LOCUS       BBa_K543015              885 bp    DNA     linear       25-Nov-2024
DEFINITION  TetR generator (J23100-B0031-R0040-B0015)
ACCESSION   BBa_K543015
VERSION     BBa_K543015.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    50..53
                     /label=conserved
     RBS             44..57
                     /label=BBa_B0031
     CDS             67..683
                     /label=tetR
     misc_feature    684..717
                     /label=SsrA
     misc_feature    724..748
                     /label=Help:Barcodes
     CDS             64..723
                     /label=BBa_C0040
     stem_loop       768..811
                     /label=stem_loop
     misc_feature    757..836
                     /label=BBa_B0010
     terminator      757..836
                     /label=BBa_B0010
     stem_loop       852..871
                     /label=T7 TE
     misc_feature    878..878
                     /label=stop
     polyA_site      872..885
                     /label=polya
     misc_feature    845..885
                     /label=BBa_B0012
     terminator      845..885
                     /label=BBa_B0012
     misc_feature    44..885
                     /label=BBa_P0140
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagtcacaca ggaaacctac
       61 tagatgtcca gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag
      121 gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct
      181 acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg
      241 ttagataggc accatactca cttttgccct ttagaagggg aaagctggca agatttttta
      301 cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta
      361 catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt
      421 ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat
      481 tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa
      541 acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac
      601 caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa
      661 caacttaaat gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt agtagcttaa
      721 taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg
      781 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      841 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//