBa_K542012 1 BBa_K542012 Catechol 2,3-dioxygenase with a C-terminal Arg-tag and Double Terminator (no promoter/RBS) 2011-09-27T11:00:00Z 2015-05-08T01:12:39Z ([http://partsregistry.org/Part:BBa_K542011 BBa_K542011]) was assembled by the Lethbridge 2011 team and ([http://partsregistry.org/Part:BBa_B0015 BBa_B0015]) was obtained from iGEM Distribution plates. Made by assembling ([http://partsregistry.org/Part:BBa_K542011 BBa_K542011]) with ([http://partsregistry.org/Part:BBa_B0015 BBa_B0015]). Arginine tag intended to allow for targeting into Lumazine Synthase microcompartment ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K249002 BBa_K249002]). Eventually plan to produce a construct with the [http://partsregistry.org/wiki/index.php?title=Part:BBa_K542004 microcompartment portion] of the "Co-localizing Construct". false false _709_ 0 6015 9 Not in stock false Arginine tag intended to allow for targeting into Lumazine Synthase microcompartment ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K249002 BBa_K249002]). false Anthony Vuong component2145535 1 BBa_B0015 component2145528 1 BBa_K542011 annotation2145535 1 BBa_B0015 range2145535 1 969 1097 annotation2145528 1 BBa_K542011 range2145528 1 1 960 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K542011 1 BBa_K542011 Catechol 2,3-dioxygenase with C-term Arg-tag (xylE-Arg) 2011-09-27T11:00:00Z 2015-05-08T01:12:39Z The xylE gene was PCR'd out of genomic DNA provided by David Lain Houston* with primers adding [http://openwetware.org/wiki/The_BioBricks_Foundation:Standards/Technical/Formats#BBF_RFC_23_.28aka_Biofusion.2C_Silver_lab.29 Silver Lab Biofusion Standard] prefix/suffix. A C-terminal arginine tail was then added ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K249005 BBa_K249005]). *David Lain Houston, PhD Student Institute of Cellular, Molecular, and Systems Biology Susan Rosser Group Arnott Lab University of Glasgow The xylE gene was PCR'd out of genomic DNA provided by David Lain Houston* with primers adding [http://openwetware.org/wiki/The_BioBricks_Foundation:Standards/Technical/Formats#BBF_RFC_23_.28aka_Biofusion.2C_Silver_lab.29 Silver Lab Biofusion Standard] prefix/suffix. A C-terminal arginine tail was then added ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K249005 BBa_K249005]). Based on [http://2010.igem.org/Team:Lethbridge/Results#Results_2 characterization on Arg-tagged fluorescent proteins] done by the Lethbridge 2010 team, it was decided that the Arg-tag on xylE would be C-terminal. *David Lain Houston, PhD Student Institute of Cellular, Molecular, and Systems Biology Susan Rosser Group Arnott Lab University of Glasgow false false _709_ 0 6015 9 Not in stock false Based on [http://2010.igem.org/Team:Lethbridge/Results#Results_2 characterization on Arg-tagged fluorescent proteins] done by the Lethbridge 2010 team, it was decided that the Arg-tag on xylE would be C-terminal. false Anthony Vuong BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K542012_sequence 1 atgaacaaaggtgtaatgcgaccgggccatgtgcagctgcgtgtactggacatgagcaaggccctggaacactacgtcgagttgctgggcctgatcgagatggaccgtgacgaccagggccgtgtctatctgaaggcttggaccgaagtggataagttttccctggtgctacgcgaggctgacgagccgggcatggattttatgggtttcaaggttgtggatgaggatgctctccggcaactggagcgggatctgatggcatatggctgtgccgttgagcagctacccgcaggtgaactgaacagttgtggccggcgcgtgcgcttccaggccccctccgggcatcacttcgagttgtatgcagacaaggaatatactggaaagtggggtttgaatgacgtcaatcccgaggcatggccgcgcgatctgaaaggtatggcggctgtgcgtttcgaccacgccctcatgtatggcgacgaattgccggcgacctatgacctgttcaccaaggtgctcggtttctatctggccgaacaggtgctggacgaaaatggcacgcgcgtcgcccagtttctcagtctgtcgaccaaggcccacgacgtggccttcattcaccatccggaaaaaggccgcctccatcatgtgtccttccacctcgaaacctgggaagacttgcttcgcgccgccgacctgatctccatgaccgacacatctatcgatatcggcccaacccgccacggcctcactcacggcaagaccatctacttcttcgacccgtccggtaaccgcaacgaagtgttctgcgggggagattacaactacccggaccacaaaccggtgacctggaccaccgaccagctgggcaaggcgatcttttaccacgaccgcattctcaacgaacgattcatgaccgtgctgaccactagacgccgccgccgccgccgccgccgccgccgctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K542011_sequence 1 atgaacaaaggtgtaatgcgaccgggccatgtgcagctgcgtgtactggacatgagcaaggccctggaacactacgtcgagttgctgggcctgatcgagatggaccgtgacgaccagggccgtgtctatctgaaggcttggaccgaagtggataagttttccctggtgctacgcgaggctgacgagccgggcatggattttatgggtttcaaggttgtggatgaggatgctctccggcaactggagcgggatctgatggcatatggctgtgccgttgagcagctacccgcaggtgaactgaacagttgtggccggcgcgtgcgcttccaggccccctccgggcatcacttcgagttgtatgcagacaaggaatatactggaaagtggggtttgaatgacgtcaatcccgaggcatggccgcgcgatctgaaaggtatggcggctgtgcgtttcgaccacgccctcatgtatggcgacgaattgccggcgacctatgacctgttcaccaaggtgctcggtttctatctggccgaacaggtgctggacgaaaatggcacgcgcgtcgcccagtttctcagtctgtcgaccaaggcccacgacgtggccttcattcaccatccggaaaaaggccgcctccatcatgtgtccttccacctcgaaacctgggaagacttgcttcgcgccgccgacctgatctccatgaccgacacatctatcgatatcggcccaacccgccacggcctcactcacggcaagaccatctacttcttcgacccgtccggtaaccgcaacgaagtgttctgcgggggagattacaactacccggaccacaaaccggtgacctggaccaccgaccagctgggcaaggcgatcttttaccacgaccgcattctcaacgaacgattcatgaccgtgctgaccactagacgccgccgccgccgccgccgccgccgccgctaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z