LOCUS       BBa_K543013             1875 bp    DNA     linear       06-Dec-2025
DEFINITION  cheZ generator (AHL inducible) without promoter
ACCESSION   BBa_K543013
VERSION     BBa_K543013.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..20
                     /label=prefix
     misc_feature    192..192
                     /label=T
     misc_feature    510..510
                     /label=A
     CDS             19..768
                     /label=luxR
     misc_feature    19..774
                     /label=BBa_C0062
     misc_feature    775..799
                     /label=Help:Barcodes
     CDS             19..774
                     /label=BBa_C0062
     stem_loop       819..862
                     /label=stem_loop
     misc_feature    808..887
                     /label=BBa_B0010
     terminator      808..887
                     /label=BBa_B0010
     stem_loop       903..922
                     /label=T7 TE
     misc_feature    929..929
                     /label=stop
     polyA_site      923..936
                     /label=polya
     misc_feature    896..936
                     /label=BBa_B0012
     terminator      896..936
                     /label=BBa_B0012
     terminator      808..936
                     /label=BBa_B0015
     misc_feature    945..964
                     /label=LuxR/HSL
     promoter        964..969
                     /label=-35
     promoter        986..991
                     /label=-10
     misc_feature    997..997
                     /label=start
     misc_feature    945..999
                     /label=BBa_R0062
     promoter        945..999
                     /label=BBa_R0062
     misc_feature    1..999
                     /label=BBa_K317037
     misc_feature    1012..1015
                     /label=conserved
     RBS             1008..1019
                     /label=BBa_B0034
     CDS             1026..1670
                     /label=cheZ
     misc_feature    1026..1720
                     /label=BBa_K094100
     misc_feature    1721..1738
                     /label=6-His tag
     CDS             1026..1738
                     /label=BBa_K283008
     stem_loop       1758..1801
                     /label=stem_loop
     misc_feature    1747..1826
                     /label=BBa_B0010
     terminator      1747..1826
                     /label=BBa_B0010
     stem_loop       1842..1861
                     /label=T7 TE
     misc_feature    1868..1868
                     /label=stop
     polyA_site      1862..1875
                     /label=polya
     misc_feature    1835..1875
                     /label=BBa_B0012
     terminator      1835..1875
                     /label=BBa_B0012
     misc_feature    1008..1875
                     /label=BBa_K283030
ORIGIN
        1 aaagaggaga aatactagat gaaaaacata aatgccgacg acacatacag aataattaat
       61 aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa
      121 atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct
      181 gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat
      241 ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc aattaattgg
      301 aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa
      361 acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga
      421 atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg
      481 tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa aataaatata
      541 gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca
      601 tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc
      661 actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt
      721 tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta ataacactga
      781 tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga
      841 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      901 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagacctgt aggatcgtac
      961 aggtttacgc aagaaaatgg tttgttatag tcgaataaat actagagaaa gaggagaaat
     1021 actagatgat gcaaccatca atcaaacctg ctgacgagca ttcagctggc gatatcattg
     1081 cgcgcatcgg cagcctgacg cgtatgctgc gcgacagttt gcgggaactg gggctggatc
     1141 aggccattgc cgaagcggcg gaagccatcc ccgatgcgcg cgatcgtttg tactatgttg
     1201 tgcagatgac cgcccaggct gcggagcggg cgctgaacag tgttgaggcg tcacaaccgc
     1261 atcaggatca aatggagaaa tcagcaaaag cgttaaccca acgttgggat gactggtttg
     1321 ccgatccgat tgaccttgcc gacgcccgtg aactggtaac agatacacga caatttctgg
     1381 cagatgtacc cgcgcatacc agctttacta acgcgcaact gctggaaatc atgatggcgc
     1441 aggattttca ggatctcacc gggcaggtca ttaagcggat gatggatgtc attcaggaga
     1501 tcgaacgcca gttgctgatg gtgctgttgg aaaacatccc ggaacaggag tcgcgtccaa
     1561 aacgtgaaaa ccagagtttg cttaatggac ctcaggtcga taccagcaaa gccggtgtgg
     1621 tagccagtca ggatcaggtg gacgatttgt tggatagtct tggattttga gctagcaacg
     1681 caatgacgtc ggaattgcca gctggggcgc cctctggtaa catcatcacc atcaccacta
     1741 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1801 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1861 ctttctgcgt ttata
//