LOCUS       BBa_K543020             1254 bp    DNA     linear       07-May-2024
DEFINITION  OriTr-GFP
ACCESSION   BBa_K543020
VERSION     BBa_K543020.1  
FEATURES             Location/Qualifiers
     oriT            1..371
                     /label=BBa_J01003
     misc_feature    384..387
                     /label=conserved
     RBS             380..391
                     /label=BBa_B0034
     CDS             398..1117
                     /label=GFP protein
     CDS             398..1117
                     /label=BBa_E0040
     stem_loop       1137..1180
                     /label=stem_loop
     misc_feature    1126..1205
                     /label=BBa_B0010
     terminator      1126..1205
                     /label=BBa_B0010
     stem_loop       1221..1240
                     /label=T7 TE
     misc_feature    1247..1247
                     /label=stop
     polyA_site      1241..1254
                     /label=polya
     misc_feature    1214..1254
                     /label=BBa_B0012
     terminator      1214..1254
                     /label=BBa_B0012
     misc_feature    380..1254
                     /label=BBa_I13504
ORIGIN
        1 gccgcctttt cctcaatcgc tcttcgttcg tctggaaggc agtacacctt gataggtggg
       61 ctgcccttcc tggttggctt ggtttcatca gccatccgct tgccctcatc tgttacgccg
      121 gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa
      181 gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc
      241 tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat
      301 cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat
      361 gcagcggaaa atactagaga aagaggagaa atactagatg cgtaaaggag aagaactttt
      421 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
      481 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg
      541 cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt
      601 tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat
      661 gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac
      721 acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat
      781 tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca
      841 caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag
      901 acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat
      961 tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc
     1021 gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg
     1081 gattacacat ggcatggatg aactatacaa ataataatac tagagccagg catcaaataa
     1141 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1201 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//