LOCUS       BBa_K543016             1838 bp    DNA     linear       25-Nov-2024
DEFINITION  Bacteriophage Lysis Cassette S105, R, and Rz under Tet promoter  
            (conjugative)
ACCESSION   BBa_K543016
VERSION     BBa_K543016.1  
FEATURES             Location/Qualifiers
     oriT            1..355
                     /label=BBa_I714030
     misc_feature    364..382
                     /label=TetR 1
     misc_feature    364..417
                     /label=BBa_R0040
     promoter        383..388
                     /label=-35
     misc_feature    389..407
                     /label=TetR 2
     promoter        406..411
                     /label=-10
     promoter        364..417
                     /label=BBa_R0040
     RBS             426..438
                     /label=RBS-3\Weak
     RBS             432..435
                     /label=RBS
     misc_feature    426..438
                     /label=BBa_B0032
     RBS             426..438
                     /label=BBa_B0032
     CDS             445..1701
                     /label=BBa_K124017
     stem_loop       1721..1764
                     /label=stem_loop
     misc_feature    1710..1789
                     /label=BBa_B0010
     terminator      1710..1789
                     /label=BBa_B0010
     stem_loop       1805..1824
                     /label=T7 TE
     misc_feature    1831..1831
                     /label=stop
     polyA_site      1825..1838
                     /label=polya
     misc_feature    1798..1838
                     /label=BBa_B0012
     terminator      1798..1838
                     /label=BBa_B0012
     terminator      1710..1838
                     /label=BBa_B0015
ORIGIN
        1 cctctggtga ctttatccgt aaataattta acccactcca caaaaaggct caacaggttg
       61 gtggttctca ccaccaaaag caccacaccc cacgcaaaaa caagtttttg ctgatttttc
      121 tttataaata gagtgttatg aaaaattagt ttctcttact ctctttatga tatttaaaaa
      181 agcggtgtcg gcgcggctac aacaacgcgc cgacaccgtt ttgtaggggt ggtactgact
      241 atttttataa aaaacattat tttatattag gggtgctgct agcggcgcgg tgtgtttttt
      301 tataggatac cgctaggggc gctgctagcg gtgcgtccct gtttgcatta tgaattacta
      361 gagtccctat cagtgataga gattgacatc cctatcagtg atagagatac tgagcactac
      421 tagagtcaca caggaaagta ctagatgcca gaaaaacatg acctgttggc cgccattctc
      481 gcggcaaagg aacaaggcat cggggcaatc cttgcgtttg caatggcgta ccttcgcggc
      541 agatataatg gcggtgcgtt tacaaaaaca gtaatcgacg caacgatgtg cgccattatc
      601 gcctggttca ttcgtgacct tctcgacttc gccggactaa gtagcaatct cgcttatata
      661 acgagcgtgt ttatcggcta catcggtact gactcgattg gttcgcttat caaacgcttc
      721 gctgctaaaa aagccggagt agaagatggt agaaatcaat aaatggtaga aatcaataat
      781 caacgtaagg cgttcctcga tatgctggcg tggtcggagg gaactgataa cggacgtcag
      841 aaaaccagaa atcatggtta tgacgtcatt gtaggcggag agctatttac tgattactcc
      901 gatcaccctc gcaaacttgt cacgctaaac ccaaaactca aatcaacagg cgccggacgc
      961 taccagcttc tttcccgttg gtgggatgcc taccgcaagc agcttggcct gaaagacttc
     1021 tctccgaaaa gtcaggacgc tgtggcattg cagcagatta aggagcgtgg cgctttacct
     1081 atgattgatc gtggtgatat ccgtcaggca atcgaccgtt gcagcaatat ctgggcttca
     1141 ctgccgggcg ctggttatgg tcagttcgag cataaggctg acagcctgat tgcaaaattc
     1201 aaagaagcgg gcggaacggt cagagagatt gatgtatgaa tgagcagagt caccgcgatt
     1261 atctccgctc tggttatctg catcatcgtc tgcctgtcat gggctgttaa tcattaccgt
     1321 gataacgcca ttacctacaa agcccagcgc gacaaaaatg ccagagaact gaagctggcg
     1381 aacgcggcaa ttactgacat gcagatgcgt cagcgtgatg ttgctgcgct cgatgcaaaa
     1441 tacacgaagg agttagctga tgctaaagct gaaaatgatg ctctgcgtga tgatgttgcc
     1501 gctggtcgtc gtcggttgca catcaaagca gtctgtcagt cagtgcgtga agccaccacc
     1561 gcctccggcg tggataatgc agcctccccc cgactggcag acaccgctga acgggattat
     1621 ttcaccctca gagagaggct gatcactatg caaaaacaac tggaaggaac ccagaagtat
     1681 attaatgagc agtgcagata gtactagagc caggcatcaa ataaaacgaa aggctcagtc
     1741 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1801 cactggctca ccttcgggtg ggcctttctg cgtttata
//