LOCUS       BBa_K543010             1918 bp    DNA     linear       22-Nov-2024
DEFINITION  AHL inducible CheZ generator
ACCESSION   BBa_K543010
VERSION     BBa_K543010.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..63
                     /label=prefix
     misc_feature    235..235
                     /label=T
     misc_feature    553..553
                     /label=A
     CDS             62..811
                     /label=luxR
     misc_feature    62..817
                     /label=BBa_C0062
     misc_feature    818..842
                     /label=Help:Barcodes
     CDS             62..817
                     /label=BBa_C0062
     stem_loop       862..905
                     /label=stem_loop
     misc_feature    851..930
                     /label=BBa_B0010
     terminator      851..930
                     /label=BBa_B0010
     stem_loop       946..965
                     /label=T7 TE
     misc_feature    972..972
                     /label=stop
     polyA_site      966..979
                     /label=polya
     misc_feature    939..979
                     /label=BBa_B0012
     terminator      939..979
                     /label=BBa_B0012
     terminator      851..979
                     /label=BBa_B0015
     misc_feature    988..1007
                     /label=LuxR/HSL
     promoter        1007..1012
                     /label=-35
     promoter        1029..1034
                     /label=-10
     misc_feature    1040..1040
                     /label=start
     misc_feature    988..1042
                     /label=BBa_R0062
     promoter        988..1042
                     /label=BBa_R0062
     misc_feature    44..1042
                     /label=BBa_K317037
     misc_feature    1055..1058
                     /label=conserved
     RBS             1051..1062
                     /label=BBa_B0034
     CDS             1069..1713
                     /label=cheZ
     misc_feature    1069..1763
                     /label=BBa_K094100
     misc_feature    1764..1781
                     /label=6-His tag
     CDS             1069..1781
                     /label=BBa_K283008
     stem_loop       1801..1844
                     /label=stem_loop
     misc_feature    1790..1869
                     /label=BBa_B0010
     terminator      1790..1869
                     /label=BBa_B0010
     stem_loop       1885..1904
                     /label=T7 TE
     misc_feature    1911..1911
                     /label=stop
     polyA_site      1905..1918
                     /label=polya
     misc_feature    1878..1918
                     /label=BBa_B0012
     terminator      1878..1918
                     /label=BBa_B0012
     misc_feature    1051..1918
                     /label=BBa_K283030
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag
      121 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata
      181 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga
      241 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc
      301 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa
      361 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac
      421 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca
      481 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt
      541 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa
      601 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc
      661 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa
      721 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac
      781 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc
      841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa
     1021 tggtttgtta tagtcgaata aatactagag aaagaggaga aatactagat gatgcaacca
     1081 tcaatcaaac ctgctgacga gcattcagct ggcgatatca ttgcgcgcat cggcagcctg
     1141 acgcgtatgc tgcgcgacag tttgcgggaa ctggggctgg atcaggccat tgccgaagcg
     1201 gcggaagcca tccccgatgc gcgcgatcgt ttgtactatg ttgtgcagat gaccgcccag
     1261 gctgcggagc gggcgctgaa cagtgttgag gcgtcacaac cgcatcagga tcaaatggag
     1321 aaatcagcaa aagcgttaac ccaacgttgg gatgactggt ttgccgatcc gattgacctt
     1381 gccgacgccc gtgaactggt aacagataca cgacaatttc tggcagatgt acccgcgcat
     1441 accagcttta ctaacgcgca actgctggaa atcatgatgg cgcaggattt tcaggatctc
     1501 accgggcagg tcattaagcg gatgatggat gtcattcagg agatcgaacg ccagttgctg
     1561 atggtgctgt tggaaaacat cccggaacag gagtcgcgtc caaaacgtga aaaccagagt
     1621 ttgcttaatg gacctcaggt cgataccagc aaagccggtg tggtagccag tcaggatcag
     1681 gtggacgatt tgttggatag tcttggattt tgagctagca acgcaatgac gtcggaattg
     1741 ccagctgggg cgccctctgg taacatcatc accatcacca ctactagagc caggcatcaa
     1801 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1861 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//