LOCUS       BBa_K543012             1926 bp    DNA     linear       25-Nov-2024
DEFINITION  plux-GFP (AHL inducible)
ACCESSION   BBa_K543012
VERSION     BBa_K543012.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..63
                     /label=prefix
     misc_feature    235..235
                     /label=T
     misc_feature    553..553
                     /label=A
     CDS             62..811
                     /label=luxR
     misc_feature    62..817
                     /label=BBa_C0062
     misc_feature    818..842
                     /label=Help:Barcodes
     CDS             62..817
                     /label=BBa_C0062
     stem_loop       862..905
                     /label=stem_loop
     misc_feature    851..930
                     /label=BBa_B0010
     terminator      851..930
                     /label=BBa_B0010
     stem_loop       946..965
                     /label=T7 TE
     misc_feature    972..972
                     /label=stop
     polyA_site      966..979
                     /label=polya
     misc_feature    939..979
                     /label=BBa_B0012
     terminator      939..979
                     /label=BBa_B0012
     terminator      851..979
                     /label=BBa_B0015
     misc_feature    988..1007
                     /label=LuxR/HSL
     promoter        1007..1012
                     /label=-35
     promoter        1029..1034
                     /label=-10
     misc_feature    1040..1040
                     /label=start
     misc_feature    988..1042
                     /label=BBa_R0062
     promoter        988..1042
                     /label=BBa_R0062
     misc_feature    44..1042
                     /label=BBa_K317037
     RBS             1051..1063
                     /label=RBS-3\Weak
     RBS             1057..1060
                     /label=RBS
     misc_feature    1051..1063
                     /label=BBa_B0032
     RBS             1051..1063
                     /label=BBa_B0032
     CDS             1070..1789
                     /label=GFP protein
     CDS             1070..1789
                     /label=BBa_E0040
     stem_loop       1809..1852
                     /label=stem_loop
     misc_feature    1798..1877
                     /label=BBa_B0010
     terminator      1798..1877
                     /label=BBa_B0010
     stem_loop       1893..1912
                     /label=T7 TE
     misc_feature    1919..1919
                     /label=stop
     polyA_site      1913..1926
                     /label=polya
     misc_feature    1886..1926
                     /label=BBa_B0012
     terminator      1886..1926
                     /label=BBa_B0012
     misc_feature    1051..1926
                     /label=BBa_E0240
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag
      121 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata
      181 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga
      241 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc
      301 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa
      361 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac
      421 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca
      481 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt
      541 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa
      601 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc
      661 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa
      721 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac
      781 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc
      841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa
     1021 tggtttgtta tagtcgaata aatactagag tcacacagga aagtactaga tgcgtaaagg
     1081 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
     1141 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
     1201 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
     1261 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt
     1321 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
     1381 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
     1441 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
     1501 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa
     1561 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca
     1621 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca
     1681 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt
     1741 aacagctgct gggattacac atggcatgga tgaactatac aaataataat actagagcca
     1801 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1861 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1921 tttata
//