##gff-version 3
##sequence-region BBa_K543016 1 1838
BBa_K543016	.	promoter	364	417	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K543016	.	engineered_region	364	417	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K543016	.	promoter	383	388	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K543016	.	promoter	406	411	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K543016	.	non_covalent_binding_site	364	382	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K543016	.	non_covalent_binding_site	389	407	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K543016	.	CDS	445	1701	.	+	0	ID=BBa_K124017;Name=BBa_K124017
BBa_K543016	.	terminator	1710	1838	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K543016	.	terminator	1798	1838	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K543016	.	polyA_site	1825	1838	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K543016	.	engineered_region	1798	1838	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K543016	.	stem_loop	1805	1824	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K543016	.	stop_codon	1831	1831	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K543016	.	terminator	1710	1789	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K543016	.	engineered_region	1710	1789	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K543016	.	stem_loop	1721	1764	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K543016	.	oriT	1	355	.	+	0	ID=BBa_I714030;Name=BBa_I714030
BBa_K543016	.	ribosome_entry_site	426	438	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K543016	.	ribosome_entry_site	426	438	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K543016	.	ribosome_entry_site	432	435	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K543016	.	engineered_region	426	438	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
>BBa_K543016
cctctggtgactttatccgtaaataatttaacccactccacaaaaaggctcaacaggttggtggttctcaccaccaaaa
gcaccacaccccacgcaaaaacaagtttttgctgatttttctttataaatagagtgttatgaaaaattagtttctctta
ctctctttatgatatttaaaaaagcggtgtcggcgcggctacaacaacgcgccgacaccgttttgtaggggtggtactg
actatttttataaaaaacattattttatattaggggtgctgctagcggcgcggtgtgtttttttataggataccgctag
gggcgctgctagcggtgcgtccctgtttgcattatgaattactagagtccctatcagtgatagagattgacatccctat
cagtgatagagatactgagcactactagagtcacacaggaaagtactagatgccagaaaaacatgacctgttggccgcc
attctcgcggcaaaggaacaaggcatcggggcaatccttgcgtttgcaatggcgtaccttcgcggcagatataatggcg
gtgcgtttacaaaaacagtaatcgacgcaacgatgtgcgccattatcgcctggttcattcgtgaccttctcgacttcgc
cggactaagtagcaatctcgcttatataacgagcgtgtttatcggctacatcggtactgactcgattggttcgcttatc
aaacgcttcgctgctaaaaaagccggagtagaagatggtagaaatcaataaatggtagaaatcaataatcaacgtaagg
cgttcctcgatatgctggcgtggtcggagggaactgataacggacgtcagaaaaccagaaatcatggttatgacgtcat
tgtaggcggagagctatttactgattactccgatcaccctcgcaaacttgtcacgctaaacccaaaactcaaatcaaca
ggcgccggacgctaccagcttctttcccgttggtgggatgcctaccgcaagcagcttggcctgaaagacttctctccga
aaagtcaggacgctgtggcattgcagcagattaaggagcgtggcgctttacctatgattgatcgtggtgatatccgtca
ggcaatcgaccgttgcagcaatatctgggcttcactgccgggcgctggttatggtcagttcgagcataaggctgacagc
ctgattgcaaaattcaaagaagcgggcggaacggtcagagagattgatgtatgaatgagcagagtcaccgcgattatct
ccgctctggttatctgcatcatcgtctgcctgtcatgggctgttaatcattaccgtgataacgccattacctacaaagc
ccagcgcgacaaaaatgccagagaactgaagctggcgaacgcggcaattactgacatgcagatgcgtcagcgtgatgtt
gctgcgctcgatgcaaaatacacgaaggagttagctgatgctaaagctgaaaatgatgctctgcgtgatgatgttgccg
ctggtcgtcgtcggttgcacatcaaagcagtctgtcagtcagtgcgtgaagccaccaccgcctccggcgtggataatgc
agcctccccccgactggcagacaccgctgaacgggattatttcaccctcagagagaggctgatcactatgcaaaaacaa
ctggaaggaacccagaagtatattaatgagcagtgcagatagtactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttata