##gff-version 3
##sequence-region BBa_K543010 1 1918
BBa_K543010	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_K543010	.	engineered_region	1051	1918	.	+	0	ID=BBa_K283030;Name=BBa_K283030
BBa_K543010	.	CDS	1069	1781	.	+	0	ID=BBa_K283008;Name=BBa_K283008;Parent=BBa_K283030
BBa_K543010	.	CDS	1069	1713	.	+	0	ID=annotation2035289;Name=cheZ;Parent=BBa_K283008
BBa_K543010	.	engineered_region	1069	1763	.	+	0	ID=annotation2035290;Name=BBa_K094100;Parent=BBa_K283008
BBa_K543010	.	tag	1764	1781	.	+	0	ID=annotation2035366;Name=6-His tag;Parent=BBa_K283008
BBa_K543010	.	terminator	1790	1869	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K283030
BBa_K543010	.	stem_loop	1801	1844	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K543010	.	engineered_region	1790	1869	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K543010	.	ribosome_entry_site	1051	1062	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K283030
BBa_K543010	.	conserved_region	1055	1058	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K543010	.	terminator	1878	1918	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K283030
BBa_K543010	.	polyA_site	1905	1918	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K543010	.	stem_loop	1885	1904	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K543010	.	engineered_region	1878	1918	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K543010	.	stop_codon	1911	1911	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K543010	.	engineered_region	44	1042	.	+	0	ID=BBa_K317037;Name=BBa_K317037
BBa_K543010	.	CDS	62	817	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K317037
BBa_K543010	.	engineered_region	62	817	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K543010	.	engineered_tag	818	842	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K543010	.	engineered_region	62	63	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K543010	.	point_mutation	553	553	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K543010	.	CDS	62	811	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K543010	.	point_mutation	235	235	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K543010	.	promoter	988	1042	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_K317037
BBa_K543010	.	promoter	1007	1012	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K543010	.	engineered_region	988	1042	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K543010	.	promoter	1029	1034	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K543010	.	start_codon	1040	1040	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K543010	.	non_covalent_binding_site	988	1007	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K543010	.	terminator	851	979	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K317037
BBa_K543010	.	terminator	939	979	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K543010	.	polyA_site	966	979	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K543010	.	stem_loop	946	965	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K543010	.	engineered_region	939	979	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K543010	.	stop_codon	972	972	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K543010	.	terminator	851	930	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K543010	.	stem_loop	862	905	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K543010	.	engineered_region	851	930	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K543010	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K317037
BBa_K543010	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K543010
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgaaaaacataaatgcc
gacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatga
ctaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcct
agataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattct
aactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaag
aagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttt
tgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttct
ctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtt
tagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttcca
tttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaatt
gattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttg
ttatagtcgaataaatactagagaaagaggagaaatactagatgatgcaaccatcaatcaaacctgctgacgagcattc
agctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcag
gccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctg
cggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaaccca
acgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctg
gcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctca
ccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacat
cccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggt
gtggtagccagtcaggatcaggtggacgatttgttggatagtcttggattttgagctagcaacgcaatgacgtcggaat
tgccagctggggcgccctctggtaacatcatcaccatcaccactactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttata