##gff-version 3
##sequence-region BBa_K543013 1 1875
BBa_K543013	.	engineered_region	1	999	.	+	0	ID=BBa_K317037;Name=BBa_K317037
BBa_K543013	.	CDS	19	774	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K317037
BBa_K543013	.	engineered_region	19	774	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K543013	.	engineered_tag	775	799	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K543013	.	engineered_region	19	20	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K543013	.	point_mutation	510	510	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K543013	.	CDS	19	768	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K543013	.	point_mutation	192	192	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K543013	.	promoter	945	999	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_K317037
BBa_K543013	.	promoter	964	969	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K543013	.	engineered_region	945	999	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K543013	.	promoter	986	991	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K543013	.	start_codon	997	997	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K543013	.	non_covalent_binding_site	945	964	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K543013	.	terminator	808	936	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K317037
BBa_K543013	.	terminator	896	936	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K543013	.	polyA_site	923	936	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K543013	.	stem_loop	903	922	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K543013	.	engineered_region	896	936	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K543013	.	stop_codon	929	929	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K543013	.	terminator	808	887	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K543013	.	stem_loop	819	862	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K543013	.	engineered_region	808	887	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K543013	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K317037
BBa_K543013	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K543013	.	engineered_region	1008	1875	.	+	0	ID=BBa_K283030;Name=BBa_K283030
BBa_K543013	.	CDS	1026	1738	.	+	0	ID=BBa_K283008;Name=BBa_K283008;Parent=BBa_K283030
BBa_K543013	.	CDS	1026	1670	.	+	0	ID=annotation2035289;Name=cheZ;Parent=BBa_K283008
BBa_K543013	.	engineered_region	1026	1720	.	+	0	ID=annotation2035290;Name=BBa_K094100;Parent=BBa_K283008
BBa_K543013	.	tag	1721	1738	.	+	0	ID=annotation2035366;Name=6-His tag;Parent=BBa_K283008
BBa_K543013	.	terminator	1747	1826	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K283030
BBa_K543013	.	stem_loop	1758	1801	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K543013	.	engineered_region	1747	1826	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K543013	.	ribosome_entry_site	1008	1019	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K283030
BBa_K543013	.	conserved_region	1012	1015	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K543013	.	terminator	1835	1875	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K283030
BBa_K543013	.	polyA_site	1862	1875	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K543013	.	stem_loop	1842	1861	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K543013	.	engineered_region	1835	1875	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K543013	.	stop_codon	1868	1868	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_K543013
aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaa
gcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattta
tcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgct
aatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaaca
atgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctat
tcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttta
catgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaat
caaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaa
aatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgc
caaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagt
gtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacct
gtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagat
gatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatg
ctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatc
gtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatca
ggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgac
gcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgc
tggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagat
cgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttg
cttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagtc
ttggattttgagctagcaacgcaatgacgtcggaattgccagctggggcgccctctggtaacatcatcaccatcaccac
tactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtg
aacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata