LOCUS       BBa_K543003              615 bp    DNA     linear       25-Nov-2024
DEFINITION  HNS mutant T108I protein generator
ACCESSION   BBa_K543003
VERSION     BBa_K543003.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     misc_feature    65..478
                     /label=HNS protein
     misc_feature    387..387
                     /label=mutation T108I
     CDS             65..478
                     /label=BBa_K543002
     stem_loop       498..541
                     /label=stem_loop
     misc_feature    487..566
                     /label=BBa_B0010
     terminator      487..566
                     /label=BBa_B0010
     stem_loop       582..601
                     /label=T7 TE
     misc_feature    608..608
                     /label=stop
     polyA_site      602..615
                     /label=polya
     misc_feature    575..615
                     /label=BBa_B0012
     terminator      575..615
                     /label=BBa_B0012
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagattaaag aggagaaata
       61 ctagatgagc gaagcactta aaattctgaa caacatccgt actcttcgtg cgcaggcaag
      121 agaatgtaca cttgaaacgc tggaagaaat gctggaaaaa ttagaagttg ttgttaacga
      181 acgtcgcgaa gaagaaagcg cggctgctgc tgaagttgaa gagcgcactc gtaaactgca
      241 gcaatatcgc gaaatgctga tcgctgacgg tattgacccg aacgaactgc tgaatagcct
      301 tgctgccgtt aaatctggca ccaaagctaa acgtgctcag cgtccggcaa aatatagcta
      361 cgttgacgaa aacggcgaaa ctaaaatctg gactggccaa ggccgtactc cagctgtaat
      421 caaaaaagca atggatgagc aaggtaaatc cctcgacgat ttcctgatca agcaataata
      481 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
      541 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
      601 ctttctgcgt ttata
//